| Project/Area Number |
23380083
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Forest science
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| Research Institution | Kyoto University |
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Principal Investigator |
HIRAI Nobuhiro 京都大学, (連合)農学研究科(研究院), 教授 (00165151)
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| Co-Investigator(Kenkyū-buntansha) |
YAMADA Akiyoshi 信州大学, 農学部, 准教授 (10324237)
TANAKA Chihiro 京都大学, 大学院・農学研究科, 教授 (60263133)
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| Research Collaborator |
FUJITA Thoru 京都府農林水産技術センター
YOSHIKAWA Masami 京都府農林水産技術センター
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000)
Fiscal Year 2013: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2012: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2011: ¥10,530,000 (Direct Cost: ¥8,100,000、Indirect Cost: ¥2,430,000)
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| Keywords | 森林生態 / 保護 / 保全 / 菌根 / マツタケ / 抗菌物質 / シュウ酸 / アカマツ |
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| Research Abstract |
Matsutake is a typical ectomycorrhizal fungus associated with roots of red pine, and forms a fairy ring of fruiting bodies. The area of the fairy ring contains active mycorrhizas, and called as shiro. The previous study reported that that the shiro contained an antimicrobial substance. The antimicrobial substance has been remained unknown for 47 years. Our objective is isolation and identification of the antimicrobial substance from the shiro.
The shiro was extracted with 20% methanol, and the extract was purified with ODS and anion exchange resin to give an active fraction. The active fraction gave a crystalline, which was identified as oxalic acid by an X-ray analysis. However, the antimicrobial activity of the active fraction was clearly higher than that of oxalic acid. This means that the active fraction contains another substance which increases the activity of oxalic acid. The NMR and elemental analysis suggested that the substance was an inorganic compound.
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