Degradation of rRNA in insect cells antiviral immune response
| Project/Area Number |
23380033
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied entomology
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| Research Institution | Nagoya University |
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Principal Investigator |
IKEDA Motoko 名古屋大学, 生命農学研究科, 教授 (20262892)
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥19,500,000 (Direct Cost: ¥15,000,000、Indirect Cost: ¥4,500,000)
Fiscal Year 2013: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2012: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2011: ¥11,830,000 (Direct Cost: ¥9,100,000、Indirect Cost: ¥2,730,000)
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| Keywords | 核多角体病ウイルス / 昆虫細胞 / 抗ウイルス応答 / リボソームRNA分解 / 全タンパク質合成停止 / カイコ / 昆虫培養細胞 / エンドリボクヌレアーゼ / エンドリボクヌレアーゼ阻害因子 / P143 / 宿主特異性 / 昆虫ウイルス |
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| Research Abstract |
Previously, we demonstrated that rRNA degradation occurs in Bombyx cells (BM-N cells) during abortive infection with heterologous nucleopolyhedroviruses (NPVs). rRNA degradation is likely linked to the global protein synthesis shutdown observed in BM-N cells infected with heterologous NPVs. In the present study, we examined the molecular mechanisms of rRNA degradation of BM-N cells. We cloned cDNAs of RNase and RNase inhibitor from BM-N cells possibly responsible for rRNA degradation, and functional analyses were performed by the transient gene expression and RNAi-based gene silencing. We also examined the possible Bombyx mori NPV factor(s) responsible for inhibition of rRNA degradation in BM-N cells.
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Report
(4 results)
Research Products
(79 results)
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[Journal Article] Degradation of ribosomal RNA in BM-N cells from the silkworm Bombyx mori during abortive infection with heterologous nucleopolyhedroviruses2013
Author(s)
Hamajima, R., Ito, Y., Ichikawa, H., Mitsutake, H., Kobyashi, J., Kobayashi, M. and Ikeda, M
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Journal Title
J. Gen. Virol
Volume: 94
Issue: 9
Pages: 2102-2111
DOI
Related Report
Peer Reviewed
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[Journal Article] Chondroitinase from baculovirus Bombyx mori nucleopolyhedrovirus and chondroitin sulfate from silkworm Bombyx mori.2013
Author(s)
Sugiura, N., Ikeda, M., Shioiri, T., Yoshimura, M., Kobayashi, M. and Watanabe, H.
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Journal Title
Glycobiology
Volume: 23
Issue: 12
Pages: 1520-1530
DOI
Related Report
Peer Reviewed
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