Molecular mechanism and physiological roles of trans-translation
| Project/Area Number |
23380045
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Applied microbiology
|
|---|
| Research Institution | Hirosaki University |
|---|
Principal Investigator |
AKIRA Muto 弘前大学, 農学生命科学部, 研究員 (80034635)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
HIMENO Hyouta 弘前大学, 農学生命科学部, 教授 (80208785)
|
|---|
| Project Period (FY) |
2011-04-01 – 2015-03-31
|
| Project Status |
Completed (Fiscal Year 2014)
|
| Budget Amount *help |
¥18,720,000 (Direct Cost: ¥14,400,000、Indirect Cost: ¥4,320,000)
Fiscal Year 2014: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2013: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2012: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2011: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
|
|---|
| Keywords | trans-translation / tmRNA / tRNA / mRNA / ribosome / EF-Tu / SmpB / ribosome resque / トランス翻訳 / リボソーム / 大腸菌 / 枯草菌 / translation / bacteria / SerRS / 翻訳停滞解消機構 / SerS |
|---|
| Outline of Final Research Achievements |
tmRNA, which functions as both tRNA and mRNA, is invoved in an abnormal translation process, trans-translation, to rescue stalled ribosomes in eubacterial cells. We have developed an in vitro system to investigate the molecular mechanism of trans-translation using purified necessary components for the reaction. Using this system, we determined the relative positions of tRNA/smpB on the ribosome in each steps of trans-translation process by hydroxyl-radical probing experiment and FLET measurements. We also indicated that tmRNA/SmpB/EF-Tu/GTP complex recognizes the ribosomal state whether the 3'end of mRNA remains on the A-site of ribosome, and when the 3'end of mRNA remains on A-site, the complex releases ribosomes after GTP hydrolysis.
|
Report
(5 results)
Research Products
(43 results)
-
[Journal Article] Structural insights into the assembly of the 30S ribosomal subunit in vivo: functional role of S5 and location of the 17S rRNA precursor sequence.2014
Author(s)
Yang, Z., Guo, Q., Goto, S., Chen, Y., Li, N., Yan, K., Zhang, Y., Muto, A., Deng, H., Himeno, H., Lei, J. & Gao, N.:
-
Journal Title
Protein Cell,
Volume: 5
Pages: 394-407
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
-
-
-
-
-
-
-
[Journal Article] Dissecting the in vivo assembly of the 30S ribosomal subunit reveals the role of RimM and general features of the assembly process.2013
Author(s)
Guo, Q., Goto, S., Chen, Y., Feng, B., Xu, Y., Muto, A., Himeno, H., Deng, H., Lei, J., Gao, N.
-
Journal Title
Nucleic Acids Res.
Volume: 41
Issue: 4
Pages: 2609-2620
DOI
Related Report
Peer Reviewed
-
[Journal Article] Rice Annotation Project Database (RAP-DB): an integrative and interactive database for rice genomics2013
Author(s)
Sakai H, Lee SS, Tanaka T, Numa H, Kim J, Kawahara Y, Wakimoto H, Yang CC, Iwamoto M, Abe T, Yamada Y, Muto A, Inokuchi H, Ikemura T, Matsumoto T, Sasaki T, Itoh T.
-
Journal Title
Plant Cell Physiol.
Volume: 52
Issue: 2
Pages: e6-e6
DOI
Related Report
Peer Reviewed
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
[Presentation] tRNADB-CE 2011 : tRNA gene database curated manually by experts2011
Author(s)
Abe, T., Ikemura, T., Sugahara, J., Kanai, A., Ohara, Y., Uehara, M., Kinouchi, M., Kanaya, S., Yamada, Y., Muto, A., Inokuchi, H
Organizer
RNA2011 (The 16^<th> Annual Meeting of the RNA Society)
Place of Presentation
Kyoto
Related Report
-
-
-
-
-
-
-
-
-
-
-
