| Budget Amount *help |
¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000)
Fiscal Year 2014: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2013: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2012: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2011: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
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| Outline of Final Research Achievements |
We analyzed disease resistance trait and developed the physical map of flounder. The streptococcul infection disease resistant flounder increased monocyte and granulocyte dramatically after infection. One the other side, the native immunity system of streptococcul infection sensitive flounder did not respond against streptococcul infection. We detected TRL21 and NKEF genes in the region of linkage group 10 contributed to streptococcul infection disease resistance trail by detailing flounder physical map. And, we analyzed the sequences of TRL21 and NKEF genomic genes of the resistance, sensitive and famed flounder. We found single nucleotide polymorphism in regulatory site of TRL21 gene of resistance flounder and deletion between ninth exon and intron of TRL21 in sensitive flounder.
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