Molecular genetic studies on flowering control by VOZ1 and VOZ2 in Arabidopsis

• Project/Area Number: 23380058
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied biochemistry
• Research Institution: Kyoto University
• Principal Investigator: KOHCHI Takayuki 京都大学, 生命科学研究科, 教授 (40202056)
• Co-Investigator(Renkei-kenkyūsha): ISHIZAKI Kimitsune 神戸大学, 理学研究科, 准教授 (00452293)
• Project Period (FY): 2011-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥18,850,000 (Direct Cost: ¥14,500,000、Indirect Cost: ¥4,350,000); Fiscal Year 2013: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2012: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2011: ¥10,790,000 (Direct Cost: ¥8,300,000、Indirect Cost: ¥2,490,000)
• Keywords: シロイヌナズナ / フィトクロム / 転写制御 / 花成制御 / 植物生理学 / 光受容 / 環境応答 / 低温応答 / NAC転写因子 / 光センシング

Research Abstract

The phase transition of vegetative to reproductive growth in plants is regulated by various environmental factors including day length and light quality. Two Arabidopsis thaliana genes, VASCULAR PLANT ONE ZINC FINGER1 and VOZ2 mediated phyB-dependent flowering regulation. By mutant complementation assays, VOZ1 and VOZ2 expressed in vascular bundles functioned as transcriptional regulators in nucleus. Genetic and molecular researches suggested that VOZ1 and VOZ2 promote growth-phase transition to flowering by down-regulating FLC and its closely related homologs MAF1 to MAF5 by a pathway that is independent to vernalization pathway.

Research Products

• [Journal Article] Evolutionary insights into photoregulation of the cell cycle in the green lineage (2013)
  • Author(s): Nishihama, R and Kohchi, T
  • Journal Title: Curr. Opinion Plant Biol Volume: 16 Issue: 5 Pages: 630-637
  • DOI: 10.1016/j.pbi.2013.07.006
  • Peer Reviewed
• [Journal Article] The phytochrome-interacting VASCULAR PLANT ONE-ZINC FINGERl and VOZ2 redundantly regulate flowering in Arabidopsis (2012)
  • Author(s): 安居佑季子, 向川佳子, 上本允大, 横藤陽, 硯亮太, 西谷亜依子, 河内孝之
  • Journal Title: The Plant Cell Volume: 24 Issue: 8 Pages: 3248-3263
  • DOI: 10.1105/tpc.112.101915
  • Peer Reviewed
• [Journal Article] Visualization of auxin-mediated transcriptional activation using a common auxin-responsive reporter system in the liverwort Marchantia polymorpha (2012)
  • Author(s): Ishizaki K., Nonomura M., Kato H., Yamato K.T., Kohchi T.
  • Journal Title: Journal of Plant Research Volume: 125 Issue: 5 Pages: 826-834
  • DOI: 10.1007/s10265-012-0477-7
  • Peer Reviewed
• [Journal Article] VASCULAR PLANT ONE-ZINC FINGER1 and VOZ2 repress the FLOWERING LOCUS C clade members to control flowering time in Arabidopsis
  • Author(s): Yasui, Y. and Kohchi, T
  • Journal Title: Biosci. Biotechnol. Biochem Volume: (in press)
  • NAID: 120005676666
• [Presentation] シロイヌナズナにおけるフィトクロム相互作用因子VOZ を介した凍結体制の制御機構 (2014)
  • Author(s): 横藤陽, 安居佑季子, 河内孝之
  • Organizer: 第55回日本植物生理学会年会
  • Place of Presentation: 富山大学
  • Year and Date: 2014-03-20
• [Presentation] フィトクロム相互作用因子VASCULAR PLANT ONE-ZINC FINGER (VOZ)による暗所における植物の生長制御の解析 (2014)
  • Author(s): 安居佑季子, 横藤陽, 平野博之, 河内孝之
  • Organizer: 第55回日本植物生理学会年会
  • Place of Presentation: 富山大学
  • Year and Date: 2014-03-18
• [Presentation] フィトクロム相互作用因子VASCULAR PLANT ONE-ZINC FINGER (VOZ)による暗所における植物の生長制御の解析 (2014)
  • Author(s): 安居佑季子、横藤陽、平野博之、河内孝之
  • Organizer: 第55回 日本植物生理学会年会
  • Place of Presentation: 富山大学（富山市）
• [Presentation] シロイヌナズナにおけるフィトクロム相互作用因子VOZを介した凍結体制の制御機構 (2014)
  • Author(s): 横藤陽、安居佑季子、河内孝之
  • Organizer: 第55回 日本植物生理学会年会
  • Place of Presentation: 富山大学（富山市）
• [Presentation] 花成促進に機能するフィトクロム相互作用因子VASCULAR PLANT ONE ZINC FINGER (VOZ)の光質に依存したシグナル伝達の解析 (2013)
  • Author(s): 安居佑季子, 上本允大, 横藤陽, 河内孝之
  • Organizer: 第54回日本植物生理学会年会
  • Place of Presentation: 岡山大学
  • Year and Date: 2013-03-23
• [Presentation] 花成を促進するphyB相互作用因子VOZ は光質により制御される (2012)
  • Author(s): 安居佑季子, 上本允大, 河内孝之
  • Organizer: 第17回日本光生物学協会年会
  • Place of Presentation: 大阪大学
  • Year and Date: 2012-08-16
• [Presentation] Functional analysis of phytochrome-interacting factor VOZ in flowering pathway (2012)
  • Author(s): Yukiko Yasui, Mitsuhiro Uemoto, Keiko Mukougawa, Yusuke Nakai, Yoichi Nakahira, Masahik.H. Sato, Takayuki. Kohchi
  • Organizer: The 5th Asia and Oceania Conference for Photobiology
  • Place of Presentation: 奈良県新公会堂
  • Year and Date: 2012-07-31
• [Presentation] Light regulated phytochrome-interacting proteins VOZ1 and VOZ2 promote flowering in Arabidopsis (2012)
  • Author(s): Yukiko Yasui, Mitsuhiro Uemoto, and Takayuki Kohchi
  • Organizer: The 1st International Symposium on Plant Environmental Sensing
  • Place of Presentation: 東大寺文化センター
  • Year and Date: 2012-03-19
• [Presentation] Light regulated phytochrome-interacting proteins VOZ1 and VOZ2 promote flowering in Arabidopsis (2012)
  • Author(s): Y.Yasui, M.Uemoto, T.Kohchi
  • Organizer: The 1st International Symposium on Plant Environmental Sensing
  • Place of Presentation: 東大寺文化センター(奈良県)
  • Year and Date: 2012-03-19
• [Presentation] 花成経路で機能するフィトクロム相互作用因子VOZのシグナル伝達解析 (2012)
  • Author(s): 安居佑季子, 上本允大, 佐藤雅彦, 河内孝之
  • Organizer: 第53回日本植物生理学会年会
  • Place of Presentation: 京都産業大学
  • Year and Date: 2012-03-17
• [Presentation] The light regulated phytochrome- interacting VASCULAR PLANT ONE-ZINC FINGER1 and VOZ2 redundantly promote flowering in Arabidopsis (2012)
  • Author(s): Yukiko Yasui, Mitsuhiro Uemoto, Akira Yokofuji, and Takayuki Kohchi
  • Organizer: The 10th International Congress on Plant Molecular Biology
  • Place of Presentation: 済州国際会議場,韓国, 済州島
• [Presentation] The light regulated phytochrome-interacting VASCULAR PLANT ONE-ZINC FINGER1 and VOZ2 redundantly promote flowering in Arabidopsis (2012)
  • Author(s): Yukiko Yasui, Mitsuhiro Uemoto, Akira Yokofuji, and Takayuki Kohchi
  • Organizer: The 10th International Congress on Plant Molecular Biology
  • Place of Presentation: 済州国際会議場（韓国、済州島）
• [Presentation] Functional analysis of phytochrome-interacting factor VOZ in flowering pathway (2011)
  • Author(s): Y.Yasui, M.Uemoto, K.Mukougawa, Y.Nakai, Y.Nakahira, M.H.Sato, T.Kohchi
  • Organizer: 5th Asia and Oceania Conference for Photobiology
  • Place of Presentation: 奈良県新公会堂(奈良県)
  • Year and Date: 2011-07-31
• [Presentation] 花成促進に機能するフィトクロム相互作用因子VASCULAR PLANT ONE ZINC FINGER (VOZ)の光質に依存したシグナル伝達の解析
  • Author(s): 安居佑季子、上本允大、横藤陽、河内 孝之
  • Organizer: 第54回日本植物生理学会年会
  • Place of Presentation: 岡山大学（岡山市）
• [Presentation] 花成を促進するphyB相互作用因子VOZは光質により制御される
  • Author(s): 安居 佑季子, 上本 允大, 河内 孝之
  • Organizer: 第17回 日本光生物学協会年会
  • Place of Presentation: 大坂大学（吹田市）
• [Remarks]
  • URL: http://www.lif.kyoto-u.ac.jp/labs/plantmb/