Transcription profiles of host plant genes induced in the rhizobial and arbuscular mycorrhizal symbioses of soybean root
| Project/Area Number |
23380042
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Plant nutrition/Soil science
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| Research Institution | Chiba University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
MATSUI Akihiro 独立行政法人理化学研究所, 環境資源科学研究センター, 特別研究員 (90443027)
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000)
Fiscal Year 2013: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2012: ¥8,580,000 (Direct Cost: ¥6,600,000、Indirect Cost: ¥1,980,000)
Fiscal Year 2011: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
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| Keywords | ダイズ / 根粒菌 / アーバスキュラー菌根菌 / 共生関係 / 遺伝子発現 / DNAマイクロアレイ / 遺伝子 / 植物 / 土壌学 / 微生物 / マイクロアレイ |
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| Research Abstract |
In this study, the transcription profiles of host genes induced by rhizobial, arbuscular mycorrhizal and dual inoculations were analyzed to elucidate the host genes involving in the establishment of the dual symbiosis in soybean root. We found that 34 genes of host plant were induced commonly by rhizobial, mycorrhizal and dual inoculations, suggesting that these genes are essential for establishing dual symbiosis. The gene expression of type1 metallothionein and CPRD49 were examined using real-time RT-PCR. It was ascertained that the expression of type1 metallothionein gene was significantly induced in the root nodule and mycorrhizal root of soybean. We tried to examine the spatial expression patterns of type1 metallothionein gene in the transgenic hairy roots using promoter-gus gene fusions.
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Report
(4 results)
Research Products
(26 results)
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[Journal Article] Laccase-catalyzed oxidation of iodide and formation of organically bound iodine in soils2013
Author(s)
Seki, M., Oikawa, J., Taguchi, T., Ohnuki, T., Muramatsu, Y., Sakamoto, K., and Amachi, S.
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Journal Title
Environmental Science and Technology
Volume: 47
Issue: 1
Pages: 390-397
DOI
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Peer Reviewed
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[Presentation] Transcription profiles of host plant genes induced by rhizobial, AM fungal and dual inoculations in soybean root2013
Author(s)
Sakamoto, K., Ogiwara, N., Kaji, T., Seki, M., Matsui, A., Ishida, J. and Tanaka, M
Organizer
18th International Congress on Nitrogen Fixation
Place of Presentation
Phoenix Seagaia Resort (Miyazaki city)
Year and Date
2013-10-16
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[Presentation] Transcription profiles of host plant genes induced by rhizobial, AM fungal and dual inoculations in soybean root
Author(s)
Sakamoto, K., Ogiwara, N., Kaji, T., Seki, M., Matsui, A., Ishida, J. and Tanaka, M.
Organizer
18th International Congress on Nitrogen Fixation
Place of Presentation
フェニックス・シーガイア・リゾート（宮崎県宮崎市）
Related Report
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