Molecular mechanisms for apoptosis induction and suppression n insect cells infected with nucleopolyhedroviruses
Project/Area Number |
23380034
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Applied entomology
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Research Institution | Nagoya University |
Principal Investigator |
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Project Period (FY) |
2011-04-01 – 2014-03-31
|
Project Status |
Completed (Fiscal Year 2013)
|
Budget Amount *help |
¥16,770,000 (Direct Cost: ¥12,900,000、Indirect Cost: ¥3,870,000)
Fiscal Year 2013: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2012: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2011: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
|
Keywords | アポトーシス / Apsup / Dronc / カスパーゼ / バキュロウイルス / 核多角体病ウイルス / カイコ / マイマイガ / caspase / P53 / Sir2 / イニシエーターカスパーゼ / Dronc相同体 / 一過性発現 / IAP / NPV / 昆虫 |
Research Abstract |
Apoptosis serves as the major anti-viral defense mechanism in baculovirus-infected lepidopteran insect cells. In the present study, we examined molecular mechanisms underlying induction and inhibition of apoptosis that occur in insect cells upon infection with nucleopolyhedroviruses (NPVs). We cloned a number of cDNAs of cellular and viral factors, which possibly function in the apoptotic pathways, from several NPVs and insect cells. These include cellular factors Dronc, Caspase-1s, IAPs, P53, Reaper and Sirt2, and viral factors Apsup and IAPs. Through functional analysis of these factors in transient expression experiments, we provide several important insights into regulation mechanisms of apoptosis induction and inhibition in NPV-infected insect cells. We also demonstrated that IAP1 inhibits Dronc activation and promotes degradation of activated Dronc to prevent apoptosis induction in non-apoptotic insect cells.
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Report
(4 results)
Research Products
(72 results)
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[Journal Article] Degradation of ribosomal RNA in BM-N cells from the silkworm Bombyx mori during abortive infection with heterologous nucleopolyhedroviruses2013
Author(s)
Hamajima, R., Ito, Y., Ichikawa, H., Mitsutake, H., Kobyashi, J., Kobayashi, M. and Ikeda, M
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Journal Title
J. Gen. Virol
Volume: 94
Issue: 9
Pages: 2102-2111
DOI
Related Report
Peer Reviewed
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[Journal Article] Chondroitinase from baculovirus Bombyx mori nucleopolyhedrovirus and chondroitin sulfate from silkworm Bombyx mori.2013
Author(s)
Sugiura, N., Ikeda, M., Shioiri, T., Yoshimura, M., Kobayashi, M. and Watanabe, H.
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Journal Title
Glycobiology
Volume: 23
Issue: 12
Pages: 1520-1530
DOI
Related Report
Peer Reviewed
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[Journal Article] Cloning and characterization of a Dronc homologue in the silkworm, Bombyx mori2011
Author(s)
Suganuma, I., Ushiyama, T., Yamada, H., Iwamato, A., Kobayashi, M, Ikeda, M.
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Journal Title
Insect Biochemistry and Molecular Biology
Volume: 41
Pages: 909-921
Related Report
Peer Reviewed
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