Identification, exhaustive analysis, clarification of action mechanism and efficient modification of novel hypolipidemic peptides
Project/Area Number |
23380075
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Food science
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Research Institution | Gifu University |
Principal Investigator |
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Co-Investigator(Kenkyū-buntansha) |
HONDA Hiroyuki 名古屋大学, 大学院・工学系研究科, 教授 (70209328)
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Project Period (FY) |
2011-04-01 – 2014-03-31
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Project Status |
Completed (Fiscal Year 2013)
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Budget Amount *help |
¥19,240,000 (Direct Cost: ¥14,800,000、Indirect Cost: ¥4,440,000)
Fiscal Year 2013: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2012: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2011: ¥13,000,000 (Direct Cost: ¥10,000,000、Indirect Cost: ¥3,000,000)
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Keywords | コレステロール / ペプチド / ミセル / ペプチドアレイ / 胆汁酸 / 食品機能 / ABCA1 / ラクトスタチン / Caco-2 / 動脈硬化 / 大豆ペプチド / ソイスタチン |
Research Abstract |
We used the luciferase plasmid containing the cholesterol transporter, intestinal ABCA1 gene promoter to clarify the molecular mechanism of the inhibition of intestinal cholesterol absorption by lactostatin in Caco-2 cells. Our results suggested that PPAR/LXR responsive element related to the suppression of cholesterol absorption by lactostatin in Caco-2 cells. An oleosin having a hypocholesterolemic action was investigated to clarify the active sequence of its induced a hypocholesterolemic action by an exhaustive analysis using a peptide array. We found two novel bile acid binding peptides. Two novel bile acid binding peptides exhibited the greater inhibition of micellar solubility of cholesterol in vitro compared with a bile acid binding peptide VAWWMY as a positive control. We found that QEK derived from soybean beta-conglycinin alpha prime subunit exhibited the significant increase in CYP7A1 mRNA levels compared to the control group in HepG2 cells.
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Report
(4 results)
Research Products
(28 results)
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[Journal Article] Screening of peptides with a high affinity to bile acids using peptide arrays and a computational analysis2011
Author(s)
Takeshita, T., Okochi, M., Kato, R., Kaga, C., Tomita, Y., Nagaoka, S. and Honda, H
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Journal Title
J. Biosci. Bioeng
Volume: 112
Pages: 92-97
NAID
Related Report
Peer Reviewed
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