Protectivity of extensive infections by a bacterial outer membrane protein glyceraldehyde-3-phosphate dehydrogenase (GAPDH)

• Project/Area Number: 23380111
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General fisheries
• Research Institution: Ehime University
• Principal Investigator: KAWAI Kenji 愛媛大学, (連合)農学研究科(研究院), 教授 (60127925)
• Co-Investigator(Kenkyū-buntansha): OSHIMA Syun-ichirou 高知大学, 教育研究部総合科学系, 教授 (80325406) ; IMAJOH Masayuki 高知大学, 教育研究部自然科学系, 講師 (20565741)
• Project Period (FY): 2011-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥13,390,000 (Direct Cost: ¥10,300,000、Indirect Cost: ¥3,090,000); Fiscal Year 2013: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2012: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2011: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
• Keywords: 魚病 / ワクチン / 外膜タンパク質 / GAPDH / Edwardsiella tarda / 免疫 / 抗原 / ヒト病原体

Research Abstract

Protective effects of an outer membrane protein glyceraldehyde-3-phosphate dehydrogenase (GAPDH) from a bacterium Edwardsiella tarada against other difficult fish diseases, pseudotuberculosis and nocardiosis, was investigated using yellowtail (Seriola quinqueradiata). The fish immunized with GAPDH did not show enough level of protection against both diseases. However, it showed the growth inhibition of infecting Nocardia inside fish body, increased the production of immunoglobulin, and stimulated some immuno-competent genes. Fish pathogenic Mycobacterium sp. was found to be a model bacterium that may show pathogenicity to humans.

Research Products

• [Journal Article] Genotypic characteristics of a Mycobacterium sp. isolated from yellowtail Seriola quinqueradiata and striped jack Pseudocaranx dentex in Japan (2013)
  • Author(s): Masayuki Imajoh, Hidehiro Sugiura, Yumiko Hashida, Kishio Hatai, Syun-ichirou Oshima, Masanori Daibata, Kenji Kawai
  • Journal Title: Microbiology and Immunology Volume: 57巻 Pages: 13-20
  • NAID: 40019554352
  • Peer Reviewed
• [Journal Article] Genotypic characteristics of a Mycobacterium sp. iseolated from yellowtail Seriola quiqueradiata and striped jack Pseudocaranx dentex in Japan (2013)
  • Author(s): Masayuki Imajoh
  • Journal Title: Microbiology and Immunology Volume: 57 Issue: 1 Pages: 13-20
  • DOI: 10.1111/j.1348-0421.2012.00514.x
  • Peer Reviewed
• [Presentation] ブリのノカルジア症に対するEdwardsiella tarda GAPDH 組換えサブユニットおよびDNAワクチンの防御効果について (2014)
  • Author(s): 田村一樹
  • Organizer: 平成26年度日本魚病学会春季大会
  • Place of Presentation: 函館国際ホテル
  • Year and Date: 2014-03-30
• [Presentation] ブリのノカルジア症に対するEdwardsiella tarda GAPDH組換えサブユニットおよびDNAワクチンの防御効果について (2014)
  • Author(s): 田村一樹・大黒司貴・福本陽一・藤岡博哉・山根仁・山本剛・大嶋俊一郎・ 川合研児・今城雅之
  • Organizer: 平成26年度日本魚病学会春季大会
  • Place of Presentation: 函館国際ホテル北海道函館市大手町5-10
• [Remarks] 愛媛大学HP 研究者一覧から業績のページ
  • URL: http://kenqweb.office.ehime-u.ac.jp/Profiles/0012/0003343/theses1.html