| Project/Area Number |
23370094
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Developmental biology
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| Research Institution | Okazaki Research Facilities, National Institutes of Natural Sciences |
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Principal Investigator |
TAKADA Shinji 大学共同利用機関法人自然科学研究機構(岡崎共通研究施設), 岡崎統合バイオサイエンスセンター, 教授 (60206753)
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| Co-Investigator(Renkei-kenkyūsha) |
YABE Taijiro 基礎生物学研究所, 分子発生学研究部門, 助教 (30470074)
TAKADA Ritsuko 大学共同利用機関法人自然科学研究機構(岡崎共通研究施設), 岡崎統合バイオサイエンスセンター, 特別協力研究員 (40450720)
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥20,020,000 (Direct Cost: ¥15,400,000、Indirect Cost: ¥4,620,000)
Fiscal Year 2013: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2012: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000)
Fiscal Year 2011: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
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| Keywords | シグナル伝達 / 発生・分化 / 遺伝子 / Wnt / 形態形成 / 細胞・組織 |
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| Research Abstract |
Secreted signaling proteins play various roles in many biological phenomena. However, important questions, such as how these proteins are distributed and how their distribution is regulated, still remain to be elucidated probably because of limitation of sensitivity of experimental analyses. As one of the several approaches to answer these questions, we carefully examined spatial distribution of Wnt-3a proteins in developing mouse spinal cord by modified immunohistochemistry, identified a molecule that regulates spatial distribution of Wnt proteins using Xenopus embryo as an assay system, and examined its function in embryos. Furthermore, we also revealed that Wnt proteins are secreted in different forms according to cell types and cell polarity. These results reveal heterogeneity of Wnt proteins in spatial distribution and secreted forms.
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