Development of genomics assisted breeding system of salt tolerant barley
| Project/Area Number |
23380007
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Breeding science
|
|---|
| Research Institution | Okayama University |
|---|
Principal Investigator |
SATO Kazuhiro 岡山大学, その他部局等, 教授 (60215770)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
HISANO Hiroshi 岡山大学, 資源生物科学研究所, 助教 (70415454)
|
|---|
| Project Period (FY) |
2011-04-01 – 2014-03-31
|
| Project Status |
Completed (Fiscal Year 2013)
|
| Budget Amount *help |
¥19,240,000 (Direct Cost: ¥14,800,000、Indirect Cost: ¥4,440,000)
Fiscal Year 2013: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2012: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Fiscal Year 2011: ¥8,060,000 (Direct Cost: ¥6,200,000、Indirect Cost: ¥1,860,000)
|
|---|
| Keywords | 耐塩性 / 不良環境耐性 / オオムギ / ＤＮＡマーカー / 遺伝子単離 / 形質転換 / cDNA / DNAマーカー / 育種学 / ゲノム |
|---|
| Research Abstract |
cDNA sequences are generated by an efficient analysis system and aligned with full length cDNA sequences of Haruna Nijo to develop genome wide SNP detection system. The array system is used to genotype recombinant inbred lines and recombinant chromosome substitution lines for genetic map development and estimation of substitution segments. Salt tolerance of these lines are evaluated to map tolerance QTLs and their responsible substitution segments. On the other hand, a previously isolated tolerance gene by subtraction method is used to make transgenic plant to evaluate its salt tolerance. Markers are also developed to narrow down tolerance genes. The recombinant chromosome substitution liens carrying tolerance gene segments are developed to be used as isogenic lines for salt tolerance.
|
Report
(4 results)
Research Products
(19 results)
-
-
-
-
-
-
[Journal Article] Construction and sequence analysis of barley 24, 783 full-length cDNAs2011
Author(s)
Matsumoto, T, Tanaka, T., Sakai, H., Amano, N., Kanamori, H., Kurita, K., Kikuta, A., Kamiya, K., Yamamoto, M., Ikawa, H., Fujii, N., Hori, K., Itoh, T., Takeda, K., and Sato, K
-
Journal Title
Plant Physiol
Volume: 156
Issue: 1
Pages: 20-28
DOI
Related Report
Peer Reviewed
-
-
-
-
-
-
-
-
-
-
-
-
-
[Book] Genetic diversity and germplasm management -wild barley, landraces, breeding materials. Kumlehn, J., and Stein, N., (eds.) Biotechnological Approaches to Barley Improvement. Biotechnology in Agriculture and Forestry
Author(s)
Sato, K., Flavell, A., Russell, J., Bö rner, A., and Valkoun, J
Publisher
Springer(印刷中)
Related Report
