Studies on the regulation of brassinosteroid biosynthesis

• Project/Area Number: 23380066
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Bioorganic chemistry
• Research Institution: The Institute of Physical and Chemical Research
• Principal Investigator: FUJIOKA Shozo 独立行政法人理化学研究所, 長田抗生物質研究室, 副主任研究員 (60165355)
• Project Period (FY): 2011-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000); Fiscal Year 2014: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2013: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2012: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2011: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
• Keywords: ブラシノステロイド / 生合成 / 代謝 / シロイヌナズナ / イネ / 阻害剤 / シグナル伝達 / 生合成調節 / ユビキチンリガーゼ / 不活性化 / アシル基転移酵素 / トウモロコシ / 性決定

Outline of Final Research Achievements

This study established novel pathways of brassinosteroid (BR) biosynthesis as well as main paths leading to biologically active BRs. Analyses of BR dwarf mutants revealed that some acyltransferases play important roles in regulating BR levels. Inhibitor of BIN2, a negative regulator in BR signaling, was found to reduce endogenous BR levels.

Research Products

• [Journal Article] Arabidopsis gulliver1/superroot2-7 identifies a metabolic basis for auxin and brassinosteroid synergy (2014)
  • Author(s): Puna M. Maharjan, Brian P. Dilkes, Shozo Fujioka, Ales Pencik, Karin Ljung, Meike Burow, Barbara A. Halkier, and Sunghwa Choe
  • Journal Title: Plant Journal Volume: 80 Issue: 5 Pages: 797-808
  • DOI: 10.1111/tpj.12678
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Darkness and gulliver2/phyB mutation decrease the abundance of phosphorylated BZR1 to activate brassinosteroid signaling in Arabidopsis (2014)
  • Author(s): Bokyung Kim, Yu Jeong Jeong, Claudia Corvalan, Shozo Fujioka, Seoae Cho, Taesung Park, and Sunghwa Choe
  • Journal Title: Plant Journal Volume: 77 Issue: 5 Pages: 737-747
  • DOI: 10.1111/tpj.12423
  • Peer Reviewed
• [Journal Article] Arabidopsis brassinosteroid-overproducing gulliver3-D/dwarf4-D mutants exhibit altered responses to jasmonic acid and pathogen (2013)
  • Author(s): Bokyung Kim, Shozo Fujioka, Mi Kwon, Jihyung Jeon, and Sunghwa Choe
  • Journal Title: Plant Cell Reports Volume: 32 Issue: 7 Pages: 1139-1149
  • DOI: 10.1007/s00299-012-1381-2
  • Peer Reviewed
• [Journal Article] Auxins increase expression of the brassinosteroid receptor and brassinosteroid-responsive genes in Arabidopsis (2013)
  • Author(s): Tomoaki Sakamoto and Shozo Fujioka
  • Journal Title: Plant Signaling & Behavior Volume: 8 Issue: 4 Pages: e23509-e23509
  • DOI: 10.4161/psb.23509
  • Peer Reviewed
• [Journal Article] An E3 ubiquitin ligase, ERECT LEAF1, functions in brassinosteroid signaling of rice (2013)
  • Author(s): Tomoaki Sakamoto, Hidemi Kitano, and Shozo Fujioka
  • Journal Title: Plant Signaling & Behavior Volume: 8 Issue: 11 Pages: e27117-e27117
  • DOI: 10.4161/psb.27117
  • Peer Reviewed
• [Journal Article] Brassinosteroids regulate plant growth through distinct signaling pathways in Selaginella and Arabidopsis (2013)
  • Author(s): Jinyeong Cheon, Shozo Fujioka, Brian P. Dilkes, and Sunghwa Choe
  • Journal Title: PLoS ONE Volume: 8
  • Peer Reviewed
• [Journal Article] Genetic background influences brassinosteroid-related mutant phenotypes in rice (2013)
  • Author(s): Tomoaki Sakamoto
  • Journal Title: American Journal of Plant Sciences Volume: 4 Issue: 02 Pages: 212-221
  • DOI: 10.4236/ajps.2013.42028
  • Peer Reviewed
• [Journal Article] Auxin signal transcription factor regulates expression of the brassinosteroid receptor gene in rice (2013)
  • Author(s): Tomoaki Sakamoto
  • Journal Title: Plant Journal Volume: 73 Issue: 4 Pages: 676-688
  • DOI: 10.1111/tpj.12071
  • Peer Reviewed
• [Journal Article] Homeostasis of brassinosteroids regulated by DRL1, a putative acyltransferase in Arabidopsis (2013)
  • Author(s): Wenjiao Zhu
  • Journal Title: Molecular Plant Volume: 6 Issue: 2 Pages: 546-558
  • DOI: 10.1093/mp/sss144
  • Peer Reviewed
• [Journal Article] Genetic variation in plant CYP51s confers resistance against voriconazole, a novel inhibitor of brassinosteroid-dependent sterol biosynthesis (2013)
  • Author(s): Wilfried Rozhon
  • Journal Title: PLoS ONE Volume: 8
  • Peer Reviewed
• [Journal Article] Genetic evidence for the reduction of brassinosteroid levels by a BAHD acyltransferase-like protein in Arabidopsis (2012)
  • Author(s): Hyungmin Roh
  • Journal Title: Plant Physiology Volume: 159 Issue: 2 Pages: 696-709
  • DOI: 10.1104/pp.112.197202
  • Peer Reviewed
• [Journal Article] Overexpression of 3b-hydroxysteroid dehydrogenases/C-4 decarboxylases causes growth defects possibly due to abnormal auxin transport in Arabidopsis (2012)
  • Author(s): Bokyung Kim
  • Journal Title: Molecules and Cells Volume: 34 Issue: 1 Pages: 77-84
  • DOI: 10.1007/s10059-012-0102-6
  • Peer Reviewed
• [Journal Article] Rice CYP90D2 and CYP90D3 catalyze C-23 hydroxylation of brassinosteroids in vitro (2012)
  • Author(s): Tomoaki Sakamoto
  • Journal Title: Plant Physiology and Biochemistry Volume: 58 Pages: 220-226
  • Peer Reviewed
• [Journal Article] CYP901A1/CPD, a brassinosteroid biosynthetic cytochrome P450 of Arabidopsis, catalyzes C-3 oxidation (2012)
  • Author(s): Toshiyuki Ohnishi
  • Journal Title: Journal of Biological Chemistry Volume: 287 Issue: 37 Pages: 31551-31560
  • DOI: 10.1074/jbc.m112.392720
  • Peer Reviewed
• [Journal Article] Constitutive activation of brassinosteroid signaling in the Arabidopsis elongated-D/bak1 mutant (2012)
  • Author(s): Yuhee Chung
  • Journal Title: Plant Molecular Biology Volume: 80 Issue: 4-5 Pages: 489-501
  • DOI: 10.1007/s11103-012-9963-5
  • Peer Reviewed
• [Journal Article] New alleles of rice ebisu dwarf (d2) mutant show both brassinosteroid-deficient and -insensitive phenotypes (2012)
  • Author(s): Tomoaki Sakamoto
  • Journal Title: American Journal of Plant Sciences Volume: 3 Issue: 12 Pages: 1699-1707
  • DOI: 10.4236/ajps.2012.312208
  • Peer Reviewed
• [Journal Article] Synthesis of hydroxylated sterols in transgenic Arabidopsis plants alters growth and steroid metabolism (2011)
  • Author(s): Lisa Beste
  • Journal Title: Plant Physiology Volume: 157 Issue: 1 Pages: 426-440
  • DOI: 10.1104/pp.110.171199
  • Peer Reviewed
• [Journal Article] Brassinosteroid control of sex determination in maize (2011)
  • Author(s): Thomas Hartwig
  • Journal Title: Proc.Natl.Acad.Sci.USA Volume: 108 Issue: 49 Pages: 19814-19819
  • DOI: 10.1073/pnas.1108359108
  • Peer Reviewed
• [Presentation] 葉面積拡大を促進する新規ブラシノステロイド情報伝達因子BIL8の細胞内機能 (2015)
  • Author(s): 山上あゆみ、中田元基、市川尚斉、松井南、藤岡昭三、長田裕之、久城哲夫、浅見忠男、中野雄司、
  • Organizer: 第56回日本植物生理学会年会
  • Place of Presentation: 東京都・東京農業大学
  • Year and Date: 2015-03-17
• [Presentation] 花茎伸長を促進するブラシノステロイド情報伝達因子BIL7による転写因子BIL1/BZR1の活性化機構 (2015)
  • Author(s): 宮地朋子、市川尚斉、松井南、藤岡昭三、長田裕之、浅見忠男、中野雄司
  • Organizer: 第56回日本植物生理学会年会
  • Place of Presentation: 東京都・東京農業大学
  • Year and Date: 2015-03-17
• [Presentation] 細胞膜・核間移行によって植物花茎伸長を促進する新規ブラシノステロイド情報伝達因子BIL7の機能解析 (2014)
  • Author(s): 宮地朋子、市川尚斉、松井南、藤岡昭三、長田裕之、浅見忠男、中野雄司
  • Organizer: 植物化学調節学会第49回大会
  • Place of Presentation: 京都府・京都大学
  • Year and Date: 2014-10-18
• [Presentation] イネとシロイヌナズナで異なる反応を示す合成ブラシノステロイドの解析 (2014)
  • Author(s): 中村郁子、藤岡昭三、栃尾尚哉、伊藤晋作、木川隆則、嶋田幸久、松岡信、吉田茂男、木下俊則、浅見忠男、瀬戸秀春、中野雄司
  • Organizer: 日本植物学会第78回大会
  • Place of Presentation: 神奈川県・明治大学
  • Year and Date: 2014-09-13
• [Presentation] Evolution of brassinosteroid biosynthetic pathways: comparative studies on Selaginella and Arabidopsis (2012)
  • Author(s): Ji Nyeong Cheon
  • Organizer: IPMB 2012
  • Place of Presentation: Jeju, South Korea
• [Presentation] ブラシノステロイド情報伝達因子BIL4の細胞内局在と機能解析 (2011)
  • Author(s): 山上あゆみ
  • Organizer: 植物化学調節学会第46回大会
  • Place of Presentation: 宇都宮大(宇都宮市)
  • Year and Date: 2011-11-01
• [Presentation] Brassinosteroids control sex determination in maize (2011)
  • Author(s): Burkhard Schulz
  • Organizer: Plant Biology 2011
  • Place of Presentation: Minneapolis, USA
• [Presentation] ブラシノステロイド情報伝達因子BIL7の核移行条件と相互作用因子の解析
  • Author(s): 宮地朋子、市川尚斉、松井南、藤岡昭三、長田裕之、浅見忠男、中野雄司
  • Organizer: 植物化学調節学会第48回大会
  • Place of Presentation: 新潟大学（新潟県）
• [Presentation] シュートの重力屈性位置決定に対するブラシノステロイドの役割
  • Author(s): 山崎千秋、立山巴、藤岡昭三、中村郁子、嶋田幸久
  • Organizer: 第55回日本植物生理学会年会
  • Place of Presentation: 富山大学（富山県）
• [Presentation] 花茎伸長を促進するブラシノステロイド情報伝達因子BIL7のBIN2、BSU1、 BIL1との相互作用機構の解析
  • Author(s): 宮地朋子、市川尚斉、松井南、藤岡昭三、長田裕之、浅見忠男、中野雄司
  • Organizer: 第55回日本植物生理学会年会
  • Place of Presentation: 富山大学（富山県）
• [Presentation] 葉面積増大に関わるブラシノステロイド情報伝達因子BIL8の細胞内機能の解析
  • Author(s): 中田元基、山上あゆみ、市川尚斉、松井南、藤岡昭三、長田裕之、久城哲夫、浅見忠男、中野雄司
  • Organizer: 第55回日本植物生理学会年会
  • Place of Presentation: 富山大学（富山県）
• [Presentation] ブラシノステロイド情報伝達因子特異的阻害剤化合物のケミカルスクリーニングによるBIL4の機能解析
  • Author(s): 山上あゆみ
  • Organizer: 植物化学調節学会第47回大会
  • Place of Presentation: 山形大（山形県）
• [Presentation] シトクロムP450酵素CYP90A1はブラシノステロイドC-3位酸化酵素である
  • Author(s): 大西利幸
  • Organizer: 植物化学調節学会第47回大会
  • Place of Presentation: 山形大（山形県）
• [Presentation] シロイヌナズナ由来シトクロムP450酵素CYP90A1の酵素学的解析
  • Author(s): 大西利幸
  • Organizer: 第54回日本植物生理学会年会
  • Place of Presentation: 岡山大（岡山県）