| Project/Area Number |
23380117
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Aquatic bioproduction science
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| Research Institution | Nihon University |
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Principal Investigator |
MORI Tsukasa 日本大学, 生物資源科学部, 教授 (60241379)
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| Co-Investigator(Kenkyū-buntansha) |
SEKI Taiichro 日本大学, 生物資源科学部, 教授 (20187834)
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| Co-Investigator(Renkei-kenkyūsha) |
ARAKI Kazuo 独立行政法人水産総合研究センター, その他部局等, 研究員 (00202739)
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| Project Period (FY) |
2011-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥18,720,000 (Direct Cost: ¥14,400,000、Indirect Cost: ¥4,320,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2013: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2012: ¥8,320,000 (Direct Cost: ¥6,400,000、Indirect Cost: ¥1,920,000)
Fiscal Year 2011: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
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| Keywords | 遺伝子組換え / GH（成長ホルモン） / アマゴ / 発現遺伝子 / 代謝産物 / GH transgenic amago / 脂肪酸解析 / 大規模遺伝子解析 / ＧＨ遺伝子組換え / GH transgenic Amago / アゼチジン2-カルボン酸 / ＴＯＦＭＳ / GH遺伝子組み換え魚 / iTRAQ / 比較定量トランスクリプトーム / プロテオーム解析 |
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| Outline of Final Research Achievements |
GH transgenic Amago were compared the FAs composition and content in the liver using gas chromatography. Eleven kinds of FA were detected. The composition of SFA and MUFA such as 14:0, 16:1n-7, and cis-18:1n-7 was significantly decreased in GH transgenic Amago. On the other hand, the composition of PUFAs such as 18:2n-6, 20:4n-6, and 22:5n-3 was significantly increased. Levels of serum glucose and triacylglycerol were significantly decreased in the GH transgenics compared with +/+ fish. Furthermore, 3’-tag digital gene expression profiling was performed using liver tissues from Tg/Tg and +/+ fish, and showed that Mid1ip1, which is an important factor to activate ACC, was down-regulated in Tg/Tg fish, while genes involved in FA catabolism were up-regulated, including ACSL1 and ACOX3. These data suggest that liver tissue from GH transgenic Amago showed starvation by alteration in glucose and lipid metabolism due to GH overexpression.
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