| Budget Amount *help |
¥18,850,000 (Direct Cost: ¥14,500,000、Indirect Cost: ¥4,350,000)
Fiscal Year 2013: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2012: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2011: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
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| Research Abstract |
Marine fishes are generally unable to produce sufficient eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA). It is therefore necessary to supplement cultured marine fish species diets with fish oils to supply EPA and DHA. Given that freshwater fishes can synthesize both EPA and DHA, they presumably express all of the enzymes required for this pathway. Therefore, we used marine fish, nibe croaker to produce a transgenic line carrying the elongase gene isolated from masu salmon. Fatty acid analysis revealed that the liver EPA content in the transgenic fish was lower. However, docosapentaenoic acid content in the transgenic fish was 2.28-fold higher than in non-transgenic fish. Further, tetracosapentaenoic acid was specifically detected in the transgenic fish. We therefore conclude that the development of transgenic fish lines with these fatty acid-metabolizing enzymes could be a powerful tool for manipulating fatty acid metabolic pathways in fish.
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