Studies on MADS-box transcription factors associated with bud dormancy regulation in the fruit tree species of Prunus
| Project/Area Number |
23380017
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Horticulture/Landscape architecture
|
|---|
| Research Institution | Kyoto University |
|---|
Principal Investigator |
YAMANE Hisayo 京都大学, (連合)農学研究科(研究院), 講師 (80335306)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
TAO Ryutaro 京都大学, 大学院農学研究科, 准教授 (10211997)
HABU Tsuyoshi 愛媛大学, 農学部, 准教授 (60335304)
|
|---|
| Research Collaborator |
AKAGI Takashi
NAGAYAMA Erika
HOSAKA Yukari
SASAKI Ryuta
TAKEUCHI Nozomi
|
|---|
| Project Period (FY) |
2011-04-01 – 2015-03-31
|
| Project Status |
Completed (Fiscal Year 2014)
|
| Budget Amount *help |
¥16,510,000 (Direct Cost: ¥12,700,000、Indirect Cost: ¥3,810,000)
Fiscal Year 2013: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2012: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2011: ¥9,620,000 (Direct Cost: ¥7,400,000、Indirect Cost: ¥2,220,000)
|
|---|
| Keywords | 果樹 / 休眠 / 転写因子 / 組換え遺伝子 / 遺伝子解析技術 / バイオインフォマティクス / 環境応答 / 低温要求性 / MADS-box / 温度 |
|---|
| Outline of Final Research Achievements |
Bud dormancy of temperate fruit trees is one of the important agronomic traits that affects next season’s annual growth. In this study, we used 454-pyrosequencing technique to gather sequence information of dormant bud ESTs in Prunus mume and to search for the candidate genes that are involved in endodormancy regulation. As a result, PmDAM4 and PmDAM5 were found to be up-regulated in endodormant buds as is the case with PmDAM6 which has already been identified as endodormancy-related gene in our previous studies. All six PmDAM genes were up-regulated when chilling exposure was avoided and down-regulated during cold treatment. Among them, PmDAM4, PmDAM5, and PmDAM6 were down-regulated by cold treatment in chilling requirement-dependent manner. These results suggested that PmDAMs play roles in genetic control of chilling requirement of Prunus mume.
|
Report
(4 results)
Research Products
(22 results)
