Analysis of transporters and transcription factors controlling suberization in Casparian strip in rice

• Project/Area Number: 23380004
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Breeding science
• Research Institution: Nagoya University
• Principal Investigator: NAKAZONO Mikio 名古屋大学, 生命農学研究科, 教授 (70282697)
• Project Period (FY): 2011-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥19,370,000 (Direct Cost: ¥14,900,000、Indirect Cost: ¥4,470,000); Fiscal Year 2013: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2012: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000); Fiscal Year 2011: ¥9,880,000 (Direct Cost: ¥7,600,000、Indirect Cost: ¥2,280,000)
• Keywords: イネ / カスパリー線 / スベリン / ABCトランスポーター

Research Abstract

In rice, the hypodermis in the outer part of roots forms a suberized cell wall (Casparian strip and/or suberin lamellae), which inhibits the flow of water and ions. We examined if an ABC transporter RCN1 is involved in the Casparian strip and suberin lamellae formations and tried to understand the mechanism of suberization in Casparian strip and suberin lamellae by identifying transcription factors, which control expressions of suberin biosynthetic genes and suberin transporter genes. Under stagnant deoxygenated conditions, well-suberized hypodermis developed in the wild type but not in the rcn1 mutant. An apoplastic tracer, periodic acid, was blocked at the hypodermis in the wild type but not in rcn1, indicating that the apoplastic barrier in the mutant was impaired. Moreover, we identified some transcription factors, which might control the formations of Casparian strip and suberin lamellae.

Research Products

• [Journal Article] Rice alcohol dehydrogenase 1 promotes survival and has a major impact on carbohydrate metabolism in the embryo and endosperm when seeds are germinated in partially oxygenated water (2014)
  • Author(s): Takahashi, H., Greenway, H., Matsumura, H., T sutsumi N. and Nakazono, M
  • Journal Title: Annals of Botany Volume: 113 Pages: 851-859
  • Peer Reviewed
• [Journal Article] Strigolactone and cytokinin act antagonistically in regulating rice mesocotyl elongation in darkness (2014)
  • Author(s): Hu, Z., Y amauchi, T ., Y ang, J., Jikumaru, Y ., T suchida-Mayama, T ., Ichikawa, H., T akamure, I., Nagamura, Y ., T sutsumi, N., Y amaguchi, S., Kyozuka J. and Nakazono, M.
  • Journal Title: Plant and Cell Physiology Volume: 55 Pages: 30-41
  • Peer Reviewed
• [Journal Article] Ethylene and reactive oxygen species are involved in root aerenchyma formation and adaptation of wheat seedlings to oxygen-deficient conditions (2014)
  • Author(s): Yamauchi, T ., Watanabe, K., Fukazawa, A., Mori, H., Abe, F ., Kawaguchi, K., Oyanagi, A. and Nakazono, M
  • Journal Title: Journal of Experimental Botany Volume: 65 Pages: 261-273
  • Peer Reviewed
• [Journal Article] Effects of organic acids on formation of the barrier to radial oxygen loss in roots of Hordeum marinum (2014)
  • Author(s): Kotula, L., Colmer, T .D. and Nakazono, M
  • Journal Title: Functional Plant Biology Volume: 41 Pages: 187-202
  • Peer Reviewed
• [Journal Article] Does suberin accumulation in plant roots contribute to waterlogging tolerance? (2013)
  • Author(s): Watanabe, K., Nishiuchi, S., Kulichikhin, K. and Nakazono, M
  • Journal Title: Frontiers in Plant Science Volume: 4 Pages: 178-178
  • Peer Reviewed
• [Journal Article] Aerenchyma formation in crop species : a review (2013)
  • Author(s): Yamauchi, T ., Shimamura, S., Nakazono, M. and Mochizuki, T
  • Journal Title: Field Crops Research Volume: 152 Pages: 8-16
  • Peer Reviewed
• [Journal Article] Screening of candidate genes associated with constitutive aerenchyma formation in adventitious roots of the teosinte Zea nicaraguensis (2012)
  • Author(s): Abiko, T ., Obara, M., Abe, F ., Kawaguchi, K., Oyanagi, A., Y amauchi, T . and Nakazono, M
  • Journal Title: Plant Root Volume: 6 Pages: 19-27
  • NAID: 130002077479
  • Peer Reviewed
• [Journal Article] Enhanced formation of aerenchyma and induction of a barrier to radial oxygen loss in adventitious roots of Zea nica r a guensis contribute to its waterlogging tolerance as compared with maize (Zea ma y s ssp. ma y s). Plant (2012)
  • Author(s): Abiko, T ., Kotula, L., Shiono, K., Malik, A.I., Colmer, T .D. and Nakazono, M
  • Journal Title: Cell & Environment Volume: 35 Pages: 1618-1630
  • Peer Reviewed
• [Journal Article] Mechanisms for coping with submergence and waterlogging in rice (2012)
  • Author(s): Nishiuchi, S., Y amauchi, T ., T akahashi, H., Kotula, L. and Nakazono, M
  • Journal Title: Rice Volume: 5 Pages: 2-2
  • Peer Reviewed
• [Journal Article] Enhanced formation of aerenchyma and induction of a barrier to radial oxygen loss in adventitious roots of Zea nicaraguensis contribute to its waterlogging tolerance as compared with maize (Zea mays ssp.mays.) (2012)
  • Author(s): Abiko, T., Kotula, L., Shiono, K., Malik, A.I., Colmer, T.D., Nakazono, M.
  • Journal Title: Plant Cell Environ Volume: (in press) Issue: 9 Pages: 1618-1630
  • DOI: 10.1111/j.1365-3040.2012.02513.x
  • Peer Reviewed
• [Journal Article] Mechanisms for coping with submergence and waterlogging in rice (2012)
  • Author(s): Nishiuchi, S., Yamauchi, T., Takahashi, H., Kotula, L., Nakazono, M.
  • Journal Title: Rice Volume: 5 Issue: 1 Pages: 2-2
  • DOI: 10.1186/1939-8433-5-2
  • Peer Reviewed
• [Journal Article] Cell division and cell elongation in the coleoptile of rice alcohol dehydrogenase 1-deficient mutant are reduced under complete submergence (2011)
  • Author(s): Takahashi, H., Saika, H., Matsumura, H., Nagamura, Y ., T sutsumi, N., Nishizawa, N.K. and Nakazono, M
  • Journal Title: Annals of Botany Volume: 108 Pages: 253-261
  • Peer Reviewed
• [Journal Article] Identification of genes expressed in maize root cortical cells during lysigenous aerenchyma formation using laser microdissection and microarray analyses (2011)
  • Author(s): Rajhi, I., Y amauchi, T ., T akahashi, H., Nishiuchi, S., Shiono, K., Watanabe, R., Mliki, A., Nagamura, Y ., T sutsumi, N., Nishizawa, N.K. and Nakazono, M
  • Journal Title: New Phytologist Volume: 190 Pages: 351-368
  • Peer Reviewed
• [Journal Article] Contrasting dynamics of radial O2-loss barrier induction and aerenchyma formation in rice roots of two lengths (2011)
  • Author(s): Shiono, K., Ogawa, S., Y amazaki, S., Isoda, H., Fujimura, T ., Nakazono, M. and Colmer, T .D
  • Journal Title: Annals of Botany Volume: 107 Pages: 89-99
  • Peer Reviewed
• [Journal Article] Microarray analysis of laser-microdissected tissues indicates the biosynthesis of suberin in the outer part of roots during formation of a barrier to radial oxygen loss in rice (Oryzasativa)
  • Author(s): Shiono, K., Y amauchi, T, Y amazaki, S., Mohanty, B., Malik, A.I., Nagamura, Y ., Nishizawa, N.K., T sutsumi, N., Colmer, T .D. and Nakazono, M
  • Journal Title: Journal of Experimental Botany Volume: (印刷中)
  • Peer Reviewed
• [Journal Article] Adventitious roots of wheat seedlings that emerge in oxygen-deficient conditions have increased root diameters with highly developed lysigenous aerenchyma
  • Author(s): Yamauchi, T ., Abe, F ., Kawaguchi, K., Oyanagi, A. and Nakazono, M.
  • Journal Title: Plant Signaling & Behavior Volume: (印刷中)
  • Peer Reviewed
• [Journal Article] Biochemical and molecular characterization of rice (OryzasativaL.) roots forming a barrier to radial oxygen loss
  • Author(s): Kulichikhin, K., Y amauchi, T ., Watanabe, K. and Nakazono, M
  • Journal Title: Plant, Cell & Environment Volume: (印刷中)
  • Peer Reviewed
• [Presentation] Role of the NAC transcriptional factor OsNAC34 in formation of a barrier to radial oxygen loss in rice (2013)
  • Author(s): Shunsaku Nishiuchi, Kohtaro Watanabe, Katsuhiro Shiono, Tomoko T suchida-Mayama, Nobutaka Mitsuda, Masaru Ohme-Takagi, Hiroaki Ichikawa, Mikio Nakazono
  • Organizer: 11th conference of the International Society for Plant Anaerobiosis
  • Place of Presentation: Los Banos, Philippines
• [Presentation] イネの根のスベリンラメラ形成過程に発現するCYP86 遺伝子およびGPAT 遺伝子の探索 (2013)
  • Author(s): 渡邊宏太郎
  • Organizer: 第123回 日本育種学会講演会
  • Place of Presentation: 東京農業大学
• [Presentation] 嫌気条件下のイネの根におけるスベリン生合成関連遺伝子群の発現変動 (2012)
  • Author(s): 渡邊 宏太郎, 西内 俊策, 中園 幹生
  • Organizer: 第20回育種学会中部地区談話会
  • Place of Presentation: 名古屋
  • Year and Date: 2012-12-08
• [Presentation] NAM型転写因子OsNAC34のイネのスベリン生合成への関与 (2012)
  • Author(s): 西内 俊策, 渡邊 宏太郎, 塩野 克宏, 槌田(間山) 智子, 光田 展隆, 高木 優, 市川 裕章, 中園 幹生
  • Organizer: 第122回日本育種学会講演会
  • Place of Presentation: 京都
• [Presentation] 嫌気条件下のイネの根におけるスベリン生合成関連遺伝子群の発現解析 (2012)
  • Author(s): 渡邊 宏太郎, 西内 俊策, 中園 幹生
  • Organizer: 第121回日本育種学会講演会
  • Place of Presentation: 宇都宮
• [Presentation] NAM型転写因子OsNAC34のイネのスベリン生合成への関与 (2012)
  • Author(s): 西内俊策
  • Organizer: 第122回 日本育種学会講演会
  • Place of Presentation: 京都産業大学（京都）
• [Presentation] イネの根におけるスベリン生合成関連遺伝子群の詳細な解析 (2012)
  • Author(s): 渡邊宏太郎
  • Organizer: 第122回 日本育種学会講演会
  • Place of Presentation: 京都産業大学（京都）
• [Presentation] Identification of an A TP-binding cassette (ABC) transporter that is required for formation of suberin lamellae and the apoplastic barrier at the hypodermis in rice (Oryzasativa) (2011)
  • Author(s): Katsuhiro Shiono, Miho Ando, Motoaki Nakamura, Y uichi Matsuo, Naoko Y asuno, Hirokazu T akahashi, Shunsaku Nishiuchi, Masaru Fujimoto, Hideki T akanashi, Kosala Ranathunge, Rochus Franke, Nobukazu Shitan, Naoko K Nishizawa, Itsuro T akamure, Nobuhiro T sutsumi, Lukas Schreiber , Kazufumi Y azaki, Mikio Nakazono, Kiyoaki Kato
  • Organizer: The JSRR's 20th Anniversary Symposium-The Latest Frontiers of Root Research in Asia
  • Place of Presentation: 東京
• [Presentation] イネのスベリン生合成経路に関与するNAM型転写因子の機能解析 (2011)
  • Author(s): 西内 俊策, 渡邊 宏太郎, 山内 卓樹, 高橋 宏和, 塩野 克宏, 槌田(間山) 智子, 光田 展隆, 高木 優, 市川 裕章, 中園 幹生
  • Organizer: 第120回日本育種学会講演会
  • Place of Presentation: 福井