Analysis of the proteins possessed by the last common ancestor and the organisms present before it
Project/Area Number |
23370097
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Evolutionary biology
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Research Institution | Tokyo University of Pharmacy and Life Science |
Principal Investigator |
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Co-Investigator(Kenkyū-buntansha) |
YOKOBORI Shin-ichi 東京薬科大学, 生命科学部, 講師 (40291702)
AKANUMA Satoshi 東京薬科大学, 生命科学部, 助教 (10321720)
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Project Period (FY) |
2011-11-18 – 2014-03-31
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Project Status |
Completed (Fiscal Year 2013)
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Budget Amount *help |
¥10,140,000 (Direct Cost: ¥7,800,000、Indirect Cost: ¥2,340,000)
Fiscal Year 2013: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2012: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
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Keywords | 生命初期進化 / 共通祖先 / コモノート / NDK / 分子系統樹 / タンパク質耐熱性 / 酵素活性 / アミノ酸単純化 / 古細菌 / 真正細菌 / アミノ酸少数化 |
Research Abstract |
We have resurrected the gene of the nucleotidediphosphate kinase (NDK) possessed by the common ancestor of Bacteria and Archaea, and analyzed the thermal stability of the protein produced from the resurrected genes. The ancestral NDKs showed stability close to 110 degree C, suggesting the common ancestors of Bacteria and Archaea were thriving at the temperature between 80 and 100 degree C. The NDK of the last common ancestor, commonote, was also resurrected and reproduced. The ancestral NDK showed the high stability, suggesting that the commonote were living at 75 degree C or higher temperature. The ancestral NDKs from which one of the 20 amino acid species were removed, were produced and whose stability and activity were analyzed. The results showed the amino acid species that is not absolutely required for the stability and activity.
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Report
(4 results)
Research Products
(76 results)
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[Journal Article] Evaluation of the protein interfaces that form an intermolecular four-helix bundle as studied by computer simulation2014
Author(s)
Fukuda, M., Komatsu, Y., Yamada, H., Morikawa, R., Miyakawa, T., Takasu, M., Akanuma, S., Yamagishi, A
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Journal Title
Molecular Simulation
Volume: 40
Issue: 6
Pages: 498-503
DOI
Related Report
Peer Reviewed
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[Journal Article] Characterization of the low-temperature activity of Sulfolobus tokodaii glucose-1-dehydrogenase mutants2014
Author(s)
Sugii, T., Akanuma, S., Yagi, S., Yagyu, K., Shimoda, Y., Yamagishi, A.
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Journal Title
J. Biosci. Bioeng
Volume: ***
Issue: 4
Pages: 16010-16010
DOI
NAID
Related Report
Peer Reviewed
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[Journal Article] Experimental evidence for the thermophilicity of ancestral life2013
Author(s)
Akanuma, S., Nakajima, Y., Yokobori, S., Kimura, M., Nemoto, N., Mase, T., Miyazono, K., Tanokura, M., Yamagishi A
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Journal Title
Proc. Natl. Acad. Sci. USA
Volume: 110(27)
Pages: 11067-11072
Related Report
Peer Reviewed
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[Journal Article] Phylogeny-based design of a B-subunit of DNA gyrase and its ATPase domain using a small set of homologous amino acid sequences2011
Author(s)
Akanuma, S., Iwami, S., Yokoi, T., Nakamura, N., Watanabe, H., Yokobori, S. and Yamagishi, A
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Journal Title
J. Mol. Biol
Volume: 412,(2)
Pages: 212-225
Related Report
Peer Reviewed
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[Book] 化学同人2013
Author(s)
山岸明彦 編著
Total Pages
332
Publisher
アストロバイオロジー 宇宙に生命の起源をもとめて
Related Report
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