Arsenic transport system by plant-soil microorganism interaction
Project/Area Number |
23380044
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Plant nutrition/Soil science
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Research Institution | National Institute for Agro-Environmental Sciences |
Principal Investigator |
ISHIKAWA Satoru 独立行政法人農業環境技術研究所, その他部局等, 研究員 (40354005)
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Co-Investigator(Kenkyū-buntansha) |
TAKAGI Kazuhiro (独)農業環境技術研究所, 土壌環境研究領域, 上席研究員 (70354074)
FUJIMAKI Shu (独)日本原子力研究開発機構, 量子ビーム応用研究部門, 研究グループリーダー (20354962)
SUZUI Nobuo (独)日本原子力研究開発機構, 量子ビーム応用研究部門, 研究副主幹 (20391287)
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Project Period (FY) |
2011-04-01 – 2014-03-31
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Project Status |
Completed (Fiscal Year 2013)
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Budget Amount *help |
¥18,980,000 (Direct Cost: ¥14,600,000、Indirect Cost: ¥4,380,000)
Fiscal Year 2013: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2011: ¥8,060,000 (Direct Cost: ¥6,200,000、Indirect Cost: ¥1,860,000)
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Keywords | 無機ヒ素 / メチルヒ素 / イネ根圏 / 土壌微生物 / ヒ素 / メチルヒ素化合物 / 水稲 / 微生物 / 遺伝子 / 土壌細菌 / イネ |
Research Abstract |
We isolated from the rice rhizosphere two bacteria responsible for As methylation. Strain GSRB54 and GSRB05 were classified into the genus Streptomyces and Burkholderia, respectively, by 16S rRNA sequencing. The arsenite S-adenosylmethionine methyltransferase (arsM) gene of GSRB54 was phylogenetically different from known arsM genes in other bacteria. Heterologous expression of GSRB54 arsM in E. coli converted the methylation of As(III) into DMA. On the other hand, strain GSRB05 produced an unknown organic As compound (uAs1). The analyses by using LC/MS/MS, 1H, 13C-NMR and 2D-NMR elucidated that the chemical structure of uAs1 is amino acid derivative with a monomethylated As partial structure and we named it as Arsenothricin (AST). AST was degraded into MMA by Arthrobacter sp. These results suggest that the bacteria isolated from this study are associated with methylated As accumulation in rice grains through As methylation in the rice rhizosphere.
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Report
(4 results)
Research Products
(17 results)
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[Presentation] Arsenic Dynamics in Rhizosphere and Relevance to Arsenic Speciation in Rice Grains - Arsenic Transformation by Rhizobacteria Separated from Rice Plant-2013
Author(s)
Masato Kuramata, Ryota Kataoka, Tadashi Abe, Ken-ichi Yamazaki, Futa Sakakibara, Maki Asano, Koji Baba, Kazuhiro Takagi, Shuntaro Hiradate, Tsunashi Kamo, Satoru Ishikawa
Organizer
国際植物栄養科学会議
Place of Presentation
トルコ イスタンブール
Related Report
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[Presentation] イネ根圏土壌から分離した微生物のヒ素代謝2012
Author(s)
倉俣正人, 片岡良太, 山崎健一, 榊原風太, 馬場浩司, 石坂眞澄, 安部匡, 髙木和広, 加茂綱嗣, 平舘俊太郎, 石川覚
Organizer
ヒ素シンポジウム
Place of Presentation
宮崎大学
Related Report
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[Presentation] Arsenic Dynamics in Rhizosphere and Relevance to Arsenic Speciation in Rice Grains - Arsenic Transformation by Rhizobacteria Separated from Rice Plant-
Author(s)
Kuramata M, Kataoka R, Abe T, Yamazaki K, Sakakibara F, Asano M, Baba K, Takagi K, Hiradate S, Kamo T, Ishikawa S
Organizer
XVII. INTERNATIONAL PLANT NUTRITION COLLOQUIUM
Related Report
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[Presentation] イネ根圏微生物が生産する新規ヒ素化合物の同定
Author(s)
倉俣正人, 片岡良太, 山崎健一, 榊原風太, 馬場浩司, 石坂眞澄, 安部匡, 高木和広, 加茂綱嗣, 平舘俊太郎, 石川覚
Organizer
第19回ヒ素シンポジウム講演要旨集
Related Report
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[Presentation] イネ根圏土壌から分離した微生物のヒ素代謝
Author(s)
倉俣正人, 片岡良太, 山崎健一, 榊原風太, 馬場浩司, 石坂眞澄, 安部匡, 高木和広, 加茂綱嗣, 平舘俊太郎, 石川覚
Organizer
第18回ヒ素シンポジウム講演要旨集
Related Report
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