| Budget Amount *help |
¥18,720,000 (Direct Cost: ¥14,400,000、Indirect Cost: ¥4,320,000)
Fiscal Year 2013: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2012: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2011: ¥8,710,000 (Direct Cost: ¥6,700,000、Indirect Cost: ¥2,010,000)
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| Research Abstract |
The cells were collected from the different stages of lignification or different organs by a laser micro-dissection (LMD) technique and were subjected to the analyses of lignin structure and gene expression.
Transcriptions of the lignification related genes were analyzed by Real-time PCR. COMT, CCoAOMT, and CAD, were transcribed prior to lignin deposition. The transcription levels of CCR and 4CL were lower than them. CCR stands in the lignification specific pathway; therefore it is considered to be a regulatory gene in the lignin biosynthesis. The lignin structure analysis was achieved with only 0.1 square millimeter samples as a minimum requirement by the combined LMD and Py-GC/MS. The results indicated that syringyl lignin is synthesized in the beginning of lignification, and that structural changes of lignification still proceeds even in the area in which lignification seems already completed by the histochemical observations.
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