| Budget Amount *help |
¥14,300,000 (Direct Cost: ¥11,000,000、Indirect Cost: ¥3,300,000)
Fiscal Year 2013: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2012: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2011: ¥8,580,000 (Direct Cost: ¥6,600,000、Indirect Cost: ¥1,980,000)
|
|---|
| Research Abstract |
Allosamidin, a chitinase inhibitor, shows anti-asthmatic activity toward model mice. Chitinase (AMCase) and chitinase-like proteins (BRP39, Ym1, and Ym2) are possible candidates as target molecules for anti-asthmatic activity of allosamidin. To Recombinant protein of each of the four proteins was prepared and their binding activity toward allosamidin probes, which were newly prepared, was investigated. All the recombinant proteins bound to allosamidin with low Kd values around 10-100 nM. It was found that BRP39 shows protecting activity against death of mouse primary cells. Allosamidin did not inhibit the activity of BRP39 for the cells.
|
