Elucidation of mechanism of neuroendocrine control in reproduction for advancement of artificial seed production of commercial bivalve mollusks

• Project/Area Number: 23380109
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General fisheries
• Research Institution: Tohoku University
• Principal Investigator: MAKOTO Osada 東北大学, (連合)農学研究科(研究院), 教授 (30177208)
• Co-Investigator(Kenkyū-buntansha): ITOH Naoki 東北大学, 大学院・農学研究科, 助教 (30502736) ; CHIBA Hiroaki 北里大学, 水産学部, 准教授 (50236816)
• Project Period (FY): 2011-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥18,980,000 (Direct Cost: ¥14,600,000、Indirect Cost: ¥4,380,000); Fiscal Year 2013: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2012: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2011: ¥9,100,000 (Direct Cost: ¥7,000,000、Indirect Cost: ¥2,100,000)
• Keywords: 二枚貝 / 神経内分泌 / エストロゲンシグナリング / 配偶子形成 / 産卵 / エストロゲンシグナリン

Research Abstract

We cloned the cDNA transcripts of prepro Gonadotropin Releasing Hormones (GnRH)-like peptides from the central nervous system of scallop(pyGnRH)and oyster (cgGnRH). The putative pyGnRH peptide stimulated the proliferation of spermatogonia, which was mediated through the induction of estrogen synthesis by pyGnRH.
We successfully cloned two novel forms of estrogen receptor from two kinds of mussels and Japanese scallop. These receptors were classified each into an estrogen receptor (ER) and estrogen-related receptor (ERR) and were expressed in both ovary and testis. The flounder ERa-mediated gene assay suggested that pyER behaves as a transcription factor to estrogen while pyERR is a constitutive activator.
Serotonin (5-HT)-induced oocyte maturation and sperm motility is inhibited by oocyte maturation arresting factor (OMAF) due to interference of extracellular Ca2+ influx. The release from suppressive OMAF due to absorption with antibody against OMAF amplified the 5-HT-induced spawning.

Research Products

• [Journal Article] Roles of extracellular ions and pH in 5-HT-induced sperm motility in marine bivalve (2014)
  • Author(s): S. M. Hadi ALAVI, M. Osada, 他5名
  • Journal Title: Reproduction Volume: 147 Issue: 3 Pages: 331-345
  • DOI: 10.1530/rep-13-0418
  • Peer Reviewed
• [Journal Article] Molluscan GnRH Associated with Reproduction (2013)
  • Author(s): Makoto Osada and Nicholas Treen
  • Journal Title: Gen. Comp. Endocrinol Volume: 181 Pages: 254-258
  • DOI: 10.1016/j.ygcen.2012.09.002
  • Peer Reviewed
• [Journal Article] Isolation and functional characterization for oocyte maturation and sperm motility of the oocyte maturation arresting factor from the Japanese scallop, Patinopecten yessoensis (2012)
  • Author(s): Yuan, Y., Tanabe, T., Maekawa, F., Inaba, K., Maeda, Y., Itoh, N., Takahashi, K.G., Osada, M.
  • Journal Title: Gen Comp Endocrinol Volume: 179 Issue: 3 Pages: 350-357
  • DOI: 10.1016/j.ygcen.2012.09.006
  • Peer Reviewed
• [Journal Article] Mollusc gonadotropin-releasing hormone directly regulates gonadal functions : A primitive endocrine system controlling reproduction (2012)
  • Author(s): N.Treen, K.Tsutsui, et al
  • Journal Title: Gen.Comp.Endocrinol. Volume: (in press) Issue: 2 Pages: 167-172
  • DOI: 10.1016/j.ygcen.2012.01.008
  • Peer Reviewed
• [Presentation] Serotonin induces initiation of sperm motility in marine bivalve mollusks (2013)
  • Author(s): Sayyed Mohammad Hadi Alavi, Natsuki Matsumura, Kogiku Shiba, Naoki Itoh, Keisuke G. Takahashi, Kazuo Inaba, Makoto Osada
  • Organizer: 日本動物学会第84回岡山大会
  • Place of Presentation: 岡山市
• [Presentation] ホタテガイGnRH による生殖細胞発達に関わる遺伝子の網羅的解析の試み (2013)
  • Author(s): 大越葉月, 峰岸有紀, 伊藤直樹, 高橋計介, 尾定誠
  • Organizer: 平成25年度日本水産学会秋季大会
  • Place of Presentation: 津市
• [Presentation] ホタテガイGnRH の性転換と精子形成への関与 (2013)
  • Author(s): 大内仁志, 伊藤直樹, 高橋計介, 尾定誠
  • Organizer: 平成25年度日本水産学会秋季大会
  • Place of Presentation: 津市
• [Presentation] Expression and functional analyses for estrogen receptors of Japanese scallop, Patinopecten yessoensis (2013)
  • Author(s): Yurika Otoki, Emily C. Marquez, Yoko Ohota, Takeshi Kitano, Naoki Itoh, Keisuke G. Takahashi, Makoto Osada
  • Organizer: International Congress of Comparative Endocrinology (17th ICCE2013)
  • Place of Presentation: Barcelona, Spain
• [Presentation] ホタテガイにおけるエストロゲン受容体の発現様式と機能 (2013)
  • Author(s): 乙木百合香, Emily Marquez, 太田陽子, 北野健, 伊藤直樹, 高橋計介, 尾定誠
  • Organizer: 平成25年度日本水産学会春季大会
  • Place of Presentation: 東京都品川区
• [Presentation] ホタテガイGnRHによる生殖細胞発達に関わる遺伝子の網羅的解析の試み (2013)
  • Author(s): 大越葉月、他4名
  • Organizer: 平成25年度日本水産学会秋季大会
  • Place of Presentation: 津市
• [Presentation] ホタテガイGnRHの性転換と精子形成への関与 (2013)
  • Author(s): 大内仁志、他3名
  • Organizer: 平成25年度日本水産学会秋季大会
  • Place of Presentation: 津市
• [Presentation] Expression and functional analyses for estrogen receptors of Japanese scallop, Patinopecten yessoensis (2013)
  • Author(s): Yurika Otoki、他6名
  • Organizer: International Congress of Comparative Endocrinology (17th ICCE2013)
  • Place of Presentation: バルセロナ、スペイン
• [Presentation] ホタテガイにおけるエストロゲン受容体の発現様式と機能 (2013)
  • Author(s): 乙木百合香
  • Organizer: 平成25年度日本水産学会春季大会
  • Place of Presentation: 東京
• [Presentation] Diversity in the Mechanisms of Sperm Activation in Fish and Bivalve (2012)
  • Author(s): S. M. Hadi Alavi, Natsuki Matsumura, Naoki Itoh, Keisuke G. Takahashi and Makoto Osada
  • Organizer: 平成24年精子研究会
  • Place of Presentation: 仙台市
  • Year and Date: 2012-11-10
  • Invited
• [Presentation] 海産二枚貝エストロゲン受容体の機能解析 (2012)
  • Author(s): 乙木百合香, Emily Marquez, 伊藤直樹, 高橋計介, 尾定誠
  • Organizer: 2012年度日本動物学会中部支部大会
  • Place of Presentation: 松本市
• [Presentation] ホタテガイエストロゲン受容体遺伝子の発現解析 (2012)
  • Author(s): 乙木百合香, Emily Marquez, 伊藤直樹, 高橋計介, 尾定誠
  • Organizer: 平成24年度日本水産学会秋季大会
  • Place of Presentation: 下関市
• [Presentation] CALCIUM-DEPENDNET SPERM ACTIVATION IN BIVALVE MOLLUSC IS PROHIBITED BY SCALLOP OOCYTE MATURATION ARRESTING FACTOR AND ENHANCED BY SEROTONIN (2012)
  • Author(s): S. M. Hadi Alavi, Natsuki Matsumura, Naoki Itoh, Kogiku Shiba, Kazuo Inaba, Makoto Osada
  • Organizer: AQUA 2012
  • Place of Presentation: Prague, Czech Republic
• [Presentation] Molluscan GnRH associated with reproduction (2012)
  • Author(s): Makoto Osada
  • Organizer: The Seventh Congress of the Asia and Oceania Society for Comparative Endocrinology (AOSCE)
  • Place of Presentation: Kuala Lumpur, Malaysia
  • Invited
• [Presentation] Mollusc gonadotropin-releasing hormone directly regulates gonadal functions : a primitive endocrine system controlling reproduction (2012)
  • Author(s): Treen N., Itoh N., Miura H., Kikuchi I., Ueda T., Takahashi K.G., Ubuka T., Yamamoto K., Sharp P.J., Tsutsui K., Osada M
  • Organizer: The Seventh Congress of the Asia and Oceania Society for Comparative Endocrinology (AOSCE)
  • Place of Presentation: Kuala Lumpur, Malaysia
• [Presentation] Primary structural analyais of estrogen receptors in marine bivalves, The Seventh Congress of the Asia and Oceania Society for Comparative Endocrinology (AOSCE) (2012)
  • Author(s): Otoki Y., Itoh N., Treen N., Marquez E., Osada M
  • Organizer: Kuala Lumpur
  • Place of Presentation: Malaysia
• [Presentation] ホタテガイエストロゲン受容体遺伝子の発現解析 (2012)
  • Author(s): 乙木百合香
  • Organizer: 平成24年度日本水産学会秋季大会
  • Place of Presentation: 下関
• [Presentation] Diversity in the Mechanisms of Sperm Activation in Fish and Bivalve (2012)
  • Author(s): S. M. Hadi Alavi
  • Organizer: 平成24年精子研究会
  • Place of Presentation: 仙台
  • Invited
• [Presentation] 海産二枚貝エストロゲン受容体の機能解析 (2012)
  • Author(s): 乙木百合香
  • Organizer: 2012年度日本動物学会中部支部大会
  • Place of Presentation: 松本
• [Presentation] CALCIUM-DEPENDNET SPERM ACTIVATION IN BIVALVEMOLLUSC IS PROHIBITED BY SCALLOP OOCYTE MATURATION ARRESTING FACTOR AND ENHANCED BY SEROTONIN (2012)
  • Author(s): S. M. Hadi Alavi
  • Organizer: AQUA 2012
  • Place of Presentation: チェコ共和国プラハ
• [Presentation] ホタテガイ由来卵成熟休止因子OMAFの精子運動抑制調節へのCa^<2+>の関与 (2012)
  • Author(s): 松村夏希, 他8名
  • Organizer: 平成24年度日本水産学会春季大会
  • Place of Presentation: 東京
• [Presentation] Molluscan GnRH associated with reproduction (2012)
  • Author(s): Makoto Osada
  • Organizer: The Seventh Congress of the Asia and Oceania Society for Comparative Endocrinology
  • Place of Presentation: クアラルンプール/マレーシア(招待講演)
• [Presentation] Mollusc gonadotropin-releasing hormone directly regulates gonadal functions : a primitive endocrine system controlling reproduction (2012)
  • Author(s): Treen N, 他10名
  • Organizer: The Seventh Congress of the Asia and Oceania Society for Comparative Endocrinology
  • Place of Presentation: クアラルンプール/マレーシア
• [Presentation] Primary structural analyais of estrogen receptors in marine bivalves (2012)
  • Author(s): Otoki Y, 他4名
  • Organizer: The Seventh Congress of the Asia and Oceania Society for Comparative Endocrinology
  • Place of Presentation: クアラルンプール/マレーシア
• [Presentation] Bivalve Mollusc Gonadotropin-Releasing Hormone (2011)
  • Author(s): Nicholas Treen, Naoki Itoh, Hanae Miura, Ippei Kikuchi, Takenori Ueda, Keisuke G.Takahashi , Takayoshi Ubuka , Kazutoshi Yamamoto , Kazuyoshi Tsutsui , Peter Sharp , Makoto Osada
  • Organizer: 第36回日本比較内分泌学会大会
  • Place of Presentation: 東京都千代田区
• [Presentation] 海産二枚貝のエストロゲンレセプターの一次構造 (2011)
  • Author(s): 乙木百合香, 伊藤直樹, Nicholas Treen, 尾定誠
  • Organizer: 第36回日本比較内分泌学会大会
  • Place of Presentation: 東京都千代田区
• [Presentation] ホタテガイ由来卵成熟休止因子OMAF の精子運動抑制調節へのCa^<2+>の関与 (2011)
  • Author(s): 松村夏希, Hadi Alavi, 伊藤直樹, 高橋計介, 尾定誠, 加藤元一, 寺井しま, 稲葉一男, 柴小菊
  • Organizer: 平成24年度日本水産学会春季大会
  • Place of Presentation: 東京都品川区
• [Presentation] Bivalve Mollusc Gonadotropin-Releasing Hormone (2011)
  • Author(s): Nicholas Treen, 他10名
  • Organizer: 第36回日本比較内分泌学会大会
  • Place of Presentation: 東京
• [Presentation] 海産二枚貝のエストロゲンレセプターの一次構造 (2011)
  • Author(s): 乙木百合香, 他3名
  • Organizer: 第36回日本比較肉分泌学会大会
  • Place of Presentation: 東京
• [Remarks]
  • URL: http://www.agri.tohoku.ac.jp/zoshoku/seisyoku.html