Molecular analysis of skin cell activation by orally administered functional food substances

• Project/Area Number: 23380072
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Food science
• Research Institution: The University of Tokyo
• Principal Investigator: SHIMIZU Makoto 東京大学, 農学生命科学研究科, 教授 (30114507)
• Co-Investigator(Kenkyū-buntansha): TOTSUKA Mamoru 東京大学, 大学院・農学生命科学研究科, 准教授 (70227601) ; SATSU Hideo 東京大学, 大学院・農学生命科学研究科, 助教 (80323484)
• Project Period (FY): 2011-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥19,110,000 (Direct Cost: ¥14,700,000、Indirect Cost: ¥4,410,000); Fiscal Year 2013: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2012: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2011: ¥10,010,000 (Direct Cost: ¥7,700,000、Indirect Cost: ¥2,310,000)
• Keywords: 皮膚 / セラミド / コラーゲン / 遺伝子発現 / 繊維芽細胞 / 角化細胞 / 共培養 / スフィンゴシン / DNAマイクロアレイ

Research Abstract

Gene expression changes induced in damaged mice skin by Mg-deficient diet or UV irradiation were elucidated by DNA microarray and PCR analyses. Oral administration of ECM components, such as ceramide and collagen, was found to attenuate the gene expression changes. In vitro experiments using a fibroblast-keratinocyte co-culture system constructed with mouse primary skin cells or human skin cell lines showed that metabolites of ceramide as well as collagen peptides induced similar effects on gene expression of skin cells. These results suggest that ECM components are digested and/or metabolized after oral administration, reach the skin tissue, and then affect keratinocyte functions via modulating fibroblast-keratinocyte interactions.

Research Products

• [Journal Article] Effects of oral administration of glucosylceramide on gene expression changes in hairless mouse skin : Comparison between whole skin, epidermis, and dermis (2013)
  • Author(s): Takatori, R., Lan Phuong, L.V., Iwamoto, T., Satsu, H., Totsuka, M., Shimizu, M
  • Journal Title: Bioscience, Biotechnology, and Biochemistry Volume: 77 Pages: 1882-1887
• [Journal Article] Effects of Oral Administration of Glucosylceramide on Gene Expression Changes in Hairless Mouse Skin: Comparison of Whole Skin, Epidermis, and Dermis (2013)
  • Author(s): Takatori, R., Lan Phuong, L.V., Iwamoto, T., Satsu, H., Totsuka, M., Shimizu, M.
  • Journal Title: Bioscience, Biotechnology, and Biochemistry Volume: 77 Issue: 9 Pages: 1882-1887
  • DOI: 10.1271/bbb.130318
  • NAID: 10031202739
  • ISSN: 0916-8451, 1347-6947
  • Peer Reviewed
• [Presentation] 共培養系においてセラミドが皮膚細胞に及ぼす影響 (2014)
  • Author(s): 赤木 裕, レ ヴ ラン フォン, 薩 秀夫, 戸塚 護, 千田和広, 清水 誠
  • Organizer: 日本農芸化学会2014年度大会
  • Place of Presentation: 川崎
• [Presentation] 共培養系においてセラミドが皮膚細胞に及ぼす影響 (2014)
  • Author(s): 赤木 裕、レ ヴ ラン フォン、薩 秀夫、戸塚 護、千田 和広、清水 誠
  • Organizer: 日本農芸化学会2014年度大会
  • Place of Presentation: 明治大学（神奈川県川崎市）
• [Presentation] Organotypic coculture of mouse skin cells as a model for studying the effects of collagen peptide (2013)
  • Author(s): Phuong, L.V., Takatori. R., Iwamoto,T., Sato, K., Totsuka,M., Satsu, H., Chida,K., Shimizu, M
  • Organizer: 日本農芸化学会2013年度大会
  • Place of Presentation: 仙台
• [Presentation] Organotypic coculture of mouse skin cells as a model for studying the effects of collagen peptide (2013)
  • Author(s): Phuong, L.V
  • Organizer: 2013年度日本農芸化学会大会
  • Place of Presentation: 仙台
• [Presentation] Effects of prolyl-hydroxyproline, a collagen peptide, on gene expression of mouse skin cells in coculture (2012)
  • Author(s): Phuong, L.V., Takatori. R., Iwamoto,T., Sato, K., Totsuka,M., Satsu, H., Chida,K., Shimizu, M
  • Organizer: International Meeting of the Japanese Association for Animal Cell Technology (JAACT2012)
  • Place of Presentation: Nagoya
• [Presentation] Effects of prolyl-hydroxyproline, a collagen peptide, on gene expression of mouse skin cells in co-culture (2012)
  • Author(s): Phuong, L.V
  • Organizer: International Meeting of the Japanese Association for Animal Cell Technology (JAACT2012)
  • Place of Presentation: 名古屋