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Development of methods that allows visualization of freezing behaviors in cold hardy plant tissues and determination of factors contributing to tissue freezing behaviors.

Research Project

Project/Area Number 23380023
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Horticulture/Landscape architecture
Research InstitutionNational Institute of Agrobiological Sciences

Principal Investigator

Ishikawa Masaya  国立研究開発法人農業生物資源研究所, その他部局等, 研究専門員 (90355727)

Research Collaborator MURAKAWA Hiroki  
KUCHITSU Kazuyuki  
TOYOMASU Takayuki  
IDE Hiroyuki  
ARATA Yoji  
YAMAZAKI Hideyuki  
FUKAMI Reiko  
KURIYAMA Akira  
NADA Hiroki  
ECHIGO Takuya  
Price William S.  
Project Period (FY) 2011-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥16,900,000 (Direct Cost: ¥13,000,000、Indirect Cost: ¥3,900,000)
Fiscal Year 2014: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2013: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2012: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2011: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
Keywords耐寒性 / 赤外線サーモビュア / 凍結 / 水 / 可視化 / MRI / 氷核活性 / 氷 / サーモグラフィ / 植物 / 果樹 / 非破壊可視化 / 凍結過程 / 冷凍顕微鏡 / SEM / 赤外線
Outline of Final Research Achievements

Diversity and mechanisms involved in freezing behaviors of cold hardy plant tissues remain unknown. To address this question, we attempted to develop non-invasive methods that allow visualization of freezing behaviors in plant tissues using MRI and infra-red thermography. These methods enabled to clarify tissue- and species-specific freezing behaviors (freeze autonomously or remain stably unfrozen) and the order of freezing among tissues. These traits were closely related to the distribution of ice nucleation activity (INA), the ability of cause heterogeneous ice nucleation, in the tissues. Tissue INA reached the maximal just before the first autumnal frost and likely functions as the freezing sensor that allows to induce freezing in appropriate places and timing.

Report

(5 results)
  • 2015 Final Research Report ( PDF )
  • 2014 Annual Research Report
  • 2013 Annual Research Report
  • 2012 Annual Research Report
  • 2011 Annual Research Report
  • Research Products

    (37 results)

All 2016 2015 2014 2013 2012 2011 Other

All Int'l Joint Research (1 results) Journal Article (13 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 13 results,  Open Access: 5 results,  Acknowledgement Compliant: 1 results) Presentation (22 results) (of which Invited: 6 results) Patent(Industrial Property Rights) (1 results)

  • [Int'l Joint Research] Nanoscale Organisation and Dynamics/Western Sydney University(オーストラリア)

    • Related Report
      2014 Annual Research Report
  • [Journal Article] Freezing behaviors in wintering Cornus florida flower bud tissues revisited using MRI2016

    • Author(s)
      Ishikawa M, Ide H, Yamazaki H, Murakawa H, Kuchitsu K, Price WS, Arata Y
    • Journal Title

      Plant Cell Envrionment

      Volume: ?

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Ice nucleation activity in various tissues of Rhododendron flower buds: their relevance to extraorgan freezing.2015

    • Author(s)
      Ishikawa M, Ishikawa M, Toyomasu T, Aoki T, Price WS
    • Journal Title

      Frontiers in Plant Science- Functional Plant Ecology

      Volume: 6 Pages: 149-149

    • DOI

      10.3389/fpls.2015.00149

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Factors contributing to deep supercooling capability and cold survival in dwarf bamboo (Sasa senanensis) leaf blades.2015

    • Author(s)
      Ishikawa M., Oda A, Fukami R, Kuriyama A.
    • Journal Title

      Frontiers in Plant Science (Functional Plant Ecology)

      Volume: 5

    • DOI

      10.3389/fpls.2014.00791

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] 植物組織の氷核活性の検索2014

    • Author(s)
      石川雅也
    • Journal Title

      低温生物工学会誌

      Volume: 60 Pages: 79-88

    • NAID

      110009878790

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] OsATG7 is required for autophagy-dependent lipid metabolism in rice postmeiotic anther development2014

    • Author(s)
      Takamitsu Kurusu, Tomoko Koyano, Shigeru Hanamata, Takahiko Kubo, Yuhei Noguchi, Chikako Yagi, Noriko Nagata, Takashi Yamamoto, Takayuki Ohnishi, Yozo Okazaki, Nobutaka Kitahata, Daichi Ando, Masaya Ishikawa, Shinya Wada, Akio Miyao, Hirohiko Hirochika, Hiroaki Shimada, Amane Makino, Kazuki Saito, Hiroyuki Ishida, Tetsu Kjnoshita, Nori Kurata and Kazuyuki Kuchitsu^*
    • Journal Title

      Autophagy

      Volume: 10(5) Issue: 5 Pages: 878-888

    • DOI

      10.4161/auto.28279

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] High ice nucleation activity located in blueberry stem bark is linked to primary freeze initiation and adaptive freezing behavior of the bark.2014

    • Author(s)
      Kishimoto T, Yamazaki H, Saruwatari A, Murakawa H, Sekozawa Y, Kuchitsu K, Price WS, Ishikawa M.
    • Journal Title

      AoB PLANTS

      Volume: 6 Issue: 0 Pages: plu044-plu044

    • DOI

      10.1093/aobpla/plu044

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Seasonal changes in ice nucleation activity in blueberry stems and effects of cold treatments in vitro2014

    • Author(s)
      Kishimoto T, Sekozawa Y, Yamazaki H, Murakawa H, Kuchitsu K, Ishikawa M
    • Journal Title

      Environmental and Experimental Botany

      Volume: ? Pages: 13-23

    • DOI

      10.1016/j.envexpbot.2014.02.010

    • Related Report
      2013 Annual Research Report 2012 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] OsATG7 is required for autophagy-dependent lipid metabolism in rice postmeiotic anther development2014

    • Author(s)
      Kurusu T, Koyano T, Hanamata S, Kubo T, Noguchi Y, Yagi C, Nagata N, Yamamoto T, Ohnishi T, Okazaki Y, Kitahata N, Ando D, Ishikawa M, Wada S, Miyao A, Hirochika H, Shimada H, Makino A, Saito K, Ishida H, Kinoshita T, Kurata N, Kuchitsu K
    • Journal Title

      Autophagy

      Volume: 10

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Comparison of long-term up-regulated genes during induction of freezing tolerance by cold and ABA in bromegrass cell cultures revealed by microarray analyses2013

    • Author(s)
      Nakamura T, Yazaki J, Kishimoto N, Kikuchi S, Robertson A.J, Gusta L.V, Ishikawa M
    • Journal Title

      Plant Growth Regulation

      Volume: 71(2): Issue: 2 Pages: 113-136

    • DOI

      10.1007/s10725-013-9812-9

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Abscisic acid induced freezing tolerance in chilling-sensitive suspension cultures and seedlings of rice2013

    • Author(s)
      Shinkawa R, Morishita A, Amikura K, Machida R, Murakawa H, Kuchitsu K, Ishikawa M
    • Journal Title

      BMC Research Notes

      Volume: 6 Issue: 1 Pages: 35-35

    • DOI

      10.1186/1756-0500-6-351

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] ブルーベリーの冬の枝の凍結過程を可視化する2012

    • Author(s)
      山崎秀幸、石川雅也(石川雅也)
    • Journal Title

      画像ラボ

      Volume: 2 Pages: 64-47

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] チシマザサ葉身及び葉鞘における凍結挙動の解析2011

    • Author(s)
      深見怜子、山本浩二、奥島里美、栗山 昭、石川雅也(石川雅也)
    • Journal Title

      低温生物工学会誌

      Volume: 57 Pages: 71-76

    • NAID

      110008671125

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 示差赤外線サーモグラフィーおよび示差熱分析によるブルーベリー枝の凍結挙動の解析2011

    • Author(s)
      山崎秀幸、吉田慎吾、石川雅也(石川雅也)
    • Journal Title

      低温生物工学会誌

      Volume: 57 Pages: 77-81

    • NAID

      110008671126

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Presentation] 耐寒性植物組織にみられる凍結制御に関わる構造と機能2016

    • Author(s)
      石川雅也
    • Organizer
      第61 回低温生物工学会大会セミナー:「細胞・組織の凍結及び乾燥耐性機構の解明とその応用」
    • Place of Presentation
      東京電機大・鳩山キャンパス
    • Year and Date
      2016-06-25
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] ワサビ種子の成熟と長期保存の検討2016

    • Author(s)
      馬場冨二夫、本間義之、石川雅也
    • Organizer
      第61 回低温生物工学会大会
    • Place of Presentation
      東京電機大・鳩山キャンパス
    • Year and Date
      2016-06-25
    • Related Report
      2014 Annual Research Report
  • [Presentation] MRI による凍結様式可視化解析法の有効性:アメリカハナミズキ越冬花芽の再解析2016

    • Author(s)
      石川 雅也,井出 博之,山崎 秀幸,村川 裕基,朽津 和幸,Price William,荒田 洋治
    • Organizer
      日本植物生理学会
    • Place of Presentation
      岩手大学
    • Year and Date
      2016-03-18
    • Related Report
      2014 Annual Research Report
  • [Presentation] Preferential freezing avoidance localized in the anthers and embryo sacs in wintering flower buds of Daphne kamtschatica var. jezoensis revealed by NMR micro-imaging.2015

    • Author(s)
      Ishikawa M, Ide H, Tsujii T, Price, WS, Arata Y.
    • Organizer
      Bio-imaging学会
    • Place of Presentation
      東京理科大(葛飾キャンパス)
    • Year and Date
      2015-09-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] 26 年間超低温保存したワサビ種子の発芽2015

    • Author(s)
      本間義之、馬場富二夫、中野敬之、石川雅也
    • Organizer
      園芸学会
    • Place of Presentation
      徳島大学常三島キャンパス
    • Year and Date
      2015-09-26
    • Related Report
      2014 Annual Research Report
  • [Presentation] レンギョウ枝髄に含まれる新規氷核活性物質の活性機構2015

    • Author(s)
      灘浩樹,越後拓也,豊増孝之,山崎秀幸,村川裕基,朽津和幸,石川雅也
    • Organizer
      第60回低温生物工学会
    • Place of Presentation
      東京工科大学(八王子キャンパス)
    • Year and Date
      2015-05-30
    • Related Report
      2014 Annual Research Report
  • [Presentation] レンギョウ枝髄の氷核活性物質の単離同定2014

    • Author(s)
      石川雅也、村川裕基、朽津和幸、灘浩樹、越後拓也
    • Organizer
      日本植物学会第78回大会
    • Place of Presentation
      明治大学 生田
    • Year and Date
      2014-09-12 – 2014-09-14
    • Related Report
      2013 Annual Research Report
  • [Presentation] シュウ酸カルシウム一水和物結晶表面における氷核生成促進メカニズム2014

    • Author(s)
      灘浩樹,石川雅也,越後拓也
    • Organizer
      日本物理学会秋季大会
    • Place of Presentation
      中部大学
    • Year and Date
      2014-09-07 – 2014-09-10
    • Related Report
      2013 Annual Research Report
  • [Presentation] Ice nucleation activity in plants: from the survey to isolation, identification, characterization of responsible substances.2014

    • Author(s)
      Ishikawa M.
    • Organizer
      10th International Plant Cold Hardiness Seminar (invited speaker)
    • Place of Presentation
      Poznan Poland
    • Year and Date
      2014-08-17 – 2014-08-21
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Visualization of freezing behaviors in cold hardy plant tissues using high resolution MRI and Infra-red thermography.2014

    • Author(s)
      Masaya Ishikawa, Hideyuki Yamazaki, William S. Price
    • Organizer
      10th International Plant Cold Hardiness Seminar, (student course invited lecture)
    • Place of Presentation
      Poznan Poland
    • Year and Date
      2014-08-16 – 2014-08-18
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] シナレンギョウ枝髄に含まれる新規氷核活性物質と枝の凍結過程2014

    • Author(s)
      豊増孝之、山崎秀幸、村川裕基、朽津和幸、石川雅也
    • Organizer
      第59回低温生物工学会
    • Place of Presentation
      九州大学
    • Year and Date
      2014-06-28 – 2014-06-29
    • Related Report
      2013 Annual Research Report
  • [Presentation] シュウ酸カルシウム一水和物(100)面上での氷核生成機構:分子動力学シミュレーション研究2014

    • Author(s)
      灘 浩樹、石川雅也、越後 拓也
    • Organizer
      日本地球惑星科学連合2014大会
    • Place of Presentation
      横浜パシフィコ
    • Year and Date
      2014-04-28 – 2014-05-02
    • Related Report
      2013 Annual Research Report
  • [Presentation] 植物組織の氷核活性の検索2013

    • Author(s)
      27.石川雅也
    • Organizer
      第58回低温生物工学会セミナー・年会
    • Place of Presentation
      関西大学
    • Related Report
      2012 Annual Research Report
  • [Presentation] ブルーベリー枝の氷核活性の機能・役割の解析2013

    • Author(s)
      山崎秀幸, 石川雅也, 中谷容子
    • Organizer
      第58回低温生物工学会セミナー・年会
    • Place of Presentation
      関西大学
    • Related Report
      2012 Annual Research Report
  • [Presentation] 器官外凍結と凍結制御2013

    • Author(s)
      石川雅也
    • Organizer
      日本植物学会第77回大会
    • Place of Presentation
      北海道大学
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] シナレンギョウ枝の凍結挙動と氷核活性2013

    • Author(s)
      豊増孝之, 山崎秀幸, 石川雅也
    • Organizer
      日本植物学会第77回大会
    • Place of Presentation
      北海道大学
    • Related Report
      2012 Annual Research Report
  • [Presentation] ブルーベリー枝の氷核活性の分布と役割2013

    • Author(s)
      山崎秀幸, 村川裕基, 朽津和幸, 石川雅也
    • Organizer
      日本植物学会第77回大会
    • Place of Presentation
      北海道大学
    • Related Report
      2012 Annual Research Report
  • [Presentation] Freezing process visualized using infra-red thermography and factors involved in freeze-regulation in wintering woody stems.2012

    • Author(s)
      Ishikawa M, Yamazaki H (Ishikawa M)
    • Organizer
      Plant and Microbe Adaptations to Cold 2012
    • Place of Presentation
      Sapporo
    • Related Report
      2011 Annual Research Report
  • [Presentation] 耐寒性植物組織における氷晶析出と凍結制御機構2012

    • Author(s)
      石川雅也
    • Organizer
      雪氷研究大会 凍土・雪氷物性合同分科会
    • Place of Presentation
      福山
    • Related Report
      2011 Annual Research Report
    • Invited
  • [Presentation] Effect of abscisic acid on freezing behavior in rice seedlings2012

    • Author(s)
      Murakawa H, Kuchitsu K, Ishikawa M (Ishikawa M)
    • Organizer
      Plant and Microbe Adaptations to Cold 2012
    • Place of Presentation
      Sapporo
    • Related Report
      2011 Annual Research Report
  • [Presentation] Visualization of freezing process and elucidation of factors involved in freeze-regulation in wintering tissues2011

    • Author(s)
      Ishikawa M, Yamazaki H, Fukami R (Ishikawa M)
    • Organizer
      9th International Plant Cold Hardiness Seminar
    • Place of Presentation
      Luxemburg,
    • Related Report
      2011 Annual Research Report
    • Invited
  • [Presentation] ブルーベリー枝凍結挙動の可視化解析2011

    • Author(s)
      山﨑秀幸, 石川雅也 (石川雅也)
    • Organizer
      日本バイオイメージング学会
    • Place of Presentation
      千歳
    • Related Report
      2011 Annual Research Report
  • [Patent(Industrial Property Rights)] シュウ酸カルシウムを含む氷核活性剤2015

    • Inventor(s)
      石川雅也、野村孝之、槌谷明日香
    • Industrial Property Rights Holder
      石川雅也、野村孝之、槌谷明日香
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2012-202489
    • Filing Date
      2015-07-14
    • Related Report
      2014 Annual Research Report

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Published: 2011-04-06   Modified: 2019-07-29  

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