| Budget Amount *help |
¥18,980,000 (Direct Cost: ¥14,600,000、Indirect Cost: ¥4,380,000)
Fiscal Year 2014: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2013: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2012: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2011: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
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| Outline of Final Research Achievements |
Self-incompatibility in Japanese pear is controlled by S-haplotype at the S-locus, which code a pistil S determinant, S-RNase, and pollen S determinants, S-locus F-box brothers (PpSFBBs). In this study, we expanded BAC contigs around S-RNase to find at least 17 PpSFBB2s coded by S2-haplotype. PpSFBB2 homologs cloned from pollen of S homozygous lines had high identity between haplotypes, which predicts that the collaborative non-self S-RNase recognition model in Solanaceae species fits the S-RNase-based SI systems in the Japanese pear. Using the immune microscopy, we confirmed endocytic uptake of S-RNase into pollen tube, and subsequent trafficking via endosomes.
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