| Budget Amount *help |
¥18,720,000 (Direct Cost: ¥14,400,000、Indirect Cost: ¥4,320,000)
Fiscal Year 2014: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2013: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2012: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2011: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
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| Outline of Final Research Achievements |
tmRNA, which functions as both tRNA and mRNA, is invoved in an abnormal translation process, trans-translation, to rescue stalled ribosomes in eubacterial cells. We have developed an in vitro system to investigate the molecular mechanism of trans-translation using purified necessary components for the reaction. Using this system, we determined the relative positions of tRNA/smpB on the ribosome in each steps of trans-translation process by hydroxyl-radical probing experiment and FLET measurements. We also indicated that tmRNA/SmpB/EF-Tu/GTP complex recognizes the ribosomal state whether the 3'end of mRNA remains on the A-site of ribosome, and when the 3'end of mRNA remains on A-site, the complex releases ribosomes after GTP hydrolysis.
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