Molecular mechanism of egg-sperm interaction during fertilization in teleost and development of application system for artificial fertilization

• Project/Area Number: 23380110
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Aquatic life science
• Research Institution: Ehime University
• Principal Investigator: MATSUBARA Takahiro 愛媛大学, 南予水産研究センター, 教授 (60443389)
• Co-Investigator(Kenkyū-buntansha): MIURA Takeshi 愛媛大学, 南予水産研究センター, 教授 (00261339) ; OHTA Kohei 愛媛大学, 南予水産研究センター, 准教授 (10585764)
• Co-Investigator(Renkei-kenkyūsha): MOCHIDA Kazuhiko 水産総合研究センター, 瀬戸内海区水産研究所, グループ長 (00371964) ; SAWAGUCHI Sayumi 水産総合研究センター, 西海区水産研究所, 研究員 (40553087) ; MIURA Chiemi 愛媛大学, 南予水産研究センター, 講師 (90518002)
• Project Period (FY): 2011-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000); Fiscal Year 2014: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2013: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2012: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2011: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
• Keywords: 硬骨魚類 / 受精 / 精子活性化 / 精子誘導 / 卵門 / 卵巣腔液

Outline of Final Research Achievements

Teleost egg is covered with a thick envelope called chorion, and has a micropyle at animal pole through which sperm access to the egg plasma membrane. The present study elucidated the egg-sperm interaction being consisted of activation of sperm motility by an ovarian fluid coupled with sperm guiding into micropyle by a protein factor. Such fertilization system is suggested to be common in many teleost species.

Research Products

• [Journal Article] Sperm Attractant in the Micropyle Region of Fish and Insect Eggs (2013)
  • Author(s): Ryuzo Yanagimachi, Gary Cherr, Takahiro Matsubara, Tadashi Andoh, Tatsuo Harumi, Carol Vines, Murali Pillai, Frederick Griffin, Hajime Matsubara, Tina Weatherby, and Kenneth Kaneshiro
  • Journal Title: BIOLOGY OF REPRODUCTION Volume: 88(2):47 Pages: 1-11
  • DOI: 10.1095/biolreprod.112.105072
  • Peer Reviewed
• [Journal Article] Characterization of vitellogenin and its derived yolk proteins in cloudy catshark(Scyliorhinus torazame). (2013)
  • Author(s): Yamane K, Yagai T, Nishimiya O, Sugawara R, Amano H, Fujita T, Hiramatsu N, Todo T, Matsubara T, Hara A.
  • Journal Title: Fish Physiol Biochem Volume: 39(2) Pages: 373-390
  • DOI: 10.1007/s10695-012-9706-1
  • Peer Reviewed
• [Journal Article] 「カットスロートトラウトOncorhynchus clarkiにおける２型ビテロジェニン転写産物および蛋白質の卵発達に伴う発現変化」 (2013)
  • Author(s): 莚平裕次、水田紘子、Wenshu LUO、盛田祐加、澤口小有美、松原孝博、平松尚志、東藤孝、原彰彦
  • Journal Title: 日本水産学会誌 Volume: 79(2) Pages: 175-189
  • NAID: 10031138611
  • Peer Reviewed
• [Presentation] 海産魚卵顕微注入法の開発と評価 (2014)
  • Author(s): 後藤理恵・松原孝博・柳 蓉沄・山羽悦郎・山内晧平・長濱嘉孝
  • Organizer: 平成26年度日本水産学会秋季大会
  • Place of Presentation: Olhao、Portugal
  • Year and Date: 2014-09-19 – 2014-09-22
• [Presentation] VERY LOW-DENSITY LIPOPROTEIN IS PRIMARY CARRIER OF NEUTRAL LIPIDS TO OOPLASM LIPID DROPLETS IN TELEOSTS (2014)
  • Author(s): Ryu, Y.-W., Todo, T., Hiramatsu, N., Matsubara, T., Sullivan, C.V. and Hara, A.
  • Organizer: 10th International Symposium on Reproductive Physiology of Fish
  • Place of Presentation: Olhao、Portugal
  • Year and Date: 2014-05-25 – 2014-05-30
• [Presentation] OVARIAN YOLK FORMATION IN FISHES: MOLECULAR MECHANISMS UNDERLYING FORMATION OF LIPID DROPLETS AND VITELLOGENINDERIVED YOLK PROTEINS (2014)
  • Author(s): Hiramatsu, N., Todo, T., Sullivan, C.V., Reading, B.J., Matsubara, T., Ryu, Y.-W., Mizuta, H., Luo, W.,Nishimiya, O., Wu, M., Mushirobira, Y. and Hara, A.
  • Organizer: 10th International Symposium on Reproductive Physiology of Fish
  • Place of Presentation: Olhao、Portugal
  • Year and Date: 2014-05-25 – 2014-05-30
• [Presentation] VISUALIZATION AND MIGRATION OF PRIMORDIAL GERM CELLS IN A MARINE FISH, THE JAPANESE ANCHOVY, ENGRAULIS JAPONICAS (2014)
  • Author(s): Goto, R., Ryu, Y.-W., Kurita, K., Matsubara, T., Yamauchi, K. and Nagahama, Y.
  • Organizer: 10th International Symposium on Reproductive Physiology of Fish
  • Place of Presentation: Olhao、Portugal
  • Year and Date: 2014-05-25 – 2014-05-30
• [Presentation] カツオー本釣り用カタクチイワシの養殖に向けたシラス期からの飼育技術の開発 (2011)
  • Author(s): 田中真誠, 松原孝博
  • Organizer: 日本水産学会
  • Place of Presentation: 長崎
  • Year and Date: 2011-09-30
• [Book] マグロの資源と生物学 2.2成熟 (2014)
  • Author(s): 松原孝博、玄浩一郎、澤口小有美、持田和彦、二階堂英城
  • Total Pages: 294
  • Publisher: 成山堂書店