Studies on the novel cellular functions of phospholipases A2

• Project/Area Number: 23380047
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied microbiology
• Research Institution: The University of Tokyo
• Principal Investigator: ARIOKA Manabu 東京大学, 農学生命科学研究科, 准教授 (20242159)
• Project Period (FY): 2011-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥19,110,000 (Direct Cost: ¥14,700,000、Indirect Cost: ¥4,410,000); Fiscal Year 2013: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2012: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2011: ¥10,010,000 (Direct Cost: ¥7,700,000、Indirect Cost: ¥2,310,000)
• Keywords: phospholipase A2 / lysophosphatidylcholine / Aspergillus oryzae / nerve growth factor / MAPK / ホスホリパーゼA2 / リン脂質 / ミトコンドリア / 神経成長因子 / MAPキナーゼ / 生存・分化

Research Abstract

(1) One of the secretory phospholipases A2 (PLA2s) in Aspergillus oryzae, sPlaB, displayed PLA1, not PLA2, activity.
(2) A cytosolic PLA2-like protein in A. oryzae, AoPlaA, displayed phosphatidylethanolamine-specific PLA2 activity. Alanine substitution of putative active-site residues resulted in the loss of PLA2 activity.
(3) Nerve growth factor (NGF)-induced mitogen-activated protein kinase (MAPK) phosphorylation in PC12 cells was found to be potentiated by the addition of lysophosphatidylcholine (LPC). Group X PLA2 displayed an effect similar to LPC. LPC augmented NGF-induced activation of TrkA, the receptor for NGF, thereby potentiating the downstream signaling pathways. By domain-swapping experiments, the extracellular domain of NGF was shown to be responsible for the effect of LPC.

Research Products

• [Journal Article] Lysophosphatidylcholine potentiates BDNF-induced TrkB phosphorylation and downstream signals in cerebellar granule neurons Biosci (2013)
  • Author(s): Wuhanqimuge and Arioka, M
  • Journal Title: Biotechnol. Biochem Volume: 77 Pages: 2510-2513
  • URL: https://www.jstage.jst.go.jp/article/bbb/77/12/77_130622/_article
• [Journal Article] Lysophosphatidylcholine enhances NGF-induced MAPK and Akt signals through the extracellular domain of TrkA in PC12 cells (2013)
  • Author(s): Wuhanqimuge, Itakura, A., Matsuki, Y ., T anaka, M., and Arioka, M
  • Journal Title: FEBS Open Bio Volume: 3 Issue: 1 Pages: 243-251
  • DOI: 10.1016/j.fob.2013.05.003
  • Peer Reviewed
• [Journal Article] Functional analysis of Abp1p-interacting proteins involved in endocytosis of the MCC component in A spergillus oryzae (2013)
  • Author(s): Matsuo, K., Higuchi, Y ., Kikuma, T ., Arioka, M., and Kitamoto, K
  • Journal Title: Fungal Genet. Biol Volume: 56 Pages: 125-134
  • DOI: 10.1016/j.fgb.2013.03.007
  • Peer Reviewed
• [Journal Article] Lysophosphatidylcholine potentiates BDNF-induced TrkB phosphorylation and downstream signals in cerebellar granule neurons. (2013)
  • Author(s): Wuhanqimuge, Arioka M.
  • Journal Title: Biosci Biotechnol Biochem Volume: 77 Pages: 2510-2513
  • NAID: 130003381974
  • Peer Reviewed
• [Journal Article] A novel glucose-tolerant b-glucosidase from the salivary gland of the termite Nasutitermes takasagoensis. (2013)
  • Author(s): Uchima, C.A.
  • Journal Title: J. Gen. Appl. Microbiol. Volume: in press
  • Peer Reviewed
• [Journal Article] Heterologous Expression and Characterization of a Glycoside Hydrolase Family 45 endo-b-1,4-Glucanase from a Symbiotic Protist of the Lower Termite, Reticulitermes speratus. (2013)
  • Author(s): Otagiri, M.
  • Journal Title: Appl. Biochem. Biotechnol. Volume: 169 Issue: 6 Pages: 1910-1918
  • DOI: 10.1007/s12010-012-9992-1
  • Peer Reviewed
• [Journal Article] Heterologous expression in Pichia pastoris and characterization of an endogenous thermostable and high-glucose-tolerant b-glucosidase from the termite Nasutitermes takasagoensis. (2012)
  • Author(s): Uchima, C.A.
  • Journal Title: Appl. Environ. Microbiol. Volume: 78 Issue: 12 Pages: 4288-4293
  • DOI: 10.1128/aem.07718-11
  • Peer Reviewed
• [Journal Article] Expression and one-step purification of recombinant proteins using an alternative episomal vector for the expression of N-tagged heterologous proteins in Pichia pastoris. (2012)
  • Author(s): Uchima, C.A.
  • Journal Title: Biosci. Biotechnol. Biochem. Volume: 76 Pages: 368-371
  • Peer Reviewed
• [Journal Article] Cellulolytic environment in the midgut of the wood-feeding higher termite Nasutitermes takasagoensis (2012)
  • Author(s): Tokuda, G., Watanabe, H., Hojo, M., Fujita, A., Makiya, H., Miyagi, M., Arakawa, G., Arioka, M
  • Journal Title: Journal of Insect Physiology Volume: 58 Issue: 1 Pages: 147-154
  • DOI: 10.1016/j.jinsphys.2011.10.012
  • Peer Reviewed
• [Journal Article] A carrier fusion significantly induces unfolded protein response in heterologous protein production by A spergillus oryzae (2011)
  • Author(s): Ohno, A., Maruyama, J., Nemoto, T ., Arioka, M., and Kitamoto, K
  • Journal Title: Appl. Microbiol. Biotechnol Volume: 92 Issue: 6 Pages: 1197-1206
  • DOI: 10.1007/s00253-011-3487-9
  • Peer Reviewed
• [Journal Article] Functional analysis of the putative AAA A TPase AipA localizing at the endocytic sites in the filamentous fungus A spergillus oryzae (2011)
  • Author(s): Higuchi, Y ., Arioka, M., and Kitamoto, K
  • Journal Title: FEMS Microbiol. Lett Volume: 320 Issue: 1 Pages: 63-71
  • DOI: 10.1111/j.1574-6968.2011.02288.x
  • Peer Reviewed
• [Journal Article] Autophagy delivers misfolded secretory proteins accumulated in endoplasmic reticulum to vacuoles in the filamentous fungus A spergillus oryzae (2011)
  • Author(s): Kimura, S., Maruyama, J., Kikuma, T ., Arioka, M., and Kitamoto, K
  • Journal Title: Biochem. Biophys. Res. Commun Volume: 406 Issue: 3 Pages: 464-470
  • DOI: 10.1016/j.bbrc.2011.02.075
  • Peer Reviewed
• [Journal Article] Heterologous expression and characterization of a glucose-stimulated β-glucosidase from the termite Neotermes koshunensis in Aspergillus oryzae. (2011)
  • Author(s): Uchima, C.A.
  • Journal Title: Appl. Microbiol. Biotechnol. Volume: 89 Issue: 6 Pages: 1761-1771
  • DOI: 10.1007/s00253-010-2963-y
  • Peer Reviewed
• [Journal Article] High-throughput recombinant gene expression systems in Pichia pastoris using newly developed plasmid vectors. (2011)
  • Author(s): Sasagawa, T.
  • Journal Title: Plasmid Volume: 65 Issue: 1 Pages: 65-69
  • DOI: 10.1016/j.plasmid.2010.08.004
  • Peer Reviewed
• [Presentation] 麹菌 A spergillus oryzae 由来分泌型および細胞質型ホスホリパーゼ A2 の持つユニークな酵素学的性質 (2014)
  • Author(s): 高柳 亜由美, 駒井 紀之, 宮川 拓也, 大塚 淳, 田之倉 優, 北本 勝ひこ, 有岡 学
  • Organizer: 第14回東京大学生命科学シンポジウム
  • Place of Presentation: 東京大学
  • Year and Date: 2014-04-26
• [Presentation] 分泌型ホスホリパーゼ A2 は神経栄養因子からのシグナルを増強する (2014)
  • Author(s): 畢 純, オハンチメグ, 北本 勝ひこ, 有岡 学
  • Organizer: 日本農芸化学会大会
  • Place of Presentation: 東京
• [Presentation] 分泌型ホスホリパーゼ A2 は神経栄養因子からのシグナルを増強する (2014)
  • Author(s): 畢 純、オハン チメグ、北本 勝ひこ、有岡 学
  • Organizer: 日本農芸化学会2014年度大会
  • Place of Presentation: 明治大学生田キャンパス
• [Presentation] 麹菌 A . Oryzae におけるエンドサイトーシス関連タンパク質 AipB, AipC, A ipD の解析 (2013)
  • Author(s): 松尾 賢人, 樋口 裕次郎, 菊間 隆志, 有岡 学, 北本 勝ひこ
  • Organizer: 日本農芸化学会大会
  • Place of Presentation: 仙台
• [Presentation] シロアリ腸内に共生する原生生物由来GH26 β-マンナナーゼの構造学的解析 (2013)
  • Author(s): 塚越 光
  • Organizer: 日本農芸化学会大会
  • Place of Presentation: 仙台
• [Presentation] 麹菌A. oryzaeにおけるエンドサイトーシス関連タンパク質AipB, AipC, AipDの解析 (2013)
  • Author(s): 松尾 賢人
  • Organizer: 日本農芸化学会大会
  • Place of Presentation: 仙台
• [Presentation] Synergistic Effect of Lysophosphatidylcholine on Nerve Growth Factor- induced Mitogen-activated Protein Kinase (MAPK) and Akt Signals through the Extracellular Domain of Receptor TrkA in PC12 Cells (2012)
  • Author(s): Wuhanqimuge, Katsuhiko Kitamoto, and Manabu Arioka
  • Organizer: 53rd International Conference on the Bioscience of Lipids
  • Place of Presentation: Banff, Canada
• [Presentation] 麹菌 A . Oryzaeにおける細胞質型ホスホリパーゼ A_2 相同遺伝子 A opla A の機能解析 (2012)
  • Author(s): 駒井 紀之, 小谷 昌平, 北本 勝ひこ, 有岡 学
  • Organizer: 日本農芸化学会大会
  • Place of Presentation: 京都
• [Presentation] Synergistic effect of lysophosphatidylcholine on NGF-induced survival signaling pathways through receptor TrkA in PC12 cells (2012)
  • Author(s): Wuhanqimuge, Katsuhiko Kitamoto, Manabu Arioka
  • Organizer: 日本農芸化学会大会
  • Place of Presentation: 京都
• [Presentation] シロアリ腸内共生原生生物由来GH26 β-マンナナーゼの分解特性の解析 (2012)
  • Author(s): 塚越 光
  • Organizer: 日本応用糖質科学会平成24年度大会
  • Place of Presentation: 東京
• [Presentation] A spergillus oryzaeにおけるエンドサイトーシス関連遺伝子 aipA , aipC, aipD の解析 (2011)
  • Author(s): 松尾 賢人, 樋口 裕次郎, 有岡 学, 北本 勝ひこ
  • Organizer: 第11回東京大学生命科学シンポジウム
  • Place of Presentation: 東京大学
  • Year and Date: 2011-06-04
• [Presentation] 麹菌 A. oryzae はマクロオートファジー依存的に核を液胞に取り込む (2011)
  • Author(s): 菊間 隆志, 正路 淳也, 有岡 学, 北本 勝ひこ
  • Organizer: 第11回東京大学生命科学シンポジウム
  • Place of Presentation: 東京大学
  • Year and Date: 2011-06-04
• [Presentation] Lysophosphatidylcholine synergistically enhances NGF-induced MAPK and Akt signaling pathways through the NGF receptor TrkA in PC12 cells (2011)
  • Author(s): Wuhanqimuge, Katsuhiko Kitamoto, Manabu Arioka
  • Organizer: 第11回東京大学生命科学シンポジウム
  • Place of Presentation: 東京大学
  • Year and Date: 2011-06-04
• [Presentation] Functional analyses of three endocytosis-related components in the filamentous fungus A spergillus oryzae (2011)
  • Author(s): Kento Matsuo, Y ujiro Higuchi, Manabu Arioka, Katsuhiko Kitamoto
  • Organizer: 4th Congress of European Microbiologists, FEMS2011
  • Place of Presentation: Geneva, Switzerland
• [Presentation] 麹菌A. oryzaeにおける細胞質型ホスホリパーゼA2相同遺伝子AoplaAの機能解析
  • Author(s): 駒井 紀之
  • Organizer: 日本農芸化学会大会
  • Place of Presentation: 京都女子大学（京都）
• [Presentation] ヤマトシロアリ後腸共生原生生物由来GHF26 β-マンナナーゼの発現および酵素学的性質の解析
  • Author(s): 塚越 光
  • Organizer: 日本農芸化学会大会
  • Place of Presentation: 京都女子大学（京都）
• [Presentation] Synergistic effect of lysophosphatidylcholine on NGF-induced survival signaling pathways through receptor TrkA in PC12 cells
  • Author(s): Wuhanqimuge
  • Organizer: 日本農芸化学会大会
  • Place of Presentation: 京都女子大学（京都）
• [Presentation] A comparative study on endogenous beta-glucosidases from termites
  • Author(s): Cristiane A. Uchima
  • Organizer: International Symposium on Digestive Systems in Termites
  • Place of Presentation: Nishihara, Japan
• [Presentation] Advances in the heterologous expression systems of biomass-degrading enzymes from termites and their symbionts
  • Author(s): Manabu Arioka
  • Organizer: International Symposium on Digestive Systems in Termites
  • Place of Presentation: Nishihara, Japan
  • Invited
• [Presentation] Cellulolytic enzymes of termites and their symbionts: production and characterization of key enzymes in the protein factory, Aspergillus oryzae
  • Author(s): Manabu Arioka
  • Organizer: International Union of Microbiological Societies 2011 Congress
  • Place of Presentation: Sapporo, Japan
  • Invited
• [Presentation] Functional analyses of three endocytosis-related components in the filamentous fungus Aspergillus oryzae.
  • Author(s): Kento Matsuo
  • Organizer: 4th Congress of European Microbiologists, FEMS2011
  • Place of Presentation: Geneva, Switzerland
• [Presentation] Aspergillus oryzaeにおけるエンドサイトーシス関連遺伝子aipA、aipC、aipDの解析
  • Author(s): 松尾 賢人
  • Organizer: 第11回東京大学生命科学シンポジウム
  • Place of Presentation: 東京大学（東京）
• [Presentation] 麹菌A. oryzaeはマクロオートファジー依存的に核を液胞に取り込む
  • Author(s): 菊間 隆志
  • Organizer: 第11回東京大学生命科学シンポジウム
  • Place of Presentation: 東京大学（東京）
• [Presentation] Lysophosphatidylcholine synergistically enhances NGF-induced MAPK and Akt signaling pathways through the NGF receptor TrkA in PC12 cells
  • Author(s): Wuhanqimuge
  • Organizer: 第11回東京大学生命科学シンポジウム
  • Place of Presentation: 東京大学（東京）
• [Presentation] A study on glucosidases from termites
  • Author(s): Cristiane Uchima Akemi
  • Organizer: 第11回東京大学生命科学シンポジウム
  • Place of Presentation: 東京大学（東京）
• [Book] 発酵・醸造食品の最新技術と機能性II. 「麹菌によるシロアリセルラーゼの生産」（共著） (2011)
  • Author(s): 有岡 学
  • Publisher: シーエムシー出版