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Comprehensive functional analysis of protein kinase C of filamentous fungi

Research Project

Project/Area Number 23380048
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied microbiology
Research InstitutionThe University of Tokyo

Principal Investigator

HORIUCHI Hiroyuki  東京大学, 農学生命科学研究科, 准教授 (00209280)

Project Period (FY) 2011-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥18,720,000 (Direct Cost: ¥14,400,000、Indirect Cost: ¥4,320,000)
Fiscal Year 2013: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2012: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2011: ¥8,840,000 (Direct Cost: ¥6,800,000、Indirect Cost: ¥2,040,000)
Keywordsバイオテクノロジー / 糸状菌 / PKC / 細胞壁 / 微生物
Outline of Final Research Achievements

The functions of protein kinase C (PKC) in filamentous fungi remain largely unknown. Aspergillus nidulans is one of the model filamentous fungus and there is a PKC-encoding gene, pkcA, in its genome. In our previous study, we showed that pkcA is essential for the growth of A. nidulans. In this study, to clarify the function of PkcA, we constructed pkcA-ts mutant in which the activity of PkcA was temperature sensitive and R429A mutant that produces an activated form of PkcA under the alcA promoter of A. nidulans. We performed transcriptome analysis using these mutants and identified genes whose expression changed in response to the changes of the PkcA activity. Genes related to cell wall synthesis, cell polarization, Ca2+ signal transduction, and a mycotoxin, sterigmatocystin, synthesis were included in these genes. We analyzed the regulation mechanisms of the gene expression by PkcA and clarified them.

Report

(4 results)
  • 2014 Final Research Report ( PDF )
  • 2013 Annual Research Report
  • 2012 Annual Research Report
  • 2011 Annual Research Report
  • Research Products

    (9 results)

All 2015 2014 2012 2011 Other

All Journal Article (3 results) (of which Peer Reviewed: 2 results,  Acknowledgement Compliant: 1 results) Presentation (5 results) Book (1 results)

  • [Journal Article] Protein kinase C regulates the expression of cell wall-related genes in an RlmA-dependent and independent manner in Aspergillus nidulans2015

    • Author(s)
      T. Katayama, A. Ohta, H. Horiuchi
    • Journal Title

      Biosci. Biotechnol. Biochem.

      Volume: 79 Issue: 2 Pages: 321-330

    • DOI

      10.1080/09168451.2014.973365

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] 糸状菌の形態形成過程におけるプロテインキナーゼCの機能2014

    • Author(s)
      片山琢也、堀内裕之
    • Journal Title

      化学と生物

      Volume: 52

    • Related Report
      2012 Annual Research Report
  • [Journal Article] Involvement of protein kinase C in the suppression of apoptosis and in polarity establishment in Aspergillus nidulans under conditions of heat stress.2012

    • Author(s)
      Katayama, T., Uchida, H., Ohta, A., Horiuchi, H.
    • Journal Title

      PLoS ONE

      Volume: 7 Issue: 11 Pages: e50503-e50503

    • DOI

      10.1371/journal.pone.0050503

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Presentation] Aspergillus nidulansにおけるプロテインキナーゼCによるカルシウム応答シグナル伝達経路制御機構の解析2014

    • Author(s)
      片山琢也、志波優、吉川博文、堀内裕之
    • Organizer
      糸状菌分子生物学コンファレンス
    • Place of Presentation
      東北大学
    • Year and Date
      2014-11-15 – 2014-11-16
    • Related Report
      2013 Annual Research Report
  • [Presentation] 糸状菌Aspergillus nidulansにおけるプロテインキナーゼC失活条件における網羅的転写解析2014

    • Author(s)
      片山琢也、志波優、吉川博文、堀内裕之
    • Organizer
      日本ゲノム微生物学会
    • Place of Presentation
      東京農業大学
    • Year and Date
      2014-03-07 – 2014-03-09
    • Related Report
      2013 Annual Research Report
  • [Presentation] Aspergillus nidulansにおけるプロテインキナーゼCによる細胞壁合成酵素遺伝子群の転写制御についての解析2012

    • Author(s)
      片山琢也、太田明徳、堀内裕之
    • Organizer
      第12回糸状菌分子生物学コンファレンス
    • Place of Presentation
      愛知県産業労働センター・ウインクあいち
    • Related Report
      2012 Annual Research Report
  • [Presentation] Aspergillus nidulans における高温条件下でのフロテインキナーセC によるアホトーシス誘導抑制機構の解析2011

    • Author(s)
      片山琢也
    • Organizer
      第11回糸状菌分子生物学コンファレンス
    • Place of Presentation
      東京
    • Related Report
      2011 Annual Research Report
  • [Presentation] Aspergillus nidulansにおけるプロテインキナーゼCによる細胞壁合成酵素遺伝子の転写制御

    • Author(s)
      片山琢也、志波優、吉川博文、太田明徳、堀内裕之
    • Organizer
      日本農芸化学会2013年度大会
    • Place of Presentation
      東北大学
    • Related Report
      2012 Annual Research Report
  • [Book] Protein kinase C of filamentous fungi and its roles in the stresses affecting hyphal morphogenesis and conidiation in "Stress Biology of Yeasts and Fungi: Applications for Industrial Brewing and Fermentation"2015

    • Author(s)
      H. Horiuchi, T. Katayama
    • Publisher
      Springer
    • Related Report
      2013 Annual Research Report

URL: 

Published: 2011-04-06   Modified: 2019-07-29  

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