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High-Throughput Genetic Identification of Regulatory Factors Involved in the Regulation of Seed Oil Storage Using Bioluminescence Automatic Monitoring System

Research Project

Project/Area Number 23380057
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied biochemistry
Research InstitutionChubu University

Principal Investigator

NAKAMURA Kenzo  中部大学, 応用生物学部, 教授 (80164292)

Co-Investigator(Kenkyū-buntansha) ISHIURA Masahiro  名古屋大学, 遺伝子実験施設, 教授 (20132730)
Project Period (FY) 2011-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥18,980,000 (Direct Cost: ¥14,600,000、Indirect Cost: ¥4,380,000)
Fiscal Year 2013: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2012: ¥7,280,000 (Direct Cost: ¥5,600,000、Indirect Cost: ¥1,680,000)
Fiscal Year 2011: ¥8,970,000 (Direct Cost: ¥6,900,000、Indirect Cost: ¥2,070,000)
Keywords種子成熟 / 種子発芽 / 油脂貯蔵 / 突然変異株 / 発光レポーター / 次世代シーケンサー / 植物種子 / 油脂 / 遺伝子発現抑制 / ルシフェラーゼ発光 / オレオシン / 突然変異体 / 次世代シーケンサ
Outline of Final Research Achievements

We prepared Arabidopsis plants that express LUC reporter gene under the control of promoters from genes important in the biosynthesis and storage of seed oil. By using automatic bioluminescence monitoring system, we screened several hundred thousands of plants for mutants with altered expression patterns of LUC reporter gene. In addition to AOTA1, a novel factor involved in the activation of DGAT1 gene for the final step of seed oil synthesis, we could identify several additional candidate genes potentially involved in the activation of DGAT1. Furthermore, we could identify a novel gene DROL1 coding for a factor potentially involved in the repression of OleS3 gene for seed oil storage after seed germination.

Report

(4 results)
  • 2014 Final Research Report ( PDF )
  • 2013 Annual Research Report
  • 2012 Annual Research Report
  • 2011 Annual Research Report
  • Research Products

    (22 results)

All 2015 2014 2013 2012 2011

All Journal Article (8 results) (of which Peer Reviewed: 8 results,  Open Access: 1 results) Presentation (14 results) (of which Invited: 1 results)

  • [Journal Article] Wound-induced expression of DEFECTIVE IN ANTHER DEHISCENCEI and DAD1-like lipase genes is mediated by both C ORONATINE INSENSITIVE1-dependent and independent pathways in Arabidopsis thaliana.2014

    • Author(s)
      Ruduś, I. ら12名
    • Journal Title

      Plant Cell Rep.

      Volume: 33(印刷中) Issue: 6 Pages: 849-860

    • DOI

      10.1007/s00299-013-1561-8

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A duplicated NUCLEOLIN gene in Arabidopsis (AtNUC2) is required for rDNA methylation and organization of silent 45S rRNA genes.2014

    • Author(s)
      Durut, N., Abou-Ellail, M., Pontvianne, F., Comella, P., Chandra Das., S, Kojima, H., Ukai, S., DeBures, A., Echeverria, M., Bouvet, P., Nakamura, K. and Saez-Vasquez, J.
    • Journal Title

      Plant Cell

      Volume: 26 Issue: 3 Pages: 1330-1344

    • DOI

      10.1105/tpc.114.123893

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Development and disintegration of tapetum-specific lipid-accumulating organelles, elaioplasts and tapetosomes, in Arabidopsis thaliana and Brassica napus2013

    • Author(s)
      Suzuki, Toshiya
    • Journal Title

      Plant Science

      Volume: 207 Pages: 25-36

    • DOI

      10.1016/j.plantsci.2013.02.008

    • Related Report
      2013 Annual Research Report 2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Transgenic sweet potato expressing thionin from barley gives resistance to black rot disease caused by Ceratosystis fimbriata in leaves and storage roots.2012

    • Author(s)
      Muramoto, N., ら10名
    • Journal Title

      Plant Gell Rep.

      Volume: 31 Issue: 6 Pages: 987-997

    • DOI

      10.1007/s00299-011-1217-5

    • Related Report
      2012 Annual Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Phase-dependent generation and transmission of time information by the KaiABC circadian clock oscillator through SasA-KaiC interaction in cyanobacteria.2012

    • Author(s)
      Valencia S, J, et al
    • Journal Title

      Genes to Cells

      Volume: 17 Issue: 5 Pages: 398-419

    • DOI

      10.1111/j.1365-2443.2012.01597.x

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The roles of the dimeric and tetrameric structures of the clock protein KaiB in the generation of circadian oscillations in cyanobacteria2012

    • Author(s)
      Murakami R, Mutoh R, Iwase R, Furukawa Y, Imada K, Onai K, Morishita M, Yasui S, Ishii K, Valencia Swain JO, Uzumaki T, Namba K, Ishiura M.
    • Journal Title

      J Biol Chem.

      Volume: 287 Issue: 35 Pages: 29506-29515

    • DOI

      10.1074/jbc.m112.349092

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Aquaporin AqpZ is involved in cell volume regulation and sensitivity to osmotic stress in Synechocystis sp. strain PCC 6803.2012

    • Author(s)
      Akai, M.
    • Journal Title

      J Bacteriol

      Volume: 194 Issue: 24 Pages: 6828-6836

    • DOI

      10.1128/jb.01665-12

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Mutations in MYB3R1 and MYB3R4 cause pleiotropic developmental defects and preferential down-regulation of multiple G2/M-specific genes in Arabidopsis thaliana.2011

    • Author(s)
      Haga, N., ら11名
    • Journal Title

      Plant Physiol.

      Volume: 157 Issue: 2 Pages: 706-717

    • DOI

      10.1104/pp.111.180836

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Presentation] シロイヌナズナにおける種子成熟遺伝子の発芽後の発現抑制に関わる新規因子の解析2015

    • Author(s)
      河合都妙、鈴木孝征、小内清、前尾健一郎、石浦正寛、東山哲也、中村研三
    • Organizer
      第56回日本植物生理学会年会
    • Place of Presentation
      東京農業大学世田谷キャンパス
    • Year and Date
      2015-03-16 – 2015-03-18
    • Related Report
      2013 Annual Research Report
  • [Presentation] シロイヌナズナ種子油脂合成遺伝子AtDGAT1の発現活性化に関わる因子の解析2014

    • Author(s)
      河合都妙、伊藤節嗣、橋本実佳、前尾健一郎、中村研三
    • Organizer
      第27回植物脂質シンポジウム
    • Place of Presentation
      静岡市産学交流センター
    • Year and Date
      2014-11-28 – 2014-11-29
    • Related Report
      2013 Annual Research Report
  • [Presentation] 種子成熟遺伝子の発芽後の発現抑制を制御する因子の網羅的スクリーニング2014

    • Author(s)
      河合都妙、鈴木孝征、小内清、前尾健一郎、石浦正寛、東山哲也、中村研三
    • Organizer
      第37回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2014-11-25 – 2014-11-27
    • Related Report
      2013 Annual Research Report
  • [Presentation] 発光レポーターを使った種子成熟遺伝子の発芽後抑制に異常を示すシロイヌナズナ変異株の 網羅的スクリーニング2014

    • Author(s)
      河合都妙、小内清、前尾健一郎、石浦正寛、中村研三
    • Organizer
      第55回日本植物生理学会年会
    • Place of Presentation
      富山大学五福キャンパス
    • Year and Date
      2014-03-18 – 2014-03-20
    • Related Report
      2013 Annual Research Report
  • [Presentation] 種子成熟遺伝子の発現におけるB3 転写因子HSI2とHSL1の機能2014

    • Author(s)
      河合都妙、鵜飼聖子、御堂育子、塚越啓央、石浦正寛、中村研三
    • Organizer
      第55回日本植物生理学会年会
    • Place of Presentation
      富山大学五福キャンパス
    • Year and Date
      2014-03-18 – 2014-03-20
    • Related Report
      2013 Annual Research Report
  • [Presentation] 高速シーケンサーを用いたシロイヌナズナ変異株の原因遺伝子同定システム Mitsucal の開発2013

    • Author(s)
      鈴木孝征ら9名
    • Organizer
      第54回日本植物生理学会年会
    • Place of Presentation
      岡山大学
    • Related Report
      2012 Annual Research Report
  • [Presentation] シロイヌナズナ種子油脂合成遺伝子の発現活性化に係わるDREBサブファミリー転写因子2013

    • Author(s)
      伊藤節嗣ら5名
    • Organizer
      第54回日本植物生理学会年会
    • Place of Presentation
      岡山大学
    • Related Report
      2012 Annual Research Report
  • [Presentation] 発光レポーターを使用した種子成熟プログラム抑制機構の解析2013

    • Author(s)
      河合都妙ら計8名
    • Organizer
      第54回日本植物生理学会年会
    • Place of Presentation
      岡山大学
    • Related Report
      2012 Annual Research Report
  • [Presentation] シロイヌナズナ種子油脂合成系遺伝子の新奇活性化因子の遺伝学的同定2012

    • Author(s)
      橋本実佳
    • Organizer
      第53回日本植物生理学会年会
    • Place of Presentation
      京都産業大学(京都市)
    • Year and Date
      2012-03-18
    • Related Report
      2011 Annual Research Report
  • [Presentation] シロイヌナズナB3因子HSI2、HSL1による種子成熟プログラムの抑制機構2012

    • Author(s)
      鵜飼聖子
    • Organizer
      第53回日本植物生理学会年会
    • Place of Presentation
      京都産業大学(京都市)
    • Year and Date
      2012-03-16
    • Related Report
      2011 Annual Research Report
  • [Presentation] WRI1 サブファミリーデュアルAP2 ドメイン転写因子の脂肪酸合成制御における役割分担2012

    • Author(s)
      浅野翔一ら計7名
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      福岡国際会議場
    • Related Report
      2012 Annual Research Report
  • [Presentation] Transcriptional control of seed oil accumulation in Arabidopsis2012

    • Author(s)
      Nakamura, K.
    • Organizer
      International Symposiumu on Recent Advances in Plant Biotechnology
    • Place of Presentation
      Ton Duc Thang University, Ho Chi Minh City, Viet Nam
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] Large scale screenings of Arabidopsis seed oil mutant by using bioluminescence monitoring system2011

    • Author(s)
      Kawai, T.
    • Organizer
      The 4th Asian Symposium on Plant Lipids
    • Place of Presentation
      Univ.Hong Kong (Hong Kong)
    • Year and Date
      2011-12-03
    • Related Report
      2011 Annual Research Report
  • [Presentation] Repression of genes involed in seed oil accumulation after seed germination2011

    • Author(s)
      Nakamura, K.
    • Organizer
      The 4th Asian Symposium on Plant Lipids
    • Place of Presentation
      Univ.Hong Kong (Honh Kong)
    • Year and Date
      2011-12-03
    • Related Report
      2011 Annual Research Report

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Published: 2011-04-06   Modified: 2019-07-29  

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