Elucidation of transcription network of stringent control in Bacillus subtilis and its application

• Project/Area Number: 23380053
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied microbiology
• Research Institution: Fukuyama University
• Principal Investigator: FUJITA Yasutaro 福山大学, 生命工学部, 教授 (40115506)
• Co-Investigator(Kenkyū-buntansha): HIROOKA Kazutake 福山大学, 生命工学部, 准教授 (20389068)
• Project Period (FY): 2011-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥11,830,000 (Direct Cost: ¥9,100,000、Indirect Cost: ¥2,730,000); Fiscal Year 2013: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2012: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2011: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
• Keywords: 緊縮応答 / 枯草菌 / 発現制御 / 応用微生物 / ゲノム / 胞子形成 / 緊縮転写制御 / プリン合成

Research Abstract

In Bacillus subtilis, stringent response evokes a GTP decrease and an ATP increase. As a result, positive and negative stringent transcription regulation of stringent operons possessing adenine and guanine in their transcription initiation sites, occur, respectively. Among positive stringent genes are kinA and kinB which are necessary for sporulation initiation. Operation of positive stringent transcription of kinA and kinB was found to be prerequisite for sporulation initiation.
As an approach to the applied field, guanine at the transcription initiation site of the pts operon under negative stringent control was converted to adenine, so that glucose metabolism was enhanced in the stringent conditions to result in more than 5-fold increase of isoleucine production by ilv-leu operon involved in branched-chain amino acid synthesis which is subjected to positive stringent transcription control.

Research Products

• [Journal Article] Structural characterization of a ligand-bound form of Bacillus subtilis FadR involved in the regulation of fatty acid degradation (2014)
  • Author(s): Masahiro Fujihashi, Taiga Nakatani, Kazutake Hirooka, Hiroshi Matsuoka, Yasutaro Fujita, Kunio Miki
  • Journal Title: Proteins Volume: (in press) Issue: 7 Pages: 1301-1310
  • DOI: 10.1002/prot.24496
• [Journal Article] Expression of kinA and kinB of Bacillus] subtilis, necessary for sporulation initiation, is under positive stringent transcription control. (2013)
  • Author(s): Tojo,S.,Hirooka,K.,and Fujita,Y.
  • Journal Title: Journal of Bacteriology Volume: 195 Issue: 8 Pages: 1656-1665
  • DOI: 10.1128/jb.02131-12
  • Peer Reviewed
• [Journal Article] Direct and indirect regulation of the ycnKJI operon involved in copper uptake through two transcriptional repressors, YcnK and CsoR, in Bacillus subtilis. (2012)
  • Author(s): Hirooka,K.,Edahiro,T.,Kimura,K.
  • Journal Title: Journal of Bacteriology Volume: 194 Issue: 20 Pages: 5675-5687
  • DOI: 10.1128/jb.00919-12
  • Peer Reviewed
• [Journal Article] 枯草菌Bacillus subtilis の胞子形成トリガーへの正の緊縮転写制御の関与 (2012)
  • Author(s): 東條繁郎, 広岡和丈, 藤田泰太郎
  • Journal Title: 福山大学生命工学部研究年報 Volume: 11 Pages: 1-20
  • URL: http://ci.nii.ac.jp/naid/110009465203
• [Journal Article] 枯草菌Bacillus subtilisの胞子形成トリガーへの正の緊縮転写制御の 関与 (2012)
  • Author(s): 東條繁郎、藤田泰太郎
  • Journal Title: 福山大学生命工学部研究年報 Volume: (11) Pages: 1-20
• [Journal Article] Identification of Aromatic Residues Critical to the DNA Binding and Ligand Response of the Bacillus subtilis QdoR (YxaF) Repressor Antagonized by Flavonoids (2011)
  • Author(s): Hirooka, K., and Fujita, Y.
  • Journal Title: Bioscience, Biotechnology, and Biochemistry Volume: 75 Issue: 7 Pages: 1325-1334
  • DOI: 10.1271/bbb.110098
  • NAID: 10029329273
  • ISSN: 0916-8451, 1347-6947
  • Peer Reviewed
• [Journal Article] Catabolite repression of the Bacillus subtilis FadR regulon, which is involved in fatty acid catabolism. (2011)
  • Author(s): Tojo, S., Satomura, T., Matsuoka, H., Hirooka, K., and Fujita Y.
  • Journal Title: Journal of Bacteriology Volume: 193 Issue: 10 Pages: 2388-2395
  • DOI: 10.1128/jb.00016-11
  • Peer Reviewed
• [Presentation] 枯草菌でのラムノース資化に関わるyuxG-yulBCDE オペロンにコードされる各タンパク質の機能解析 (2014)
  • Author(s): 広岡和丈, 藤田泰太郎
  • Organizer: 日本農芸化学会2014年度大会
  • Place of Presentation: 明治大学生田キャンパス, 東京都
  • Year and Date: 2014-03-28
• [Presentation] 枯草菌でのラムノ ース資化に関わるyuxG-yulBCDEオペロンにコードされる各タンパク質の機能解析 (2014)
  • Author(s): 広岡和丈、藤田泰太郎
  • Organizer: 日本農芸化学会2014年度大会
  • Place of Presentation: 明治大学生田キャンパス、東京都
• [Presentation] 枯草菌の炭素代謝と胞子形成開始の緊縮転写制御 (2013)
  • Author(s): 藤田泰太郎, 東條繁郎, 広岡和丈
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド, 神戸市
  • Year and Date: 2013-12-03
• [Presentation] 枯草菌におけるラムノース異化に関わるyuxG-yulBCDE オペロンのYulB とCcpA による制御機構 (2013)
  • Author(s): 広岡和丈, 藤田泰太郎
  • Organizer: 2013年度グラム陽性菌ゲノム機能会議
  • Place of Presentation: 筑波山ホテル江戸屋, つくば市
  • Year and Date: 2013-09-07
• [Presentation] Stringent transcription control of carbon metabolism and sporulation initiation in Bacillus subtilis (2013)
  • Author(s): Yasutaro Fujita, Shigeo Tojo, Kazutake Hirooka
  • Organizer: 7th International Conference on Gram-Positive Microorganisms
  • Place of Presentation: Palazzo dei Congressi, Montecatini Terme, Italy
  • Year and Date: 2013-06-25
  • Invited
• [Presentation] 枯草菌におけるラムノース異化に関わる遺伝子群の発現制御機構の解析 (2013)
  • Author(s): 広岡和丈, 枝廣貴成, 木村晃輔, 藤田泰太郎
  • Organizer: 日本農芸化学会2013年度大会
  • Place of Presentation: 東北大学, 仙台市
  • Year and Date: 2013-03-26
• [Presentation] 枯草菌 の炭素代謝と胞子形成開始の緊縮転写制御 (2013)
  • Author(s): 藤田泰太郎、東條繁郎、広岡和丈
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド、神戸市
• [Presentation] 枯草菌におけるラ ムノース異化に関わるyuxG-yulBCDEオペロンのYulBとCcpAによる制御機構 (2013)
  • Author(s): 広岡和丈、藤田泰太郎
  • Organizer: 2013年度グラム陽性菌ゲノム機能会議
  • Place of Presentation: 筑波山ホテル江戸屋、つくば市
• [Presentation] 枯草菌におけるラムノース異化に関わる遺伝子群の発現制御機構の解析 (2013)
  • Author(s): 広岡 和丈、藤田 泰太郎
  • Organizer: 日本農芸化学会2013年度大会
  • Place of Presentation: 東北大学（仙台市）
• [Presentation] 枯草菌Bacillus subtilis の胞子形成トリガーへの正の緊縮転写制御の関与 (2012)
  • Author(s): 東條繁郎, 広岡和丈, 藤田泰太郎
  • Organizer: 2012年度グラム陽性菌機能会議
  • Place of Presentation: 焼津グランドホテル, 焼津市
  • Year and Date: 2012-08-31
• [Presentation] 枯草菌の緊縮制御による胞子形成誘導機構の解明 (2012)
  • Author(s): 東條繁郎, 広岡和丈, 藤田泰太郎
  • Organizer: 2012年度日本農芸化学会大会
  • Place of Presentation: 京都女子大学, 京都市
  • Year and Date: 2012-03-24
• [Presentation] 枯草菌の緊縮制御による胞子形成誘導機構の解明 (2012)
  • Author(s): 東條繁郎、広岡和丈、藤田泰太郎
  • Organizer: 2012年度日本農芸化学会大会
  • Place of Presentation: 京都女子大学(京都市)
  • Year and Date: 2012-03-24
• [Presentation] 枯草菌緊縮制御の転写開始点の塩基種に依存した胞子形成誘導機構 (2012)
  • Author(s): 東條繁郎, 広岡和丈, 藤田泰太郎
  • Organizer: 第6回日本ゲノム微生物学会年会
  • Place of Presentation: 立教大学池袋キャンパス, 東京都
  • Year and Date: 2012-03-10
• [Presentation] 枯草菌緊縮制御の転写開始点の塩基種に依存した胞子形成誘導機構 (2012)
  • Author(s): 東條繁郎、広岡和丈、藤田泰太郎
  • Organizer: 第6回日本ゲノム微生物学会年会
  • Place of Presentation: 立教大学池袋キャンパス(東京都)
  • Year and Date: 2012-03-10
• [Presentation] 枯草菌Bacillus subtilisの胞子形成トリガーへの正の緊縮転写制御の関与 (2012)
  • Author(s): 藤田 泰太郎
  • Organizer: 2012年度グラム陽性菌機能会議
  • Place of Presentation: 焼津グランドホテル（焼津市）
• [Presentation] Construction of the metabolic networks and white biotechnology in the post-genome sequence era of Bacillus subtilis (2011)
  • Author(s): Yasutaro Fujita
  • Organizer: Sapporo Convention Center
  • Place of Presentation: Sapporo, Japan
  • Year and Date: 2011-09-10
  • Invited
• [Presentation] Heavy involvement of stringent transcription control dependeing on the adenine, or guanine species of the transcription initiation site in glucose and pyruvate metabolism in Bacillus subtilis (2011)
  • Author(s): Shigeo Tojo, Kazutake Hirooka, Yasutaro Fujita
  • Organizer: Sapporo Convention Center
  • Place of Presentation: Sapporo, Japan
  • Year and Date: 2011-09-10
• [Presentation] Construction of the metabolic networks and white biotechnology in the post-genome sequence era of Bacillus subtilis (2011)
  • Author(s): Yasutaro Fujita
  • Organizer: International Union of Microbiological Societies 2011 Congress (IUMS2011)
  • Place of Presentation: Sappro Convention Center(札幌市)(招待講演)
  • Year and Date: 2011-09-10
• [Presentation] Heavy involvement of stringent transcription control dependeing on the adenine, or guanine species of the transcription initiation site in glucose and pyruvate metabolism in Bacillus subtilis (2011)
  • Author(s): Shigeo Tojo, Kazutake Hirooka, Yasutaro Fujita
  • Organizer: International Union of Microbiological Societies 2011 Congress (IUMS2011)
  • Place of Presentation: Sapporo Business Inovation Center(札幌市)
  • Year and Date: 2011-09-10
• [Presentation] 枯草菌代謝ネットワークのカタボライト制御 (2011)
  • Author(s): 藤田泰太郎
  • Organizer: 2011年度グラム陽性菌機能会議
  • Place of Presentation: 福山大学社会連携研究推進センター,福山市
  • Year and Date: 2011-08-26
  • Invited
• [Presentation] 枯草菌代謝ネットワークのカタボライト制御 (2011)
  • Author(s): 藤田泰太郎
  • Organizer: 平成23年度グラム陽性菌ゲノム機能会議
  • Place of Presentation: 福山大学社会連携研究推進センター(福山市)(招待講演)
  • Year and Date: 2011-08-26
• [Presentation] 枯草菌の緊縮応答と胞子形成 (2011)
  • Author(s): 東條繁郎, 広岡和丈, 藤田泰太郎
  • Organizer: 2011年度グラム陽性菌ゲノム機能会議
  • Place of Presentation: 福山大学社会連携研究推進センター, 福山市
  • Year and Date: 2011-08-25
• [Presentation] 枯草菌の緊縮応答と胞子形成 (2011)
  • Author(s): 東條繁郎、広岡和丈、藤田泰太郎
  • Organizer: 平成23年度グラム陽性菌ゲノム機能会議
  • Place of Presentation: 福山大学社会連携研究推進センター(福山市)
  • Year and Date: 2011-08-25
• [Book] Creation of novel technologies for extracellular protein production toward the development of Bacillus subtilis genome factories pp. 3-15 in Microbial Production; From Genome Design to Cell Engineering (2014)
  • Author(s): Katsutoshi Ara, Kenji Manabe, Shenghao Liu, Yasushi Kageyama, Tadahiro Ozawa, Masatoshi Tohata, Keiji Endo, Kazuhisa Sawada, Nozomu Shibata, Akihito Kawahara, Kazuhiro Saito, Hiroshi Kodama, Yoshiharu Kimura, Katsuya Ozaki, Yoshinori Takema, Hiroshi Kakeshita, Kouji Nakamura, Kunio Yamane, Takeko Kodama, Junichi Sekiguchi, Takuya Morimoto, Ryosuke Kadoya, Shigehiko Kanaya, Yasutaro Fujita, Fujio Kawamura, Naotake Ogasawara
  • Total Pages: 306
  • Publisher: Springer Japan, Tokyo
• [Book] Microbial Production; From Genome Design to Cell Engineering (2014)
  • Author(s): Katsutoshi Ara, K. Manabe, S. Liu, Y. Kageyama, T. Ozawa, M. Tohata, K. Endo, K. Sawada, N. Shibata, A. Kawahara, K. Saito, H. Kodama, Y. Kimura, K. Ozaki, Y. Takema, H. Kakeshita, K. Nakamura, K. Yamane, T. Kodama, J. Sekiguchi, T. Morimoto, R. Kadoya, S. Kanaya, Y. Fujita, F. Kawamura, N. Ogasawara
  • Total Pages: 129
  • Publisher: Springer Japan, Tokyo
• [Book] Stringent transcription control of Escherichia coli and Bacillus subtilis (2012)
  • Author(s): Yasutaro Fujita, Shigeo Tojo, Kazutake Hirooka
  • Publisher: Research Signpost, Kerala, India
• [Book] Escherichia coli and Bacillus subtilis; the Frontiers of Molecular Microbiology Revisited (2012)
  • Author(s): Yasutaro Fujita
  • Total Pages: 362
  • Publisher: Research Signpost
• [Remarks] 藤田泰太郎-研究者-researchmap
  • URL: http://researchmap.jp/read0035996/
• [Remarks] 藤田 泰太郎ー研究者ーresearchmap
  • URL: http://researchmap.jp/read0035996/