Development of genomics assisted breeding system of salt tolerant barley

• Project/Area Number: 23380007
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Breeding science
• Research Institution: Okayama University
• Principal Investigator: SATO Kazuhiro 岡山大学, その他部局等, 教授 (60215770)
• Co-Investigator(Kenkyū-buntansha): HISANO Hiroshi 岡山大学, 資源生物科学研究所, 助教 (70415454)
• Project Period (FY): 2011-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥19,240,000 (Direct Cost: ¥14,800,000、Indirect Cost: ¥4,440,000); Fiscal Year 2013: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2012: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000); Fiscal Year 2011: ¥8,060,000 (Direct Cost: ¥6,200,000、Indirect Cost: ¥1,860,000)
• Keywords: 耐塩性 / 不良環境耐性 / オオムギ / ＤＮＡマーカー / 遺伝子単離 / 形質転換 / cDNA / DNAマーカー / 育種学 / ゲノム

Research Abstract

cDNA sequences are generated by an efficient analysis system and aligned with full length cDNA sequences of Haruna Nijo to develop genome wide SNP detection system. The array system is used to genotype recombinant inbred lines and recombinant chromosome substitution lines for genetic map development and estimation of substitution segments. Salt tolerance of these lines are evaluated to map tolerance QTLs and their responsible substitution segments. On the other hand, a previously isolated tolerance gene by subtraction method is used to make transgenic plant to evaluate its salt tolerance. Markers are also developed to narrow down tolerance genes. The recombinant chromosome substitution liens carrying tolerance gene segments are developed to be used as isogenic lines for salt tolerance.

Research Products

• [Journal Article] Mapping non-recombining regions in barley using multicolor FISH (2013)
  • Author(s): Karafiá tová M., Bartoš J., Kopecký D., Ma L., Sato K., Houben A., Stein N. and Dolež el J.
  • Journal Title: Chromosome Res Volume: 21 Issue: 8 Pages: 739-751
  • DOI: 10.1007/s10577-013-9380-x
  • Peer Reviewed
• [Journal Article] オオムギゲノム多様性の解析と育種への応用 (2013)
  • Author(s): 佐藤和広
  • Journal Title: 育種学研究 Volume: 15 Pages: 167-172
  • NAID: 130004850620
• [Journal Article] Discovery of high-confidence single nucleotide polymorphisms from large-scale de novo analysis of leaf transcripts of Aegilops tauschii, a wild wheat progenitor (2012)
  • Author(s): Iehisa JCM, Shimizu A, Sato K, Nasuda S, Takumi S
  • Journal Title: DNA Research Volume: 19 Issue: 6 Pages: 487-497
  • DOI: 10.1093/dnares/dss028
  • NAID: 120005398237
  • Peer Reviewed
• [Journal Article] A physical, genetic and functional sequence assembly of the barley genome (2012)
  • Author(s): The International Barley Genome Sequencing Consortium (IBSC)
  • Journal Title: Nature Volume: 491 Issue: 7426 Pages: 711-716
  • DOI: 10.1038/nature11543
  • Peer Reviewed
• [Journal Article] Development of genetic map and alignment of recombinant chromosome substitution lines from a cross of EST donors by high accuracy SNP typing in barley (2011)
  • Author(s): Sato, K., Close, T., Bhat, P., Muñ oz-Amatriaí n, M., and Muehlbauer, G
  • Journal Title: Plant Cell Physiol Volume: 52 Pages: 728-737
  • Peer Reviewed
• [Journal Article] Construction and sequence analysis of barley 24, 783 full-length cDNAs (2011)
  • Author(s): Matsumoto, T, Tanaka, T., Sakai, H., Amano, N., Kanamori, H., Kurita, K., Kikuta, A., Kamiya, K., Yamamoto, M., Ikawa, H., Fujii, N., Hori, K., Itoh, T., Takeda, K., and Sato, K
  • Journal Title: Plant Physiol Volume: 156 Issue: 1 Pages: 20-28
  • DOI: 10.1104/pp.110.171579
  • Peer Reviewed
• [Journal Article] Unlocking an archetypal 5.1 Gb Triticeae genome by barley chromosomal genomics (2011)
  • Author(s): Mayer, K., et al.
  • Journal Title: Plant Cell Volume: 23 Issue: 4 Pages: 1249-1263
  • DOI: 10.1105/tpc.110.082537
  • Peer Reviewed
• [Journal Article] An improved consensus linkage map of barley based on flow-sorted chromosomes and SNP markers (2011)
  • Author(s): Muñ oz-Amatriaín, M., et al.
  • Journal Title: The Plant Genome Volume: Vol.4No.3 Issue: 3 Pages: 238-249
  • DOI: 10.3835/plantgenome2011.08.0023
  • Peer Reviewed
• [Presentation] Evaluation and use of stress tolerance in barley genetic resources (2014)
  • Author(s): Kazuhiro Sato
  • Organizer: Visions for Nordic pre-breeding collaboration, PPP Seminar & Partner meeting
  • Place of Presentation: Radisson SAS, Reykjavik
  • Invited
• [Presentation] オオムギゲノム多様性の解析と育種への応用 (平成24年度日本育種学会賞受賞講演) (2013)
  • Author(s): 佐藤和広
  • Organizer: 日本育種学会講演会
  • Place of Presentation: 東京
• [Presentation] Barley natural variation and adaptation to global environments (2013)
  • Author(s): Kazuhiro Sato
  • Organizer: 16th Australian Barley Technical Symposium, Sofitel Melbourne on Collins
  • Place of Presentation: Melbourne, Australia
• [Presentation] オオムギ遺伝資源コレクションの全ゲノム SNP フィンガープリンティング (2013)
  • Author(s): 佐藤和広・元井由加
  • Organizer: 日本育種学会講演会
  • Place of Presentation: 鹿児島
• [Presentation] Barley natural variation and adaptation to global environments (2013)
  • Author(s): Kazuhiro Sato
  • Organizer: 16th Australian Barley Technical Symposium
  • Place of Presentation: Sofitel Melbourne on Collins
  • Invited
• [Presentation] オオムギゲノム多様性の解析と育種への応用 (2013)
  • Author(s): 佐藤和広
  • Organizer: 日本育種学会
  • Place of Presentation: 東京農業大学
  • Invited
• [Presentation] cDNA 解析によるオオムギゲノム育種用 DNA マーカーの開発 (2012)
  • Author(s): 佐藤和広・元井由加
  • Organizer: 日本育種学会講演会
  • Place of Presentation: 京都
• [Presentation] オオムギゲノム配列の概要 (2012)
  • Author(s): 佐藤和広
  • Organizer: ムギ類研究会
  • Place of Presentation: つくば
• [Book] 植物の遺伝と育種第2版 (2013)
  • Author(s): 福井希一・向井康比古・佐藤和広
  • Total Pages: 238
  • Publisher: 朝倉書店
• [Book] Genetic diversity and germplasm management in "Biotechnological Approaches to Barley Improvement" (Kumlehn and Stein eds.) (2013)
  • Author(s): KazuhiroSato et al.
  • Publisher: Springer
• [Book] Genetic diversity and germplasm management -wild barley, landraces, breeding materials. Kumlehn, J., and Stein, N., (eds.) Biotechnological Approaches to Barley Improvement. Biotechnology in Agriculture and Forestry
  • Author(s): Sato, K., Flavell, A., Russell, J., Bö rner, A., and Valkoun, J
  • Publisher: Springer(印刷中)