| Budget Amount *help |
¥18,460,000 (Direct Cost: ¥14,200,000、Indirect Cost: ¥4,260,000)
Fiscal Year 2013: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2012: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2011: ¥8,320,000 (Direct Cost: ¥6,400,000、Indirect Cost: ¥1,920,000)
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| Research Abstract |
To elucidate the functional and structural aspects of the ND1 subunit, which is a key subunit of the membrane domain of bovine heart mitochondrial complex I (NADH-quinone oxidoreductase), I synthesized unique inhibitor probes acting to the ND1 and identified their binding sites via photoaffinity labeling experiment. In addition, I succeeded in site-specific chemical labeling (alkynylation) via ligand-directed tosyl (LDT) chemistry using acetogenin as a high affinity ligand against complex I.
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