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Development of pollen S-specificity in self-incompatibility in Japanese pear

Research Project

Project/Area Number 23380018
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Horticultural science
Research InstitutionKobe University

Principal Investigator

TAKASAKI-YASUDA TAKESHI  神戸大学, (連合)農学研究科(研究院), 教授 (30314511)

Co-Investigator(Kenkyū-buntansha) ISHIMIZU Takeshi  立命館大学, 生命科学部, 准教授 (30314355)
Co-Investigator(Renkei-kenkyūsha) ISHIMIZU Takeshi  立命館大学, 生命科学部, 准教授 (30314355)
Project Period (FY) 2011-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥18,980,000 (Direct Cost: ¥14,600,000、Indirect Cost: ¥4,380,000)
Fiscal Year 2014: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2013: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2012: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2011: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Keywords自家不和合性 / ナシ / 果樹園芸学 / 分子遺伝学 / 細胞組織学 / 植物 / 自他認識 / 分子生物学 / 園芸学
Outline of Final Research Achievements

Self-incompatibility in Japanese pear is controlled by S-haplotype at the S-locus, which code a pistil S determinant, S-RNase, and pollen S determinants, S-locus F-box brothers (PpSFBBs). In this study, we expanded BAC contigs around S-RNase to find at least 17 PpSFBB2s coded by S2-haplotype. PpSFBB2 homologs cloned from pollen of S homozygous lines had high identity between haplotypes, which predicts that the collaborative non-self S-RNase recognition model in Solanaceae species fits the S-RNase-based SI systems in the Japanese pear. Using the immune microscopy, we confirmed endocytic uptake of S-RNase into pollen tube, and subsequent trafficking via endosomes.

Report

(5 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Annual Research Report
  • 2012 Annual Research Report
  • 2011 Annual Research Report
  • Research Products

    (19 results)

All 2015 2014 2013 2012 2011

All Journal Article (5 results) (of which Peer Reviewed: 5 results) Presentation (13 results) Book (1 results)

  • [Journal Article] Cloning an S-RNase allele, including the longest intron, from cultivars of European pear (Pyrus communis L.)2013

    • Author(s)
      Takasaki-Yasuda, T., Nomura, N., Moriya, Y., Okada, K., Iwanami, H. and Bessho, H.
    • Journal Title

      J. Hortic. Sci. Biotech.

      Volume: 88 Pages: 427-432

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] S-RNase-based genotyping of triploid cultivars of European pear (Pyrus communis L.)2013

    • Author(s)
      Takasaki-Yasuda, T., Nomura, N., Moriya, Y., Okada, K., Iwanami, H. and Bessho, H.
    • Journal Title

      J. Hortic. Sci. Biotech.

      Volume: 88 Pages: 751-755

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Dual recognition of S1 and S4 pistils by S4sm pollen in self-incompatibility of Japanese pears (Pyrus pyrifolia Nakai)2012

    • Author(s)
      Saito, T.
    • Journal Title

      Tree Genetics & Genomes

      Volume: 8 Issue: 4 Pages: 689-694

    • DOI

      10.1007/s11295-011-0456-5

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Related polymorphic F-box protein genes between haplotypes clustering in the BAC contig sequences around S-RNase of Japanese pear2011

    • Author(s)
      Okada, K
    • Journal Title

      Journal of Experimental Botany

      Volume: 62 Issue: 6 Pages: 1887-1902

    • DOI

      10.1093/jxb/erq381

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effects of L-arginine on solubilization and purification of plant membrane proteins2011

    • Author(s)
      Arakawa, J.
    • Journal Title

      Protein Expression and Purification

      Volume: 80 Issue: 1 Pages: 91-962

    • DOI

      10.1016/j.pep.2011.05.014

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Presentation] ニホンナシ和合・不和合花粉管へのS-RNaseの取り込み2014

    • Author(s)
      李麗怡・時安美奈・藤本龍・安田(高崎)剛志
    • Organizer
      園芸学会平成26年度秋季大会
    • Place of Presentation
      佐賀大学
    • Year and Date
      2014-09-27 – 2014-09-28
    • Related Report
      2014 Annual Research Report
  • [Presentation] ニホンナシnon-S-RNaseの花柱組織における蓄積と花粉管への取り込み2014

    • Author(s)
      時安美奈・李麗怡・岡村彩葉・藤本龍・安田(高崎)剛志
    • Organizer
      園芸学会平成26年度秋季大会
    • Place of Presentation
      佐賀大学
    • Year and Date
      2014-09-27 – 2014-09-28
    • Related Report
      2014 Annual Research Report
  • [Presentation] ニホンナシS4-RNase周辺BACコンティグの拡張によるPpSFBB遺伝子群の探索2014

    • Author(s)
      安藤肇・山下翔人・藤本龍・安田(高崎)剛志
    • Organizer
      園芸学会平成26年度秋季大会
    • Place of Presentation
      佐賀大学
    • Year and Date
      2014-09-27 – 2014-09-28
    • Related Report
      2014 Annual Research Report
  • [Presentation] ニホンナシSホモ系統の花粉cDNAからのPpSFBB2ホモログのクローニング2014

    • Author(s)
      西村遼太郎・今村剛士・加藤大貴・藤本龍・安田(高崎)剛志
    • Organizer
      園芸学会平成26年度秋季大会
    • Place of Presentation
      佐賀大学
    • Year and Date
      2014-09-27 – 2014-09-28
    • Related Report
      2014 Annual Research Report
  • [Presentation] ニホンナシにおける自家・他家受粉後の花粉管微細構造の変化2013

    • Author(s)
      李麗怡・時安美奈・朴杓允・安田(高崎)剛志
    • Organizer
      園芸学会平成25年度秋季大会
    • Place of Presentation
      岩手大学
    • Related Report
      2013 Annual Research Report
  • [Presentation] S2-RNase周辺BACコンティグの拡張によるPpSFBB遺伝子群の探索II2013

    • Author(s)
      藤田裕樹・ズムラット クルバン・齋藤寿広・安田(高崎)剛志
    • Organizer
      園芸学会平成25年度秋季大会
    • Place of Presentation
      岩手大学
    • Related Report
      2013 Annual Research Report
  • [Presentation] ニホンナシS4-RNase 周辺BAC コンティグの拡張による新規PpSFBB4 遺伝子の探索2013

    • Author(s)
      岡田和馬
    • Organizer
      園芸学会平成25年度春季大会
    • Place of Presentation
      東京農工大学
    • Related Report
      2012 Annual Research Report
  • [Presentation] ニホンナシ花柱組織の発育に伴うS-RNaseの蓄積2012

    • Author(s)
      岡村彩葉
    • Organizer
      園芸学会平成24年度秋季大会
    • Place of Presentation
      福井県立大学
    • Related Report
      2012 Annual Research Report
  • [Presentation] S2-RNase周辺BACコンティグの拡張によるPpSFBB遺伝子群の探索2012

    • Author(s)
      藤田裕樹
    • Organizer
      園芸学会平成24年度秋季大会
    • Place of Presentation
      福井県立大学
    • Related Report
      2012 Annual Research Report
  • [Presentation] ニホンナシ花柱組織の発育に伴う微細構造の変化2011

    • Author(s)
      岡村彩葉
    • Organizer
      園芸学会
    • Place of Presentation
      岡山大学
    • Year and Date
      2011-09-24
    • Related Report
      2011 Annual Research Report
  • [Presentation] ニホンナシの柱頭浸出液中にもS-RNaseが含まれている2011

    • Author(s)
      木村穣
    • Organizer
      園芸学会
    • Place of Presentation
      岡山大学
    • Year and Date
      2011-09-24
    • Related Report
      2011 Annual Research Report
  • [Presentation] ニホンナシにおける不和合花粉の発芽伸長は和合花粉に比べ遅延する2011

    • Author(s)
      山下翔人
    • Organizer
      園芸学会
    • Place of Presentation
      岡山大学
    • Year and Date
      2011-09-24
    • Related Report
      2011 Annual Research Report
  • [Presentation] セイヨウナシ数品種からの新規Sx-RNaseのクローニング2011

    • Author(s)
      野村直希
    • Organizer
      園芸学会
    • Place of Presentation
      岡山大学
    • Year and Date
      2011-09-24
    • Related Report
      2011 Annual Research Report
  • [Book] 果樹園芸学2015

    • Author(s)
      金浜耕基 編  分担
    • Total Pages
      314
    • Publisher
      文永堂
    • Related Report
      2014 Annual Research Report

URL: 

Published: 2011-04-06   Modified: 2019-07-29  

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