Development of pollen S-specificity in self-incompatibility in Japanese pear
| Project/Area Number |
23380018
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Horticultural science
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| Research Institution | Kobe University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
ISHIMIZU Takeshi 立命館大学, 生命科学部, 准教授 (30314355)
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| Co-Investigator(Renkei-kenkyūsha) |
ISHIMIZU Takeshi 立命館大学, 生命科学部, 准教授 (30314355)
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| Project Period (FY) |
2011-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥18,980,000 (Direct Cost: ¥14,600,000、Indirect Cost: ¥4,380,000)
Fiscal Year 2014: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2013: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2012: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2011: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
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| Keywords | 自家不和合性 / ナシ / 果樹園芸学 / 分子遺伝学 / 細胞組織学 / 植物 / 自他認識 / 分子生物学 / 園芸学 |
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| Outline of Final Research Achievements |
Self-incompatibility in Japanese pear is controlled by S-haplotype at the S-locus, which code a pistil S determinant, S-RNase, and pollen S determinants, S-locus F-box brothers (PpSFBBs). In this study, we expanded BAC contigs around S-RNase to find at least 17 PpSFBB2s coded by S2-haplotype. PpSFBB2 homologs cloned from pollen of S homozygous lines had high identity between haplotypes, which predicts that the collaborative non-self S-RNase recognition model in Solanaceae species fits the S-RNase-based SI systems in the Japanese pear. Using the immune microscopy, we confirmed endocytic uptake of S-RNase into pollen tube, and subsequent trafficking via endosomes.
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Report
(5 results)
Research Products
(19 results)
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[Journal Article] Cloning an S-RNase allele, including the longest intron, from cultivars of European pear (Pyrus communis L.)2013
Author(s)
Takasaki-Yasuda, T., Nomura, N., Moriya, Y., Okada, K., Iwanami, H. and Bessho, H.
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Journal Title
J. Hortic. Sci. Biotech.
Volume: 88
Pages: 427-432
Related Report
Peer Reviewed
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[Journal Article] S-RNase-based genotyping of triploid cultivars of European pear (Pyrus communis L.)2013
Author(s)
Takasaki-Yasuda, T., Nomura, N., Moriya, Y., Okada, K., Iwanami, H. and Bessho, H.
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Journal Title
J. Hortic. Sci. Biotech.
Volume: 88
Pages: 751-755
Related Report
Peer Reviewed
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