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Transcription profiles of host plant genes induced in the rhizobial and arbuscular mycorrhizal symbioses of soybean root

Research Project

Project/Area Number 23380042
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Plant nutrition/Soil science
Research InstitutionChiba University

Principal Investigator

SAKAMOTO Kazunori  千葉大学, 園芸学研究科, 教授 (10225807)

Co-Investigator(Renkei-kenkyūsha) MATSUI Akihiro  独立行政法人理化学研究所, 環境資源科学研究センター, 特別研究員 (90443027)
Project Period (FY) 2011-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000)
Fiscal Year 2013: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2012: ¥8,580,000 (Direct Cost: ¥6,600,000、Indirect Cost: ¥1,980,000)
Fiscal Year 2011: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Keywordsダイズ / 根粒菌 / アーバスキュラー菌根菌 / 共生関係 / 遺伝子発現 / DNAマイクロアレイ / 遺伝子 / 植物 / 土壌学 / 微生物 / マイクロアレイ
Research Abstract

In this study, the transcription profiles of host genes induced by rhizobial, arbuscular mycorrhizal and dual inoculations were analyzed to elucidate the host genes involving in the establishment of the dual symbiosis in soybean root. We found that 34 genes of host plant were induced commonly by rhizobial, mycorrhizal and dual inoculations, suggesting that these genes are essential for establishing dual symbiosis. The gene expression of type1 metallothionein and CPRD49 were examined using real-time RT-PCR. It was ascertained that the expression of type1 metallothionein gene was significantly induced in the root nodule and mycorrhizal root of soybean. We tried to examine the spatial expression patterns of type1 metallothionein gene in the transgenic hairy roots using promoter-gus gene fusions.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Annual Research Report
  • 2011 Annual Research Report
  • Research Products

    (26 results)

All 2013 2012 2011 Other

All Journal Article (8 results) (of which Peer Reviewed: 8 results) Presentation (15 results) Book (1 results) Remarks (2 results)

  • [Journal Article] Involvement of autoregulation in the interaction between rhizobial nodulation and AM fungal colonization in soybean roots2013

    • Author(s)
      Sakamoto, K., Ogiwara, N., and Kaji, T
    • Journal Title

      Biology and Fertility of Soils

      Volume: 49(8) Issue: 8 Pages: 1141-1152

    • DOI

      10.1007/s00374-013-0804-8

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Laccase-catalyzed oxidation of iodide and formation of organically bound iodine in soils2013

    • Author(s)
      Seki, M., Oikawa, J., Taguchi, T., Ohnuki, T., Muramatsu, Y., Sakamoto, K., and Amachi, S.
    • Journal Title

      Environmental Science and Technology

      Volume: 47 Issue: 1 Pages: 390-397

    • DOI

      10.1021/es303228n

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Arabidopsis non-coding RNA regulation in abiotic stress responses (Review)2013

    • Author(s)
      Matsui, A. , Nguyen, A.H. , Nakaminami, K. , Seki, M.
    • Journal Title

      International Journal of Molecular Sciences

      Volume: 14 Issue: 11 Pages: 22642-22654

    • DOI

      10.3390/ijms141122642

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Complete Genome Sequence of <i>Bradyrhizobium</i> sp. S23321: Insights into Symbiosis Evolution in Soil Oligotrophs2012

    • Author(s)
      大久保 卓
    • Journal Title

      Microbes and Environments

      Volume: 27 Issue: 3 Pages: 306-315

    • DOI

      10.1264/jsme2.ME11321

    • NAID

      10030869620

    • ISSN
      1342-6311, 1347-4405
    • Related Report
      2013 Final Research Report 2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 屋上緑化薄層基盤軽量土壌の経年変化および栄養塩類の推移2012

    • Author(s)
      黒沼尊紀
    • Journal Title

      日本緑化工学会誌

      Volume: 38 Pages: 51-55

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Phylogenetic relationships among non-pathogenic isolates of dark septate endophytes from Ericaceae plants2011

    • Author(s)
      Vano, I., Sakamoto, K., and Inubushi, K
    • Journal Title

      HortResearch

      Volume: 65 Pages: 41-47

    • NAID

      40018926278

    • Related Report
      2013 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Biocontrol efficiency of Fusarium wilt diseases by a root colonizing fungus Penicillium sp2011

    • Author(s)
      Alam, S.S., Sakamoto, K., and Inubushi, K
    • Journal Title

      Soil Science and Plant Nutrition

      Volume: 57 Issue: 2 Pages: 204-212

    • DOI

      10.1080/00380768.2011.564996

    • NAID

      110008897278

    • Related Report
      2013 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effect of Penicillium sp.EU0013 inoculation on tomato growth and Fusarium wilt2011

    • Author(s)
      Alam, S.S., Sakamoto, K., Inubushi, K.
    • Journal Title

      HortResearch

      Volume: 65 Pages: 69-73

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Presentation] ダイズの根粒・菌根共生系におけるType1 メタロチオネイン遺伝子の発現2013

    • Author(s)
      坂本一憲・上野 満・福田智代・青柳美里 ・ 園田雅俊 ・ 渡辺正巳 ・ 西野栄正
    • Organizer
      菌根研究会2013年度大会
    • Place of Presentation
      東北大学川渡共同セミナーセンター (大崎市)
    • Year and Date
      2013-11-16
    • Related Report
      2013 Final Research Report
  • [Presentation] Transcription profiles of host plant genes induced by rhizobial, AM fungal and dual inoculations in soybean root2013

    • Author(s)
      Sakamoto, K., Ogiwara, N., Kaji, T., Seki, M., Matsui, A., Ishida, J. and Tanaka, M
    • Organizer
      18th International Congress on Nitrogen Fixation
    • Place of Presentation
      Phoenix Seagaia Resort (Miyazaki city)
    • Year and Date
      2013-10-16
    • Related Report
      2013 Final Research Report
  • [Presentation] ダイズの根粒共生系と菌根共生系で発現する宿主遺伝子の網羅的解析 (第 3 報) ダイズの野生型と根粒超着生変異体との比較2013

    • Author(s)
      坂本一憲・梶 智光・荻原菜津子・杉本百合恵 ・ 関 原明 ・ 松井章浩 ・ 石田順子・田中真帆
    • Organizer
      日本土壌肥料学会名古屋大会
    • Place of Presentation
      名古屋大学(名古屋市)
    • Year and Date
      2013-09-13
    • Related Report
      2013 Final Research Report
  • [Presentation] ダイズの根粒共生系と菌根共生系で発現する宿主遺伝子の網羅的解析 (第 2 報) 単独共生と二重共生の比較2012

    • Author(s)
      坂本一憲・荻原菜津子・梶 智光・杉本百合恵 ・ 関 原明 ・ 松井章浩 ・ 石田順子・田中真帆
    • Organizer
      日本土壌肥料学会鳥取大会
    • Place of Presentation
      鳥取大学(鳥取市)
    • Year and Date
      2012-09-04
    • Related Report
      2013 Final Research Report
  • [Presentation] 亜鉛添加および共生微生物の感染がダイズのメタロチオネイン遺伝子の発現に及ぼす影響2012

    • Author(s)
      上野満・杉本百合恵・坂本一憲・園田雅俊
    • Organizer
      日本土壌肥料学会鳥取大会
    • Place of Presentation
      鳥取大学(鳥取市)
    • Year and Date
      2012-09-04
    • Related Report
      2013 Final Research Report
  • [Presentation] ダイズの根粒共生系と菌根共生系で発現する宿主遺伝子の網羅的解析(第2報)単独共生と二重共生の比較2012

    • Author(s)
      坂本一憲
    • Organizer
      日本土壌肥料学会
    • Place of Presentation
      鳥取大学(鳥取市)
    • Related Report
      2012 Annual Research Report
  • [Presentation] 亜鉛添加および共生微生物の感染がダイズのメタロチオネイン遺伝子の発現に及ぼす影響2012

    • Author(s)
      上野満
    • Organizer
      日本土壌肥料学会
    • Place of Presentation
      鳥取大学(鳥取市)
    • Related Report
      2012 Annual Research Report
  • [Presentation] リアルタイム PCR 法を用いたダイズの根粒および菌根共生系で高発現する宿主遺伝子の定量解析2011

    • Author(s)
      杉本百合恵 ・ 坂本一憲 ・ 園田雅俊
    • Organizer
      日本土壌肥料学会つくば大会
    • Place of Presentation
      つくば国際会議場(つくば市)
    • Year and Date
      2011-08-08
    • Related Report
      2013 Final Research Report
  • [Presentation] アーバスキュラー菌根共生系と根粒共生系の共通性と特異性, 日本土壌肥料学会シンポジウム 「アーバスキュラー菌根菌 : 研究の最前線と土壌肥料分野への貢献」2011

    • Author(s)
      坂本一憲
    • Organizer
      日本土壌肥料学会つくば大会
    • Place of Presentation
      つくば国際会議場(つくば市)
    • Year and Date
      2011-08-08
    • Related Report
      2013 Final Research Report
  • [Presentation] アーバスキュラー菌根共生系と根粒共生系の共通性と特異性2011

    • Author(s)
      坂本一憲
    • Organizer
      日本土壌肥料学会シンポジウム「アーバスキュラー菌根菌:研究の最前線と土壌肥料分野への貢献」
    • Place of Presentation
      つくば国際会議場(つくば市)(招待講演)
    • Year and Date
      2011-08-08
    • Related Report
      2011 Annual Research Report
  • [Presentation] リアルタイムPCR法を用いたダイズの根粒および菌根共生系で高発現する宿主遺伝子の定量解析2011

    • Author(s)
      杉本百合恵・坂本一憲・園田雅俊
    • Organizer
      日本土壌肥料学会
    • Place of Presentation
      つくば国際会議場(つくば市)
    • Year and Date
      2011-08-08
    • Related Report
      2011 Annual Research Report
  • [Presentation] ダイズの根粒着生と菌根形成の相互作用に対するオートレギュレーション機構の関与

    • Author(s)
      坂本一憲 ・ 荻原菜津子 ・ 梶 智光
    • Organizer
      植物微生物研究会第21回研究交流会
    • Place of Presentation
      岡山大学(岡山市)
    • Related Report
      2013 Final Research Report
  • [Presentation] ダイズの根粒共生系と菌根共生系で発現する宿主遺伝子の網羅的解析(第3報)ダイズの野生型と根粒超着生変異体との比較

    • Author(s)
      坂本一憲・梶 智光・荻原菜津子・杉本百合恵・関 原明・松井章浩・石田順子・田中真帆
    • Organizer
      日本土壌肥料学会2013年度名古屋大会
    • Place of Presentation
      名古屋大学(愛知県名古屋市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Transcription profiles of host plant genes induced by rhizobial, AM fungal and dual inoculations in soybean root

    • Author(s)
      Sakamoto, K., Ogiwara, N., Kaji, T., Seki, M., Matsui, A., Ishida, J. and Tanaka, M.
    • Organizer
      18th International Congress on Nitrogen Fixation
    • Place of Presentation
      フェニックス・シーガイア・リゾート(宮崎県宮崎市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] ダイズの根粒・菌根共生系におけるType1メタロチオネイン遺伝子の発現

    • Author(s)
      坂本一憲・上野 満・福田智代・青柳美里・園田雅俊・渡辺正巳・西野栄正
    • Organizer
      菌根研究会2013年度大会
    • Place of Presentation
      東北大学川渡共同セミナーセンター(宮城県大崎市鳴子温泉)
    • Related Report
      2013 Annual Research Report
  • [Book] 地下水用語集2011

    • Author(s)
      坂本一憲(共同執筆)
    • Total Pages
      143
    • Publisher
      理工図書
    • Related Report
      2011 Annual Research Report
  • [Remarks] 千葉大学・植物栄養学研究グループ・ホームページ

    • URL

      https://sites.google.com/site/plantnlab/

    • Related Report
      2013 Final Research Report
  • [Remarks] 千葉大学・植物栄養学研究グループ ホームページ

    • URL

      https://sites.google.com/site/plantnlab/

    • Related Report
      2013 Annual Research Report

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Published: 2011-04-06   Modified: 2019-07-29  

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