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Elucidation of transcription network of stringent control in Bacillus subtilis and its application

Research Project

Project/Area Number 23380053
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied microbiology
Research InstitutionFukuyama University

Principal Investigator

FUJITA Yasutaro  福山大学, 生命工学部, 教授 (40115506)

Co-Investigator(Kenkyū-buntansha) HIROOKA Kazutake  福山大学, 生命工学部, 准教授 (20389068)
Project Period (FY) 2011-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥11,830,000 (Direct Cost: ¥9,100,000、Indirect Cost: ¥2,730,000)
Fiscal Year 2013: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2012: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2011: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Keywords緊縮応答 / 枯草菌 / 発現制御 / 応用微生物 / ゲノム / 胞子形成 / 緊縮転写制御 / プリン合成
Research Abstract

In Bacillus subtilis, stringent response evokes a GTP decrease and an ATP increase. As a result, positive and negative stringent transcription regulation of stringent operons possessing adenine and guanine in their transcription initiation sites, occur, respectively. Among positive stringent genes are kinA and kinB which are necessary for sporulation initiation. Operation of positive stringent transcription of kinA and kinB was found to be prerequisite for sporulation initiation.
As an approach to the applied field, guanine at the transcription initiation site of the pts operon under negative stringent control was converted to adenine, so that glucose metabolism was enhanced in the stringent conditions to result in more than 5-fold increase of isoleucine production by ilv-leu operon involved in branched-chain amino acid synthesis which is subjected to positive stringent transcription control.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Annual Research Report
  • 2011 Annual Research Report
  • Research Products

    (36 results)

All 2014 2013 2012 2011 Other

All Journal Article (7 results) (of which Peer Reviewed: 4 results) Presentation (23 results) (of which Invited: 3 results) Book (4 results) Remarks (2 results)

  • [Journal Article] Structural characterization of a ligand-bound form of Bacillus subtilis FadR involved in the regulation of fatty acid degradation2014

    • Author(s)
      Masahiro Fujihashi, Taiga Nakatani, Kazutake Hirooka, Hiroshi Matsuoka, Yasutaro Fujita, Kunio Miki
    • Journal Title

      Proteins

      Volume: (in press) Issue: 7 Pages: 1301-1310

    • DOI

      10.1002/prot.24496

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Journal Article] Expression of kinA and kinB of Bacillus] subtilis, necessary for sporulation initiation, is under positive stringent transcription control.2013

    • Author(s)
      Tojo,S.,Hirooka,K.,and Fujita,Y.
    • Journal Title

      Journal of Bacteriology

      Volume: 195 Issue: 8 Pages: 1656-1665

    • DOI

      10.1128/jb.02131-12

    • Related Report
      2013 Annual Research Report 2013 Final Research Report 2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Direct and indirect regulation of the ycnKJI operon involved in copper uptake through two transcriptional repressors, YcnK and CsoR, in Bacillus subtilis.2012

    • Author(s)
      Hirooka,K.,Edahiro,T.,Kimura,K.
    • Journal Title

      Journal of Bacteriology

      Volume: 194 Issue: 20 Pages: 5675-5687

    • DOI

      10.1128/jb.00919-12

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] 枯草菌Bacillus subtilis の胞子形成トリガーへの正の緊縮転写制御の関与2012

    • Author(s)
      東條繁郎, 広岡和丈, 藤田泰太郎
    • Journal Title

      福山大学生命工学部研究年報

      Volume: 11 Pages: 1-20

    • URL

      http://ci.nii.ac.jp/naid/110009465203

    • Related Report
      2013 Final Research Report
  • [Journal Article] 枯草菌Bacillus subtilisの胞子形成トリガーへの正の緊縮転写制御の 関与2012

    • Author(s)
      東條繁郎、藤田泰太郎
    • Journal Title

      福山大学生命工学部研究年報

      Volume: (11) Pages: 1-20

    • Related Report
      2012 Annual Research Report
  • [Journal Article] Identification of Aromatic Residues Critical to the DNA Binding and Ligand Response of the Bacillus subtilis QdoR (YxaF) Repressor Antagonized by Flavonoids2011

    • Author(s)
      Hirooka, K., and Fujita, Y.
    • Journal Title

      Bioscience, Biotechnology, and Biochemistry

      Volume: 75 Issue: 7 Pages: 1325-1334

    • DOI

      10.1271/bbb.110098

    • NAID

      10029329273

    • ISSN
      0916-8451, 1347-6947
    • Related Report
      2013 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Catabolite repression of the Bacillus subtilis FadR regulon, which is involved in fatty acid catabolism.2011

    • Author(s)
      Tojo, S., Satomura, T., Matsuoka, H., Hirooka, K., and Fujita Y.
    • Journal Title

      Journal of Bacteriology

      Volume: 193 Issue: 10 Pages: 2388-2395

    • DOI

      10.1128/jb.00016-11

    • Related Report
      2013 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Presentation] 枯草菌でのラムノース資化に関わるyuxG-yulBCDE オペロンにコードされる各タンパク質の機能解析2014

    • Author(s)
      広岡和丈, 藤田泰太郎
    • Organizer
      日本農芸化学会2014年度大会
    • Place of Presentation
      明治大学生田キャンパス, 東京都
    • Year and Date
      2014-03-28
    • Related Report
      2013 Final Research Report
  • [Presentation] 枯草菌でのラムノ ース資化に関わるyuxG-yulBCDEオペロンにコードされる各タンパク質の機能解析2014

    • Author(s)
      広岡和丈、藤田泰太郎
    • Organizer
      日本農芸化学会2014年度大会
    • Place of Presentation
      明治大学生田キャンパス、東京都
    • Related Report
      2013 Annual Research Report
  • [Presentation] 枯草菌の炭素代謝と胞子形成開始の緊縮転写制御2013

    • Author(s)
      藤田泰太郎, 東條繁郎, 広岡和丈
    • Organizer
      第36回日本分子生物学会年会
    • Place of Presentation
      神戸ポートアイランド, 神戸市
    • Year and Date
      2013-12-03
    • Related Report
      2013 Final Research Report
  • [Presentation] 枯草菌におけるラムノース異化に関わるyuxG-yulBCDE オペロンのYulB とCcpA による制御機構2013

    • Author(s)
      広岡和丈, 藤田泰太郎
    • Organizer
      2013年度グラム陽性菌ゲノム機能会議
    • Place of Presentation
      筑波山ホテル江戸屋, つくば市
    • Year and Date
      2013-09-07
    • Related Report
      2013 Final Research Report
  • [Presentation] Stringent transcription control of carbon metabolism and sporulation initiation in Bacillus subtilis2013

    • Author(s)
      Yasutaro Fujita, Shigeo Tojo, Kazutake Hirooka
    • Organizer
      7th International Conference on Gram-Positive Microorganisms
    • Place of Presentation
      Palazzo dei Congressi, Montecatini Terme, Italy
    • Year and Date
      2013-06-25
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Invited
  • [Presentation] 枯草菌におけるラムノース異化に関わる遺伝子群の発現制御機構の解析2013

    • Author(s)
      広岡和丈, 枝廣貴成, 木村晃輔, 藤田泰太郎
    • Organizer
      日本農芸化学会2013年度大会
    • Place of Presentation
      東北大学, 仙台市
    • Year and Date
      2013-03-26
    • Related Report
      2013 Final Research Report
  • [Presentation] 枯草菌 の炭素代謝と胞子形成開始の緊縮転写制御2013

    • Author(s)
      藤田泰太郎、東條繁郎、広岡和丈
    • Organizer
      第36回日本分子生物学会年会
    • Place of Presentation
      神戸ポートアイランド、神戸市
    • Related Report
      2013 Annual Research Report
  • [Presentation] 枯草菌におけるラ ムノース異化に関わるyuxG-yulBCDEオペロンのYulBとCcpAによる制御機構2013

    • Author(s)
      広岡和丈、藤田泰太郎
    • Organizer
      2013年度グラム陽性菌ゲノム機能会議
    • Place of Presentation
      筑波山ホテル江戸屋、つくば市
    • Related Report
      2013 Annual Research Report
  • [Presentation] 枯草菌におけるラムノース異化に関わる遺伝子群の発現制御機構の解析2013

    • Author(s)
      広岡 和丈、藤田 泰太郎
    • Organizer
      日本農芸化学会2013年度大会
    • Place of Presentation
      東北大学(仙台市)
    • Related Report
      2012 Annual Research Report
  • [Presentation] 枯草菌Bacillus subtilis の胞子形成トリガーへの正の緊縮転写制御の関与2012

    • Author(s)
      東條繁郎, 広岡和丈, 藤田泰太郎
    • Organizer
      2012年度グラム陽性菌機能会議
    • Place of Presentation
      焼津グランドホテル, 焼津市
    • Year and Date
      2012-08-31
    • Related Report
      2013 Final Research Report
  • [Presentation] 枯草菌の緊縮制御による胞子形成誘導機構の解明2012

    • Author(s)
      東條繁郎, 広岡和丈, 藤田泰太郎
    • Organizer
      2012年度日本農芸化学会大会
    • Place of Presentation
      京都女子大学, 京都市
    • Year and Date
      2012-03-24
    • Related Report
      2013 Final Research Report
  • [Presentation] 枯草菌の緊縮制御による胞子形成誘導機構の解明2012

    • Author(s)
      東條繁郎、広岡和丈、藤田泰太郎
    • Organizer
      2012年度日本農芸化学会大会
    • Place of Presentation
      京都女子大学(京都市)
    • Year and Date
      2012-03-24
    • Related Report
      2011 Annual Research Report
  • [Presentation] 枯草菌緊縮制御の転写開始点の塩基種に依存した胞子形成誘導機構2012

    • Author(s)
      東條繁郎, 広岡和丈, 藤田泰太郎
    • Organizer
      第6回日本ゲノム微生物学会年会
    • Place of Presentation
      立教大学池袋キャンパス, 東京都
    • Year and Date
      2012-03-10
    • Related Report
      2013 Final Research Report
  • [Presentation] 枯草菌緊縮制御の転写開始点の塩基種に依存した胞子形成誘導機構2012

    • Author(s)
      東條繁郎、広岡和丈、藤田泰太郎
    • Organizer
      第6回日本ゲノム微生物学会年会
    • Place of Presentation
      立教大学池袋キャンパス(東京都)
    • Year and Date
      2012-03-10
    • Related Report
      2011 Annual Research Report
  • [Presentation] 枯草菌Bacillus subtilisの胞子形成トリガーへの正の緊縮転写制御の関与2012

    • Author(s)
      藤田 泰太郎
    • Organizer
      2012年度グラム陽性菌機能会議
    • Place of Presentation
      焼津グランドホテル(焼津市)
    • Related Report
      2012 Annual Research Report
  • [Presentation] Construction of the metabolic networks and white biotechnology in the post-genome sequence era of Bacillus subtilis2011

    • Author(s)
      Yasutaro Fujita
    • Organizer
      Sapporo Convention Center
    • Place of Presentation
      Sapporo, Japan
    • Year and Date
      2011-09-10
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] Heavy involvement of stringent transcription control dependeing on the adenine, or guanine species of the transcription initiation site in glucose and pyruvate metabolism in Bacillus subtilis2011

    • Author(s)
      Shigeo Tojo, Kazutake Hirooka, Yasutaro Fujita
    • Organizer
      Sapporo Convention Center
    • Place of Presentation
      Sapporo, Japan
    • Year and Date
      2011-09-10
    • Related Report
      2013 Final Research Report
  • [Presentation] Construction of the metabolic networks and white biotechnology in the post-genome sequence era of Bacillus subtilis2011

    • Author(s)
      Yasutaro Fujita
    • Organizer
      International Union of Microbiological Societies 2011 Congress (IUMS2011)
    • Place of Presentation
      Sappro Convention Center(札幌市)(招待講演)
    • Year and Date
      2011-09-10
    • Related Report
      2011 Annual Research Report
  • [Presentation] Heavy involvement of stringent transcription control dependeing on the adenine, or guanine species of the transcription initiation site in glucose and pyruvate metabolism in Bacillus subtilis2011

    • Author(s)
      Shigeo Tojo, Kazutake Hirooka, Yasutaro Fujita
    • Organizer
      International Union of Microbiological Societies 2011 Congress (IUMS2011)
    • Place of Presentation
      Sapporo Business Inovation Center(札幌市)
    • Year and Date
      2011-09-10
    • Related Report
      2011 Annual Research Report
  • [Presentation] 枯草菌代謝ネットワークのカタボライト制御2011

    • Author(s)
      藤田泰太郎
    • Organizer
      2011年度グラム陽性菌機能会議
    • Place of Presentation
      福山大学社会連携研究推進センター,福山市
    • Year and Date
      2011-08-26
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] 枯草菌代謝ネットワークのカタボライト制御2011

    • Author(s)
      藤田泰太郎
    • Organizer
      平成23年度グラム陽性菌ゲノム機能会議
    • Place of Presentation
      福山大学社会連携研究推進センター(福山市)(招待講演)
    • Year and Date
      2011-08-26
    • Related Report
      2011 Annual Research Report
  • [Presentation] 枯草菌の緊縮応答と胞子形成2011

    • Author(s)
      東條繁郎, 広岡和丈, 藤田泰太郎
    • Organizer
      2011年度グラム陽性菌ゲノム機能会議
    • Place of Presentation
      福山大学社会連携研究推進センター, 福山市
    • Year and Date
      2011-08-25
    • Related Report
      2013 Final Research Report
  • [Presentation] 枯草菌の緊縮応答と胞子形成2011

    • Author(s)
      東條繁郎、広岡和丈、藤田泰太郎
    • Organizer
      平成23年度グラム陽性菌ゲノム機能会議
    • Place of Presentation
      福山大学社会連携研究推進センター(福山市)
    • Year and Date
      2011-08-25
    • Related Report
      2011 Annual Research Report
  • [Book] Creation of novel technologies for extracellular protein production toward the development of Bacillus subtilis genome factories pp. 3-15 in Microbial Production; From Genome Design to Cell Engineering2014

    • Author(s)
      Katsutoshi Ara, Kenji Manabe, Shenghao Liu, Yasushi Kageyama, Tadahiro Ozawa, Masatoshi Tohata, Keiji Endo, Kazuhisa Sawada, Nozomu Shibata, Akihito Kawahara, Kazuhiro Saito, Hiroshi Kodama, Yoshiharu Kimura, Katsuya Ozaki, Yoshinori Takema, Hiroshi Kakeshita, Kouji Nakamura, Kunio Yamane, Takeko Kodama, Junichi Sekiguchi, Takuya Morimoto, Ryosuke Kadoya, Shigehiko Kanaya, Yasutaro Fujita, Fujio Kawamura, Naotake Ogasawara
    • Total Pages
      306
    • Publisher
      Springer Japan, Tokyo
    • Related Report
      2013 Final Research Report
  • [Book] Microbial Production; From Genome Design to Cell Engineering2014

    • Author(s)
      Katsutoshi Ara, K. Manabe, S. Liu, Y. Kageyama, T. Ozawa, M. Tohata, K. Endo, K. Sawada, N. Shibata, A. Kawahara, K. Saito, H. Kodama, Y. Kimura, K. Ozaki, Y. Takema, H. Kakeshita, K. Nakamura, K. Yamane, T. Kodama, J. Sekiguchi, T. Morimoto, R. Kadoya, S. Kanaya, Y. Fujita, F. Kawamura, N. Ogasawara
    • Total Pages
      129
    • Publisher
      Springer Japan, Tokyo
    • Related Report
      2013 Annual Research Report
  • [Book] Stringent transcription control of Escherichia coli and Bacillus subtilis2012

    • Author(s)
      Yasutaro Fujita, Shigeo Tojo, Kazutake Hirooka
    • Publisher
      Research Signpost, Kerala, India
    • Related Report
      2013 Final Research Report
  • [Book] Escherichia coli and Bacillus subtilis; the Frontiers of Molecular Microbiology Revisited2012

    • Author(s)
      Yasutaro Fujita
    • Total Pages
      362
    • Publisher
      Research Signpost
    • Related Report
      2012 Annual Research Report
  • [Remarks] 藤田泰太郎-研究者-researchmap

    • URL

      http://researchmap.jp/read0035996/

    • Related Report
      2013 Final Research Report
  • [Remarks] 藤田 泰太郎ー研究者ーresearchmap

    • URL

      http://researchmap.jp/read0035996/

    • Related Report
      2013 Annual Research Report

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Published: 2011-04-06   Modified: 2019-07-29  

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