Study on the insect male reproduction molecules mechanism from the viewpoint of the sperm-activating factor.
| Project/Area Number |
23380036
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied entomology
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| Research Institution | Kyoto Institute of Technology |
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Principal Investigator |
NAGAOKA SUMIHARU 京都工芸繊維大学, 工芸科学研究科, 助教 (00303933)
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| Co-Investigator(Kenkyū-buntansha) |
SHIRAI Koji 信州大学, 繊維学部, 准教授 (00293499)
YAMAMOTO Koji 九州大学, 農学研究科, 助教 (00346834)
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥19,630,000 (Direct Cost: ¥15,100,000、Indirect Cost: ¥4,530,000)
Fiscal Year 2013: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2012: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2011: ¥12,350,000 (Direct Cost: ¥9,500,000、Indirect Cost: ¥2,850,000)
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| Keywords | 昆虫 / カイコガ / 生殖 / 精子成熟 / プロテアーゼ / 酵素 / 生理活性 / プロテオーム / 昆虫精子 / 精子活性化因子 / セリンプロテアーゼ / ペプチダーゼ / 酸素 / 発生・分化 |
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| Research Abstract |
A serine protease called initiatorin (BmIni) is required for sperm activation in the silkmoth, Bombyx mori. It has been expected that BmIni is responsible for the cleavage of seminal and/or sperm proteins, mainly at Arg-Arg sites, and thus is involved in the sperm activation. However, activation of sperm motility has been little known to other insects. (1) To determine the molecular mechanism of signaling pathways for sperm motility, we examined the evidence that BmIni acts on carboxypeptidase B (BmCPB). (2) We found a crucial role for BmIni-like serine protease activity in initiating conserved signaling pathways for sperm activation in many Lepidoptera, Orthoptera, Hemiptera species.
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Report
(4 results)
Research Products
(48 results)
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[Journal Article] Identification and characterization of a feeding-modulating peptide, hemolymph major anionic peptide (HemaP) from the sweetpotato hornworm, Agrius convolvuli2011
Author(s)
Morooka, N., Nagata, S., Shirai, K., Kiguchi, K., Nagasawa, H.
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Journal Title
FEBS Journal
Volume: 279
Pages: 168-179
Related Report
Peer Reviewed
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[Journal Article] Central projections of the wing afferents in the hawkmoth, Agrius convolvuli2011
Author(s)
Ando, N., Wang, H., Shirai, K., Kiguchi, K., Kanzaki, R.
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Journal Title
Journal of Insect Physiology
Volume: 57
Pages: 1518-1536
Related Report
Peer Reviewed
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[Journal Article] Molecular characterization of an insecticide-induced novel glutathione transferase in silkworm2011
Author(s)
Yamamoto, K., Ichinose, H., Aso, Y., Banno, Y., Kimura, M., Nakashima, T.
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Journal Title
Biochimica et Biophysica Acta
Volume: 1810
Pages: 420-426
Related Report
Peer Reviewed
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[Journal Article] Crystallographic survey of active sites of an unclassified glutathione transferase from Bombyx mori2011
Author(s)
Katuta, Y., Usuda, K., Nakashima, T., Kimura, M., Aso, Y., Yamamoto, K.
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Journal Title
Biochimica et Biophysica Acta
Volume: 1810
Pages: 1355-1360
Related Report
Peer Reviewed
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[Journal Article] Characterization of an omega-class glutathione S-transferase in the stress response of the silkmoth2011
Author(s)
Yamamoto, K., Teshiba, S., Shigeoka, Y., Aso, Y., Bannno, Y., Katakura, Y.
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Journal Title
Insect Molecular Biology
Volume: 20
Pages: 379-386
Related Report
Peer Reviewed
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