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Local analysis of wood cell wall formation by using laser microdissection

Research Project

Project/Area Number 23380103
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Wood science
Research InstitutionChiba Institute of Technology

Principal Investigator

WATANABE Ugai  千葉工業大学, 工学部, 准教授 (70337707)

Co-Investigator(Kenkyū-buntansha) ABE Hisashi  独立行政法人森林総合研究所, 木材特性研究領域, 主任研究員 (80343812)
Co-Investigator(Renkei-kenkyūsha) KAWAI Gota  千葉工業大学, 工学部, 教授 (70211860)
Project Period (FY) 2011-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥18,980,000 (Direct Cost: ¥14,600,000、Indirect Cost: ¥4,380,000)
Fiscal Year 2013: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2012: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2011: ¥11,310,000 (Direct Cost: ¥8,700,000、Indirect Cost: ¥2,610,000)
Keywords樹木形成層 / レーザーマイクロダイセクション / 定量PCR解析 / 遺伝子発現解析 / 樹木細胞生理 / 遺伝子 / 森林工学
Research Abstract

In order to clarify the molecular mechanism of cell wall formation in the cambium of tree, the procedures for local sampling of cambial cells and quantitative analysis of the expressed genes were investigated. The cells could be locally collected from the regions of cell division and cell expansion in the cambium. A trace of genes expressed in each region could be extracted and their amounts could be determined with high accuracy. The results obtained indicated that the expression ratio of proteins involved in wood cell wall formation varied within the cambium, which affected the molecular mechanism of cell wall formation.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Annual Research Report
  • 2011 Annual Research Report
  • Research Products

    (11 results)

All 2014 2013 2012 Other

All Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (10 results)

  • [Journal Article] In vitro induction of secondary xylem-like tracheary elements in calli of hybrid poplar (Poplus sieboldii x P. grandidentata)2013

    • Author(s)
      Y. Yamagishi, J. Yoshimoto, H. Uchiyama, E, Nabeshima, S. Nakaba, U. Watanabe, R. Funada
    • Journal Title

      Planta

      Volume: 237 Pages: 1179-1185

    • Related Report
      2013 Final Research Report 2012 Annual Research Report
    • Peer Reviewed
  • [Presentation] LMD・デジタルPCR による樹木形成層発現遺伝子の解析手法の検討2014

    • Author(s)
      渡辺宇外, 安部久, 二村典宏, 篠原健司
    • Organizer
      日本木材学会大会
    • Place of Presentation
      愛媛大学(松山市)
    • Related Report
      2013 Final Research Report
  • [Presentation] 交雑ポプラ培養細胞からの管状要素分化に対する乾燥処理の影響2014

    • Author(s)
      山岸祐介, 北村圭, 吉本靖東, 渡辺宇外, 半智史, 船田良
    • Organizer
      日本木材学会大会
    • Place of Presentation
      愛媛大学(松山市)
    • Related Report
      2013 Final Research Report
  • [Presentation] Mechanical load-induced secondary cell wall formation and its regulation in plant cell2014

    • Author(s)
      U. Watanabe, M. Sekiguchi, K. Kojima, H. Tsujimura, H. Abe
    • Organizer
      IUFRO conference division 5 forest products
    • Place of Presentation
      Estoril Portugal
    • Related Report
      2013 Final Research Report
  • [Presentation] スギのα-チューブリンおよびβ-チューブリンの塩基配列およびアミノ酸配列の解析2014

    • Author(s)
      渡辺宇外, 安部久, 二村典宏, 篠原健司
    • Organizer
      日本木材学会大会
    • Place of Presentation
      北海道大学(札幌市)
    • Related Report
      2013 Final Research Report
  • [Presentation] スギのα-チューブリンおよびβ-チューブリンの塩基配列およびアミノ酸配列の解析2012

    • Author(s)
      渡辺宇外, 他3名
    • Organizer
      日本木材学会
    • Place of Presentation
      北海道大学(札幌市)
    • Year and Date
      2012-03-15
    • Related Report
      2011 Annual Research Report
  • [Presentation] Mechanical load-induced secondary cell wall fromation and its regulation in plant cell2012

    • Author(s)
      Ugai Watanabe
    • Organizer
      2012 IUFRO Conference Division 5 Forest Products
    • Place of Presentation
      Estoril Congress Centre, Lisbon, Portugal
    • Related Report
      2012 Annual Research Report
  • [Presentation] In vitro induction of secondary xylem like tracheary elements from hybrid poplar cultured cells2012

    • Author(s)
      Yusuke Yamagishi
    • Organizer
      2012 IUFRO Conference Division 5 Forest Products
    • Place of Presentation
      Estoril Congress Centre, Lisbon, Portugal
    • Related Report
      2012 Annual Research Report
  • [Presentation] Quality and quantity of DNA in wood stored for long-period after harvesting2012

    • Author(s)
      Hisashi Abe
    • Organizer
      2012 IUFRO Conference Division 5 Forest Products
    • Place of Presentation
      Estoril Congress Centre, Lisbon, Portugal
    • Related Report
      2012 Annual Research Report
  • [Presentation] In vitro induction of secondary xylem like tracheary elements from hybrid poplar cultured cells

    • Author(s)
      Y. Yamagishi, H. Uchiyama, J. Yoshimoto, S. Nakaba, U. Watanabe, R. Funada
    • Organizer
      IUFRO conference division 5 forest products
    • Place of Presentation
      Estoril Portugal
    • Related Report
      2013 Final Research Report
  • [Presentation] LMD・デジタルPCRによる樹木形成層発現遺伝子の解析手法の検討

    • Author(s)
      渡辺宇外、安部久、二村典宏、篠原健司
    • Organizer
      第64回日本木材学会大会
    • Place of Presentation
      愛媛大学(愛媛県)
    • Related Report
      2013 Annual Research Report

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Published: 2011-04-06   Modified: 2019-07-29  

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