Identification and characterization of victorin-binding protein in oat plants
Project/Area Number |
23380026
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Plant pathology
|
Research Institution | Kagawa University |
Principal Investigator |
TADA Yasuomi 香川大学, 総合生命科学研究センター, 准教授 (40552740)
|
Co-Investigator(Kenkyū-buntansha) |
AKIMITSU Kazuya 香川大学, 農学部, 教授 (80263888)
|
Project Period (FY) |
2011-04-01 – 2014-03-31
|
Project Status |
Completed (Fiscal Year 2013)
|
Budget Amount *help |
¥18,850,000 (Direct Cost: ¥14,500,000、Indirect Cost: ¥4,350,000)
Fiscal Year 2013: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2012: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2011: ¥8,320,000 (Direct Cost: ¥6,400,000、Indirect Cost: ¥1,920,000)
|
Keywords | 感染生理 / 植物病理学 / 宿主特異的毒素 / 植物免疫応答 / ビクトリン / 特異性決定因子 / エンバク / エンバク葉枯病菌 |
Research Abstract |
To identify victorin-binding proteins, a crude protein was extracted from the cell wall of the rice plant. The extract was fractionated by a gel filtration chromatography. Then, we have performed Biacore analysis to test if victorin binds to a cell wall protein. The Biacore positive fraction was mixed with the biotin-conjugated victorin (VicBio) and pulled down the victorin-binding proteins. A 15 kDa protein was specifically detected in VicBio-treated fraction, and mass spectrometry identified it as thioredoxin known as a reducing enzyme.
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Report
(4 results)
Research Products
(21 results)
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[Presentation] Identification of a novel victorin-binding protein in oats2013
Author(s)
Kaneichi, D., Itaya, T., Shimakawa, K., Oka, N., Nomoto, M., Akimitsu, K., and Tada, Y
Organizer
Phytogene Symposium VI
Place of Presentation
香川
Year and Date
2013-10-28
Related Report
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[Presentation] ビクトリンと相互作用する新奇タンパク質の同定2013
Author(s)
嶋川今日子, 兼市大輝, 劉 小露, 板谷知健, 内橋幸平, 齋藤隆一郎, 日下 広, 中屋敷均, 土佐幸雄, 大谷耕平, 眞山滋志, 秋光和也, 多田安臣
Organizer
平成25年度日本植物病理学会大会
Place of Presentation
岐阜
Year and Date
2013-03-28
Related Report
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[Presentation] Identification of a novel victorin-binding protein in oats2013
Author(s)
Kaneichi, D., Shimakawa, K., Itaya, T., Oka, N., Nomoto, M., Akimitsu, K. Tada, Y.
Organizer
農学先端国際フォーラム『ファイトジーンの可能性と未来VI』
Place of Presentation
香川県、香川国際会議場
Related Report
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