| Project/Area Number |
23380074
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Food science
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| Research Institution | Niigata University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
FUJIMURA Shinobu 新潟大学, 自然科学系, 准教授 (20282999)
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| Co-Investigator(Renkei-kenkyūsha) |
KATO Hisanori 東京大学, 総括プロジェクト機構, 特任教授 (40211164)
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥18,980,000 (Direct Cost: ¥14,600,000、Indirect Cost: ¥4,380,000)
Fiscal Year 2013: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2012: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2011: ¥9,230,000 (Direct Cost: ¥7,100,000、Indirect Cost: ¥2,130,000)
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| Keywords | オートファジー / アミノ酸 / 肝細胞 / LC3 / アルギニン / 活性酸素種 / サーチュイン / 消化管 / エイジング / マイクロアレイ / 栄養学 / 細胞・組織 |
|---|
| Research Abstract |
Autophagy is a ubiquitous, intracellular system to degrade and recycle cellular components. The nutritional regulation of autophagy was investigated in rat hepatoma H4-II-E cells and gastrointestinal tracts. Especially, the mechanism of regulation, various signaling mechanisms of each amino acid was proved to be ROS-dependent and -independent. Arginine uses NO pathway and mTOR-independent. The level of mRNA of LC3 molecule, an autophagy marker, and related Atg genes regulated by amino acids was discovered. Anti-oxidative food components, epigallocathecin gallate and resveratrol, stimulated autophagy. Finally, the relationship of amino acid regulation of autophagy with sirtuins, an anti-aging gene, were investigated.
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