| Project/Area Number |
23380049
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied microbiology
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| Research Institution | Setsunan University (2013)
Kyoto University (2011-2012) |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
HASHIMOTO Wataru 京都大学, 大学院・農学研究科, 准教授 (30273519)
MIKAMI Bunzo 京都大学, 大学院・農学研究科, 教授 (40135611)
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥18,200,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥4,200,000)
Fiscal Year 2013: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2012: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Fiscal Year 2011: ¥7,280,000 (Direct Cost: ¥5,600,000、Indirect Cost: ¥1,680,000)
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| Keywords | ABCトランスポーター / 酸性多糖輸送 / Sphingomonas属細菌 / Ｘ線結晶構造 / 輸送体分子進化 / X線結晶構造 / 基質結合タンパク質 / アルギン酸 / スフィンゴモナス属細菌 / X線結晶構造解析 / プロテオリポソーム再構成系 / ヘテロ多糖 / 高分子輸送 / アルギン酸結合タンパク質 / ABCタンパク質 |
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| Research Abstract |
The structure and function relationship of macromolecule(alginate)-specific ABC transporter (importer:AlgM1-AlgM2/AlgS-AlgS) of Sphingomonas sp, A1 was analyzed.The substrate-binding proteins (AlgQ) bind/reliese alginate by opening/closing the N-domein and C-domein. The subsite 1 in the AlgQ specifically binds mannuronate. Through the contact with alginate-bound AlgQ, the permeases (AlgM1 and AlgM2) constituting importer open, receive alginate from AlgQ, and then alginate is introduced into cytosol by using ATP-hydrolyzing energy, which is generated by the action of AlgS (ATPase). This ATP transporter was different from the ABC transporters responsible for the transport of low-molecular weight substrates in that the substrate-binding protein (AlgQ) forms the tunnel that allows the passage of alginate, a long chain acid polysaccharide.
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