Novel function of ribosome involved in RsgA/RbfA
| Project/Area Number |
23380054
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied biochemistry
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| Research Institution | Hirosaki University |
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Principal Investigator |
HYOUTA Himeno 弘前大学, 農学生命科学部, 教授 (80208785)
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| Co-Investigator(Kenkyū-buntansha) |
MUTO Akira 弘前大学, 農学生命科学部, 研究員 (80034635)
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| Co-Investigator(Renkei-kenkyūsha) |
TAKEMOTO Chie 独立行政法人理化学研究所, 生命分子システム基盤研究領域, 上級研究員 (40306527)
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥18,850,000 (Direct Cost: ¥14,500,000、Indirect Cost: ¥4,350,000)
Fiscal Year 2013: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2012: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Fiscal Year 2011: ¥8,450,000 (Direct Cost: ¥6,500,000、Indirect Cost: ¥1,950,000)
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| Keywords | RsgA / YjeQ / RbfA / リボソーム / GTPase / 浸透圧ストレス / RimM |
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| Research Abstract |
From analyzing RsgA-deficient Escherichia coli strain and its revertant strains, we found a function of RsgA having a GTPase activity: RsgA dissociates RbfA from the small (30S) subunit during the last stage of ribosome maturation. In addition, the roles of three ribosome maturation factors including RsgA, RbfA and RimM in the ribosome maturation process have been investigated using biochemical and structural approaches.
We also found that RsgA deprivation provides resistance to osmotic (salt) stress with the cell. Resistance to osmotic (salt) stress is also provided by deprivation of other ribosome maturation factors.
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Report
(4 results)
Research Products
(45 results)
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[Journal Article] Dissecting the in vivo assembly of the 30S ribosomal subunit reveals the role of RimM and general features of the assembly process.2013
Author(s)
Guo, Q., Goto, S., Chen, Y., Feng, B., Xu, Y., Muto, A., Himeno, H., Deng, H., Lei, J., Gao, N.
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Journal Title
Nucleic Acids Res.
Volume: 41
Issue: 4
Pages: 2609-2620
DOI
Related Report
Peer Reviewed
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[Presentation] Recognition of mRNA length on the ribosome by tmRNA andSmpB.2013
Author(s)
Kurita, D., Miller, M.R., Muto, A., Buskirk, A.R., Himeno, H.
Organizer
Ribosomes Conference 2013
Place of Presentation
Napa Valley, California
Related Report
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[Presentation] Recognition of mRNA length on the ribosome by tmRNA and SmpB.2013
Author(s)
Kurita, D., Miller, M.R., Muto, A., Buskirk, A.R., Himeno, H.
Organizer
Ribosomes Conference 2013
Place of Presentation
Napa Valley, California
Related Report
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[Presentation] The first translocation in trans-translation.2012
Author(s)
Takada, K., Kurita, D., Naoe, C., Yokogawa, T., Himeno, H., Takemoto, C.
Organizer
第14回日本RNA学会年会
Place of Presentation
仙台
Related Report
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[Presentation]2011
Author(s)
後藤史門,武藤あきら,姫野俵太
Organizer
第34回日本分子生物学会
Place of Presentation
横浜
Related Report
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