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Analysis of transporters and transcription factors controlling suberization in Casparian strip in rice

Research Project

Project/Area Number 23380004
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Breeding science
Research InstitutionNagoya University

Principal Investigator

NAKAZONO Mikio  名古屋大学, 生命農学研究科, 教授 (70282697)

Project Period (FY) 2011-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥19,370,000 (Direct Cost: ¥14,900,000、Indirect Cost: ¥4,470,000)
Fiscal Year 2013: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2012: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2011: ¥9,880,000 (Direct Cost: ¥7,600,000、Indirect Cost: ¥2,280,000)
Keywordsイネ / カスパリー線 / スベリン / ABCトランスポーター
Research Abstract

In rice, the hypodermis in the outer part of roots forms a suberized cell wall (Casparian strip and/or suberin lamellae), which inhibits the flow of water and ions. We examined if an ABC transporter RCN1 is involved in the Casparian strip and suberin lamellae formations and tried to understand the mechanism of suberization in Casparian strip and suberin lamellae by identifying transcription factors, which control expressions of suberin biosynthetic genes and suberin transporter genes. Under stagnant deoxygenated conditions, well-suberized hypodermis developed in the wild type but not in the rcn1 mutant. An apoplastic tracer, periodic acid, was blocked at the hypodermis in the wild type but not in rcn1, indicating that the apoplastic barrier in the mutant was impaired. Moreover, we identified some transcription factors, which might control the formations of Casparian strip and suberin lamellae.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Annual Research Report
  • 2011 Annual Research Report
  • Research Products

    (26 results)

All 2014 2013 2012 2011 Other

All Journal Article (17 results) (of which Peer Reviewed: 17 results) Presentation (9 results)

  • [Journal Article] Rice alcohol dehydrogenase 1 promotes survival and has a major impact on carbohydrate metabolism in the embryo and endosperm when seeds are germinated in partially oxygenated water2014

    • Author(s)
      Takahashi, H., Greenway, H., Matsumura, H., T sutsumi N. and Nakazono, M
    • Journal Title

      Annals of Botany

      Volume: 113 Pages: 851-859

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Strigolactone and cytokinin act antagonistically in regulating rice mesocotyl elongation in darkness2014

    • Author(s)
      Hu, Z., Y amauchi, T ., Y ang, J., Jikumaru, Y ., T suchida-Mayama, T ., Ichikawa, H., T akamure, I., Nagamura, Y ., T sutsumi, N., Y amaguchi, S., Kyozuka J. and Nakazono, M.
    • Journal Title

      Plant and Cell Physiology

      Volume: 55 Pages: 30-41

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Ethylene and reactive oxygen species are involved in root aerenchyma formation and adaptation of wheat seedlings to oxygen-deficient conditions2014

    • Author(s)
      Yamauchi, T ., Watanabe, K., Fukazawa, A., Mori, H., Abe, F ., Kawaguchi, K., Oyanagi, A. and Nakazono, M
    • Journal Title

      Journal of Experimental Botany

      Volume: 65 Pages: 261-273

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Effects of organic acids on formation of the barrier to radial oxygen loss in roots of Hordeum marinum2014

    • Author(s)
      Kotula, L., Colmer, T .D. and Nakazono, M
    • Journal Title

      Functional Plant Biology

      Volume: 41 Pages: 187-202

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Does suberin accumulation in plant roots contribute to waterlogging tolerance?2013

    • Author(s)
      Watanabe, K., Nishiuchi, S., Kulichikhin, K. and Nakazono, M
    • Journal Title

      Frontiers in Plant Science

      Volume: 4 Pages: 178-178

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Aerenchyma formation in crop species : a review2013

    • Author(s)
      Yamauchi, T ., Shimamura, S., Nakazono, M. and Mochizuki, T
    • Journal Title

      Field Crops Research

      Volume: 152 Pages: 8-16

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Screening of candidate genes associated with constitutive aerenchyma formation in adventitious roots of the teosinte Zea nicaraguensis2012

    • Author(s)
      Abiko, T ., Obara, M., Abe, F ., Kawaguchi, K., Oyanagi, A., Y amauchi, T . and Nakazono, M
    • Journal Title

      Plant Root

      Volume: 6 Pages: 19-27

    • NAID

      130002077479

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Enhanced formation of aerenchyma and induction of a barrier to radial oxygen loss in adventitious roots of Zea nica r a guensis contribute to its waterlogging tolerance as compared with maize (Zea ma y s ssp. ma y s). Plant2012

    • Author(s)
      Abiko, T ., Kotula, L., Shiono, K., Malik, A.I., Colmer, T .D. and Nakazono, M
    • Journal Title

      Cell & Environment

      Volume: 35 Pages: 1618-1630

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Mechanisms for coping with submergence and waterlogging in rice2012

    • Author(s)
      Nishiuchi, S., Y amauchi, T ., T akahashi, H., Kotula, L. and Nakazono, M
    • Journal Title

      Rice

      Volume: 5 Pages: 2-2

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Enhanced formation of aerenchyma and induction of a barrier to radial oxygen loss in adventitious roots of Zea nicaraguensis contribute to its waterlogging tolerance as compared with maize (Zea mays ssp.mays.)2012

    • Author(s)
      Abiko, T., Kotula, L., Shiono, K., Malik, A.I., Colmer, T.D., Nakazono, M.
    • Journal Title

      Plant Cell Environ

      Volume: (in press) Issue: 9 Pages: 1618-1630

    • DOI

      10.1111/j.1365-3040.2012.02513.x

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Mechanisms for coping with submergence and waterlogging in rice2012

    • Author(s)
      Nishiuchi, S., Yamauchi, T., Takahashi, H., Kotula, L., Nakazono, M.
    • Journal Title

      Rice

      Volume: 5 Issue: 1 Pages: 2-2

    • DOI

      10.1186/1939-8433-5-2

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Cell division and cell elongation in the coleoptile of rice alcohol dehydrogenase 1-deficient mutant are reduced under complete submergence2011

    • Author(s)
      Takahashi, H., Saika, H., Matsumura, H., Nagamura, Y ., T sutsumi, N., Nishizawa, N.K. and Nakazono, M
    • Journal Title

      Annals of Botany

      Volume: 108 Pages: 253-261

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Identification of genes expressed in maize root cortical cells during lysigenous aerenchyma formation using laser microdissection and microarray analyses2011

    • Author(s)
      Rajhi, I., Y amauchi, T ., T akahashi, H., Nishiuchi, S., Shiono, K., Watanabe, R., Mliki, A., Nagamura, Y ., T sutsumi, N., Nishizawa, N.K. and Nakazono, M
    • Journal Title

      New Phytologist

      Volume: 190 Pages: 351-368

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Contrasting dynamics of radial O2-loss barrier induction and aerenchyma formation in rice roots of two lengths2011

    • Author(s)
      Shiono, K., Ogawa, S., Y amazaki, S., Isoda, H., Fujimura, T ., Nakazono, M. and Colmer, T .D
    • Journal Title

      Annals of Botany

      Volume: 107 Pages: 89-99

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Microarray analysis of laser-microdissected tissues indicates the biosynthesis of suberin in the outer part of roots during formation of a barrier to radial oxygen loss in rice (Oryzasativa)

    • Author(s)
      Shiono, K., Y amauchi, T, Y amazaki, S., Mohanty, B., Malik, A.I., Nagamura, Y ., Nishizawa, N.K., T sutsumi, N., Colmer, T .D. and Nakazono, M
    • Journal Title

      Journal of Experimental Botany

      Volume: (印刷中)

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Adventitious roots of wheat seedlings that emerge in oxygen-deficient conditions have increased root diameters with highly developed lysigenous aerenchyma

    • Author(s)
      Yamauchi, T ., Abe, F ., Kawaguchi, K., Oyanagi, A. and Nakazono, M.
    • Journal Title

      Plant Signaling & Behavior

      Volume: (印刷中)

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Biochemical and molecular characterization of rice (OryzasativaL.) roots forming a barrier to radial oxygen loss

    • Author(s)
      Kulichikhin, K., Y amauchi, T ., Watanabe, K. and Nakazono, M
    • Journal Title

      Plant, Cell & Environment

      Volume: (印刷中)

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Presentation] Role of the NAC transcriptional factor OsNAC34 in formation of a barrier to radial oxygen loss in rice2013

    • Author(s)
      Shunsaku Nishiuchi, Kohtaro Watanabe, Katsuhiro Shiono, Tomoko T suchida-Mayama, Nobutaka Mitsuda, Masaru Ohme-Takagi, Hiroaki Ichikawa, Mikio Nakazono
    • Organizer
      11th conference of the International Society for Plant Anaerobiosis
    • Place of Presentation
      Los Banos, Philippines
    • Related Report
      2013 Final Research Report
  • [Presentation] イネの根のスベリンラメラ形成過程に発現するCYP86 遺伝子およびGPAT 遺伝子の探索2013

    • Author(s)
      渡邊宏太郎
    • Organizer
      第123回 日本育種学会講演会
    • Place of Presentation
      東京農業大学
    • Related Report
      2012 Annual Research Report
  • [Presentation] 嫌気条件下のイネの根におけるスベリン生合成関連遺伝子群の発現変動2012

    • Author(s)
      渡邊 宏太郎, 西内 俊策, 中園 幹生
    • Organizer
      第20回育種学会中部地区談話会
    • Place of Presentation
      名古屋
    • Year and Date
      2012-12-08
    • Related Report
      2013 Final Research Report
  • [Presentation] NAM型転写因子OsNAC34のイネのスベリン生合成への関与2012

    • Author(s)
      西内 俊策, 渡邊 宏太郎, 塩野 克宏, 槌田(間山) 智子, 光田 展隆, 高木 優, 市川 裕章, 中園 幹生
    • Organizer
      第122回日本育種学会講演会
    • Place of Presentation
      京都
    • Related Report
      2013 Final Research Report
  • [Presentation] 嫌気条件下のイネの根におけるスベリン生合成関連遺伝子群の発現解析2012

    • Author(s)
      渡邊 宏太郎, 西内 俊策, 中園 幹生
    • Organizer
      第121回日本育種学会講演会
    • Place of Presentation
      宇都宮
    • Related Report
      2013 Final Research Report
  • [Presentation] NAM型転写因子OsNAC34のイネのスベリン生合成への関与2012

    • Author(s)
      西内俊策
    • Organizer
      第122回 日本育種学会講演会
    • Place of Presentation
      京都産業大学(京都)
    • Related Report
      2012 Annual Research Report
  • [Presentation] イネの根におけるスベリン生合成関連遺伝子群の詳細な解析2012

    • Author(s)
      渡邊宏太郎
    • Organizer
      第122回 日本育種学会講演会
    • Place of Presentation
      京都産業大学(京都)
    • Related Report
      2012 Annual Research Report
  • [Presentation] Identification of an A TP-binding cassette (ABC) transporter that is required for formation of suberin lamellae and the apoplastic barrier at the hypodermis in rice (Oryzasativa)2011

    • Author(s)
      Katsuhiro Shiono, Miho Ando, Motoaki Nakamura, Y uichi Matsuo, Naoko Y asuno, Hirokazu T akahashi, Shunsaku Nishiuchi, Masaru Fujimoto, Hideki T akanashi, Kosala Ranathunge, Rochus Franke, Nobukazu Shitan, Naoko K Nishizawa, Itsuro T akamure, Nobuhiro T sutsumi, Lukas Schreiber , Kazufumi Y azaki, Mikio Nakazono, Kiyoaki Kato
    • Organizer
      The JSRR's 20th Anniversary Symposium-The Latest Frontiers of Root Research in Asia
    • Place of Presentation
      東京
    • Related Report
      2013 Final Research Report
  • [Presentation] イネのスベリン生合成経路に関与するNAM型転写因子の機能解析2011

    • Author(s)
      西内 俊策, 渡邊 宏太郎, 山内 卓樹, 高橋 宏和, 塩野 克宏, 槌田(間山) 智子, 光田 展隆, 高木 優, 市川 裕章, 中園 幹生
    • Organizer
      第120回日本育種学会講演会
    • Place of Presentation
      福井
    • Related Report
      2013 Final Research Report

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Published: 2011-04-06   Modified: 2019-07-29  

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