| Budget Amount *help |
¥18,850,000 (Direct Cost: ¥14,500,000、Indirect Cost: ¥4,350,000)
Fiscal Year 2014: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2013: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2012: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Fiscal Year 2011: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
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| Outline of Final Research Achievements |
For the molecular analysis of appearing mechanism of aposporous embryo sac initial cell (AIC) in apomictic guinea grass in this study, 1) the AIC was isolated after ovaries treated with enzyme solution and by this its existence was proofed; 2) the apomixis specific gene (ASG-1) isolated from apomict of guinea grass (showing multiple embryo sac formation (MES)) was used for plasmid construction of hsp::ASG-1::GFP to produce transformants of Arabidopsis. In T4∼T5 generations,1) GFP expression was observed in each organism; 2) in experiments of either rapid or long time drought treatment, the plants showed drought resistance comparing to the non-transformants; 3) the following phenomena in the same ovule were observed in part of the plants (20%): a) MES formation; b) multiple embryogenesis (ME) (one embryo and the embryoid initiated from micropylar end); c) ME (one embryo from micropylar end, and the embryoid from the chalazal end).
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