Identification of food component which selectively inhibits abnormal vascular contraction

• Project/Area Number: 23380077
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Food science
• Research Institution: Yamaguchi University
• Principal Investigator: KOBAYASHI Sei 山口大学, 医学(系)研究科(研究院), 教授 (80225515)
• Co-Investigator(Kenkyū-buntansha): KISHI Hiroko 山口大学, 大学院医学系研究科, 准教授 (40359899) ; KAJIYA Katsuko 鹿児島大学, 農学部, 講師 (00379942) ; ZHANG Ying 山口大学, 大学院医学系研究科, 助教 (10711260)
• Project Period (FY): 2011-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥18,980,000 (Direct Cost: ¥14,600,000、Indirect Cost: ¥4,380,000); Fiscal Year 2013: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2012: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2011: ¥9,230,000 (Direct Cost: ¥7,100,000、Indirect Cost: ¥2,130,000)
• Keywords: 病態生理 / 血管攣縮 / 血管異常収縮 / 血管病 / 機能性食品 / 膜ラフト / 食品機能

Outline of Final Research Achievements

The purpose of this study is to clarify the molecular mechanism of abnormal vascular contraction and to identify the food component which selectively inhibit abnormal vascular contraction without affecting the normal contraction.
In this study, we identified the novel signal mediator mediating between Fyn tyrosine kinase and Rho-kinase in the regulation of abnormal vascular contractions.
We identified the food component which selectively inhibit abnormal vascular contraction without affecting the normal contraction. By these results we applied two patents.

Research Products

• [Journal Article] Fatty acid-binding protein 7 regulates function of caveolae in astrocytes through expression of caveolin-1 (2015)
  • Author(s): Yoshiteru Kagawa, Yuki Yasumoto, Kazem Sharifi, Majid Ebrahimi, Ariful Islam, Hirofumi Miyazaki, Yui Yamamoto, Tomoo Sawada, Hiroko Kishi, Sei Kobayashi, Motoko Maekawa, Takeo Yoshikawa, Eiichi Takaki Akira Nakai, Hiroshi Kogo, Toyoshi Fujimoto, Yuji Owada
  • Journal Title: Glia Volume: 63 Pages: 780-794
  • Peer Reviewed
• [Journal Article] Phosphorylation of the Kinase Domain Regulates Autophosphorylation of Myosin IIIA and Its Translocation in Microvilli (2014)
  • Author(s): Byung Chull An, Tsuyoshi Sakai, Shigeru Komaba, Hiroko Kishi, Sei Kobayashi, Jin Young Kim, Reiko Ikebe, Mistuo Ikebe
  • Journal Title: Biochemistry Volume: 53 Issue: 49 Pages: 7835-7845
  • DOI: 10.1021/bi501247z
  • Peer Reviewed
• [Journal Article] Sphingosylphosphorylcholine induces stress fiber formation via activation of Fyn-RhoA-ROCK signaling pathway in fibroblasts (2012)
  • Author(s): Dan xu
  • Journal Title: Cellular Signaling Volume: 24 Issue: 1 Pages: 282-289
  • DOI: 10.1016/j.cellsig.2011.09.013
  • Peer Reviewed
• [Journal Article] 食品成分による血管病予防の新展開 (2012)
  • Author(s): 加治屋勝子、岸博子、高田雄一、張影、木村友彦、宮成健司、萩原弘、小林誠
  • Journal Title: FFIジャーナル Volume: 217 Pages: 284-289
  • NAID: 40019419438
  • Peer Reviewed
• [Presentation] N-terminus of paxillin regulates actin stress fiber formation by binding to the active Fyn (2015)
  • Author(s): Ying Zhang, Hiroko Kishi, Kenji Miyanari, Tomohiko Kimura, Ryodai Takagaki, Bochao Lyu, Sei Kobayashi
  • Organizer: 第120回日本解剖学会総会全国学術集会・第92回日本生理学会大会 合同大会
  • Place of Presentation: 神戸国際会議場・展示場 (神戸市)
  • Year and Date: 2015-03-21 – 2015-03-23
• [Presentation] Discovery of novel Salacia-derived components which specifically inhibit the ROK-mediated Ca2+- sensitization of vascular smooth muscle contraction (2015)
  • Author(s): Kenji Miyanari, Kazutaka Nojimoto, Katsuko Kajiya, Yuichi Takada, Tomohiko Kimura, Ying Zhang, Hiroko Kishi, Sei Kobayashi
  • Organizer: 第120回日本解剖学会総会全国学術集会・第92回日本生理学会大会 合同大会
  • Place of Presentation: 神戸国際会議場・展示場 (神戸市)
  • Year and Date: 2015-03-21 – 2015-03-23
• [Presentation] アクチン・ストレスファイバー形成における、新規シグナル分子パキシリンと活性型Fynチロシンキナーゼ との相互作用の重要性 (2014)
  • Author(s): 張 影、岸 博子、宮成健司、木村友彦、高柿了大、呂 博超、小林 誠
  • Organizer: 第56回日本平滑筋学会総会
  • Place of Presentation: 新横浜プリンスホテル (横浜市)
  • Year and Date: 2014-08-06 – 2014-08-08
• [Presentation] Important role of lipid mediators in molecular mechanisms of abnormal vascular contraction (2012)
  • Author(s): S.Kobayashi
  • Organizer: 第89回日本生理学会
  • Place of Presentation: 長野県松本文化会館(松本)(招待講演)
  • Year and Date: 2012-03-30
• [Patent(Industrial Property Rights)] 血管攣縮抑制剤 (2014)
  • Inventor(s): 小林 誠、高柿了 大、岸 博子、張 影、加治屋勝子、 渡邉和晃
  • Industrial Property Rights Holder: 国立大学法人山 口大学、株式会 社ラフィーネイン ターナショナル
  • Industrial Property Rights Type: 特許
  • Filing Date: 2014-10-14
• [Patent(Industrial Property Rights)] 血管攣縮抑制剤 (2014)
  • Inventor(s): 高松日出子、小 林誠、宮成健司、 岸 博子、他3名
  • Industrial Property Rights Holder: 株式会社タカマ
  • Industrial Property Rights Type: 特許
  • Filing Date: 2014-09-10