| Project/Area Number |
23380017
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Horticulture/Landscape architecture
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| Research Institution | Kyoto University |
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Principal Investigator |
YAMANE Hisayo 京都大学, (連合)農学研究科(研究院), 講師 (80335306)
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| Co-Investigator(Kenkyū-buntansha) |
TAO Ryutaro 京都大学, 大学院農学研究科, 准教授 (10211997)
HABU Tsuyoshi 愛媛大学, 農学部, 准教授 (60335304)
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| Research Collaborator |
AKAGI Takashi
NAGAYAMA Erika
HOSAKA Yukari
SASAKI Ryuta
TAKEUCHI Nozomi
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| Project Period (FY) |
2011-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥16,510,000 (Direct Cost: ¥12,700,000、Indirect Cost: ¥3,810,000)
Fiscal Year 2013: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2012: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2011: ¥9,620,000 (Direct Cost: ¥7,400,000、Indirect Cost: ¥2,220,000)
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| Keywords | 果樹 / 休眠 / 転写因子 / 組換え遺伝子 / 遺伝子解析技術 / バイオインフォマティクス / 環境応答 / 低温要求性 / MADS-box / 温度 |
|---|
| Outline of Final Research Achievements |
Bud dormancy of temperate fruit trees is one of the important agronomic traits that affects next season’s annual growth. In this study, we used 454-pyrosequencing technique to gather sequence information of dormant bud ESTs in Prunus mume and to search for the candidate genes that are involved in endodormancy regulation. As a result, PmDAM4 and PmDAM5 were found to be up-regulated in endodormant buds as is the case with PmDAM6 which has already been identified as endodormancy-related gene in our previous studies. All six PmDAM genes were up-regulated when chilling exposure was avoided and down-regulated during cold treatment. Among them, PmDAM4, PmDAM5, and PmDAM6 were down-regulated by cold treatment in chilling requirement-dependent manner. These results suggested that PmDAMs play roles in genetic control of chilling requirement of Prunus mume.
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