Establishment of a new breeding procedure based on rice gene targeting using information of molecular genetics and attempt to generate a new variety.
| Project/Area Number |
23380008
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Breeding science
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| Research Institution | Meijo University |
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Principal Investigator |
TERADA Rie 名城大学, 農学部, 教授 (30137799)
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥18,980,000 (Direct Cost: ¥14,600,000、Indirect Cost: ¥4,380,000)
Fiscal Year 2013: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2012: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2011: ¥10,400,000 (Direct Cost: ¥8,000,000、Indirect Cost: ¥2,400,000)
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| Keywords | 植物分子育種学 / 遺伝子ターゲティング / イネ / ポジティブ・ネガティブ選抜 / マーカー削除 / Cre/loxP / 部位特異的組換え / プロモーター / マーカーフリー / ヒ素耐性 / 耐病性 / Cre-loxP |
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| Research Abstract |
For generation of a rice new variety by gene targeting (GT) procedure, the OsRac1 was modified by GT based on positive-negative selection to generate blast fungus resistance rice. Since the G19V substitution in OsRac1 had shown the effective immunity in random transgenic rice, the same modification at the original OsRac1 locus was examined. Following to GT for G19V substitution positive-marker remained in the OsRac1 locus was removed by Cre-loxP mediated marker free system. The OsRac1 activation was detected in the gene-modified rice plants of segregated homozygous lines for the G to T alternation in the exon 1, however, unexpected growth inhibition in the plants was also associated. For generation of arsenic resistant rice, the 16 of rice plant lines having PvACR were obtained.
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Report
(4 results)
Research Products
(14 results)
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[Journal Article] Targeted disruption of an orthologue of DOMAINS REARRANGED METHYLASE2, OsDRM2, impairs the growth of rice plants by abnormal DNA methylation2012
Author(s)
Moritoh, S., Eun, C-H, Ono, A., Asao, H., Okano, Y., Yamaguchi, K., Shimatani, Z., Koizumi, A. and Terada R
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Journal Title
The Plant Journal
Volume: 71
Issue: 1
Pages: 85-98
DOI
Related Report
Peer Reviewed
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