Study on the function of ND1 subunit, a key subunit of respiratory complex I
| Project/Area Number |
23380064
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Bioproduction chemistry/Bioorganic chemistry
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| Research Institution | Kyoto University |
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Principal Investigator |
MIYOSHI Hideto 京都大学, (連合)農学研究科(研究院), 教授 (20190829)
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| Co-Investigator(Renkei-kenkyūsha) |
MURAI Masatoshi 京都大学, 大学院・農学研究科, 助教 (80543925)
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥18,460,000 (Direct Cost: ¥14,200,000、Indirect Cost: ¥4,260,000)
Fiscal Year 2013: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2012: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2011: ¥8,320,000 (Direct Cost: ¥6,400,000、Indirect Cost: ¥1,920,000)
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| Keywords | 細胞機能調節物質 / ミトコンドリア呼吸鎖酵素 / 複合体-I / アセトゲニン / 光親和性標識 / トシルケミストリー / ミトコンドリア / 阻害剤 / 呼吸鎖複合体-I / ND1サブユニット / 呼吸鎖阻害剤 |
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| Research Abstract |
To elucidate the functional and structural aspects of the ND1 subunit, which is a key subunit of the membrane domain of bovine heart mitochondrial complex I (NADH-quinone oxidoreductase), I synthesized unique inhibitor probes acting to the ND1 and identified their binding sites via photoaffinity labeling experiment. In addition, I succeeded in site-specific chemical labeling (alkynylation) via ligand-directed tosyl (LDT) chemistry using acetogenin as a high affinity ligand against complex I.
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Report
(4 results)
Research Products
(22 results)
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[Journal Article] Critical role of a methyl group on the γ-lactone ring of annonaceous acetogenins in the potent inhibition of mitochondrial complex I2013
Author(s)
Kojima, N., Abe, M., Suga, Y., Ohtsuki, K., Tanaka, T., Iwasaki, H., Yamashita, M., and Miyoshi, H
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Journal Title
Bioorg. Med. Chem. Lett
Volume: 23
Pages: 1217-1219
Related Report
Peer Reviewed
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[Journal Article] Synthesis and characterization of photoaffinity probe of acetogenin, a strong inhibitor of mitochondrial complex I2011
Author(s)
Yamamoto, S., Abe, M., Nakanishi, S., Murai, M., and Miyoshi, H
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Journal Title
Tetrahedron Lett
Volume: 52
Pages: 3090-3093
Related Report
Peer Reviewed
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