Identification of putative plant defense activators of novel mode of action.
Project/Area Number |
23380027
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Plant pathology
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Research Institution | Tokyo University of Science |
Principal Investigator |
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Co-Investigator(Renkei-kenkyūsha) |
KURUSU Takamitsu 東京工科大学, 応用生物学部, 助教 (50422499)
KAYA Hidetaka 農業生物資源研究所, 研究員 (80398825)
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Project Period (FY) |
2011-04-01 – 2014-03-31
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Project Status |
Completed (Fiscal Year 2013)
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Budget Amount *help |
¥19,240,000 (Direct Cost: ¥14,800,000、Indirect Cost: ¥4,440,000)
Fiscal Year 2013: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2012: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2011: ¥8,060,000 (Direct Cost: ¥6,200,000、Indirect Cost: ¥1,860,000)
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Keywords | 膜交通系 / 活性酸素種 / カルシウムイオン / 植物免疫 / 植物防除剤 / 植物免疫活性化剤 / 抵抗性誘導剤 / ケミカルバイオロジー / 陰イオンチャネル / サリチル酸 / ジャスモン酸 / オートファジー / シグナル伝達系 / サリチル酸経路 / 植物防疫剤 / ジャスモン酸経路 / 植物免役 / タバコ培養細胞BY-2 / エリシター / カルシウムチャネル / ファイトアレキシン / 植物免役活性化剤 |
Research Abstract |
Plant defense activators are chemicals that induce disease resistance by enhancing plant immunity upon pathogen infection. Defense signaling pathways induced by cryptogein, a proteinaceous elicitor derived from a pathogenic oomycete, in tobacco BY-2 cells has been extensively studied as a simple model system. Based on the correlation between the temporal pattern of enzymatic production of reactive oxygen species (ROS) and downstream defense responses, we established a new high-throughput screening method to identify novel lead compounds for plant defense activators. From a total of 11,000 compounds screened, we selected 58 candidates for activators. We further examined the effects of selected compounds on the expression of defense-related genes in Arabidopsis plants, and discovered some chemicals that potentially enhance jasmonic acid-dependent signaling pathway as putative plant defense activators with a novel mode of action.
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Report
(4 results)
Research Products
(234 results)
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[Journal Article] Ca^<2+>-activated reactive oxygen species production by Arabidopsis RbohH and RbohJ is essential for proper pollen tube tip growth2014
Author(s)
Kaya, H., Nakajima, R., Iwano, M., Kanaoka, M., Kimura, S., Takeda, S., Kawarazaki, T., Senzaki, E., Hamamura, H., Higashiyama, T., Takayama, S., Abe, M., and Kuchitsu, K.
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Journal Title
Plant Cell
Volume: 26 (3)
Issue: 3
Pages: 1069-1080
DOI
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Peer Reviewed
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[Journal Article] OsATG7 is required for autophagy-dependent lipid metabolism in rice post-meiotic anther development2014
Author(s)
Kurusu T, Koyano T, Hanamata S, Kubo T, Noguchi Y, Y agi C, Nagata N, Y amamoto T, Ohnishi T, Okazaki Y, Kitahata N, Ando D, Ishikawa M, Wada S, Miyao A, Hirochika H, Shimada S, Makino A, Saito K, Ishida H, Kinoshita T, Kurata N, Kuchitsu K
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Journal Title
Autophagy
Volume: 10
Pages: 860-870
URL
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Peer Reviewed
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[Journal Article] An S-Type Anion Channel SLAC1 Is Involved in Cryptogein-Induced Ion Fluxes and Modulates Hypersensitive Responses in Tobacco BY-2 Cells2013
Author(s)
Kurusu, T., Saito, K., Horikoshi, S., Hanamata, S., Negi, J., Yagi, C., Kitahata, N., Iba, K. and Kuchitsu, K.
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Journal Title
PLOS One
Volume: 8
Issue: 8
Pages: e70623-e70623
DOI
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Peer Reviewed / Open Access
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[Journal Article] Coordination structures of Mg^<2+> and Ca^<2+> in three types of tobacco calmodulins in solution: Fourier-transform infrared spectroscopic studies of side-chain coo- groups2013
Author(s)
N. Suzuki, L. F. Imai, Y. Kato, K. Nagata, Y. Ohashi, K. Kuchitsu, M. Tanokura, A. Sakamoto, M. Nara, M. Nakano, and N. Yonezawa
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Journal Title
Biopolymers
Volume: 99
Issue: 7
Pages: 472-483
DOI
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Peer Reviewed
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[Journal Article] Involvement of the putative Ca^<2+>- permeable mechanosensitive channels, NtMCA1 and NtMCA2, in Ca2+ uptake, Ca2^<+>-dependent cell proliferation and mechanical stress-induced gene expression in tobacco (Nicotiana tabacum) BY-2 cells2011
Author(s)
Kurusu T, Yamanaka T, Nakano M, Takiguchi A, Ogasawara Y, Hayashi T, Iida K, Hanamata S, Shinozaki K, Iida H, Kuchitsu K
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Journal Title
Journal of Plant Research
Volume: 13(印刷中)
Issue: 4
Pages: 555-568
DOI
NAID
Related Report
Peer Reviewed
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[Journal Article] Cryptogein-induced cell cycle arrest at G2 phase is associated with inhibition of cyclin-dependent kinases, suppression of expression of cell cycle-related genes and protein degradation in synchronized tobacco BY-2 cells2011
Author(s)
Ohno, R., Kadota, Y., Fujii, S., Sekine, M., Umeda, M., Kuchitsu, K.
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Journal Title
PLANT AND CELL PHYSIOLOGY
Volume: 52
Issue: 5
Pages: 922-932
DOI
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Peer Reviewed
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[Presentation] OsATG7 is required for autophagy-dependent lipid metabolism in rice postmeiotic anther development.2014
Author(s)
Kurusu T,Koyano T,Hanamata S,Kubo T,Noguchi Y,Yagi C,Nagata N,Yamamoto T,Ohnishi T,Okazaki Y,Kitahata N,Ando D,Ishikawa M,Wada S,Miyao A,Hirochika H,Shimada H,Makino A,Saito K,Ishida H,Kinoshita T,Kurata N,Kuchitsu K
Organizer
UK-Japan Workshop "Rhizosphere Interactions-towards Global Food Security -"
Place of Presentation
理化学研究所横浜キャンパス
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[Presentation] イネの雄性生殖器官発達におけるオートファジーの役割2013
Author(s)
小谷野 智子, 来須 孝光, 花俣 繁, 久保 貴彦, 野口 祐平, 八木 智華子, 永田 典子,大西 孝幸, 木下 哲, 倉田 のり, 朽津 和幸
Organizer
日本植物生理学会
Place of Presentation
岡山大学
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[Presentation] Roles of autophagy in male reproductive development and fertilization in rice.2012
Author(s)
Tomoko Koyano, Takamitsu Kurusu, Shigeru Hanamata, Takahiko Kubo, Yuhei Noguchi, Chikako Yagi, Noriko Nagata, Takayuki Ohnishi, Tetsu Kinoshita, Nori Kurata, Kazuyuki Kuchitsu
Organizer
International Symposium on the Mechanisms of Sexual Reproduction in Animals and Plants.
Place of Presentation
名古屋市
Related Report
Invited
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[Presentation] Regulation and physiological roles of NADPH oxidase-mediated production of reactive oxygen species in angiosperms and bryophytes.2012
Author(s)
S. Yabuta, T. Yamada, S. Kimura, T. Kawarazaki, A. Iizuka, H. Kaya, T. Kurus, K. Ishizaki, R. Nishihama, T. Kohchi, K. Kuchitsu
Organizer
International Marchantia Workshop 2012
Place of Presentation
ホテルグリーンピア南阿蘇
Related Report
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[Presentation] 植物の生殖・受精におけるオートファジーの新たな役割2012
Author(s)
来須孝光, 小谷野智子, 花俣繁久保, 貴彦, 永田典子, 野口祐平, 大西孝幸, 宮尾安藝, 雄木下哲, 倉田のり, 朽津和幸
Organizer
シンポジウム「細胞間相互認識とアロ認証」
Place of Presentation
筑波大学
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[Presentation] イネ培養細胞のタンパク質性エリシター誘導性感染防御応答制御におけるCa2+透過チャネルOsTPC1の機能2012
Author(s)
波田晴康, 能鹿島央司, 清塚正弘, 来須孝光, 大熊英治, 小谷野智子, 岡田憲典, 古賀仁一郎, 山根久和, 村田芳行, 朽津和幸
Organizer
第53回日本植物生理学会年会
Place of Presentation
京都産業大学
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[Book] Plant signaling networks involving reactive oxygen species and Ca^<2+> ., Handbook on Reactive Oxygen Species (ROS): Formation Mechanisms, Physiological Roles and Common Harmful Effects., Suzuki M, Yamamoto S (Eds)2013
Author(s)
Kurusu T, Kimura S, T ada Y , Kaya H, Kuchitsu K
Publisher
Nova Science Publishers, Inc. NY , USA
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