Determination of optimum conditions for growing symbiotic dinoflagellates, Amphidinium species, and development of methods for its rapid mass culture

• Project/Area Number: 23380151
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Agricultural environmental engineering
• Research Institution: Osaka Prefecture University
• Principal Investigator: KITAYA Yoshiaki 大阪府立大学, 生命環境科学研究科(系), 教授 (60169886)
• Co-Investigator(Kenkyū-buntansha): ENDO Ryosuke 大阪府立大学, 大学院・生命環境科学研究科, 助教 (10409146)
• Project Period (FY): 2011-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥19,110,000 (Direct Cost: ¥14,700,000、Indirect Cost: ¥4,410,000); Fiscal Year 2013: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2012: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2011: ¥10,920,000 (Direct Cost: ¥8,400,000、Indirect Cost: ¥2,520,000)
• Keywords: 生物環境調節 / 生物生産システム / 渦鞭毛藻 / 培養 / 藻類培養 / 藻類培養システム / 微細藻 / CO2 / O2 / 光合成 / 比増殖速度

Research Abstract

Symbiotic dinoflagellates, Amphidinium species, are expected to be pharmaceutically useful microalgae, because they produce antitumor macrolides. A microalgae production system with a large number of cells at a high density has been developed to produce macrolide compounds efficiently. In this study, effects of culture conditions on the cellular multiplication of dinoflagellates were investigated to determine the optimum culture conditions for obtaining high yield of the microalgae. The maximum specific growth rate was obtained at a temperature of 26oC, PPFDs of0.05-0.07 mmol m-2 s-1, photoperiod of 12 h d-1, O2 concentration of 5% and CO2 concentration of 0.1%. The results demonstrate that Amphidinium species could efficiently multiply under the condition with relatively low light intensities and low O2 concentrations with additional inorganic nutrients.The optimal depth of cuilture solution was 10 mm.

Research Products

• [Journal Article] Selection of microalgae suitable for culturing with digestate from methane fermentation (2013)
  • Author(s): Khanh, N., Kitaya, Y., Xiao, L., Endo, R., Shibuya, T
  • Journal Title: Environmental Technology Volume: Vol.34 Pages: 2039-2045
  • Peer Reviewed
• [Journal Article] Selection of microalgae suitable for culturing with digestate from methane fermentation (2013)
  • Author(s): Khanh, N., Kitaya, Y., Xiao, L., Endo, R., Shibuya, T.
  • Journal Title: Environmental Technology Volume: 34 Issue: 13-14 Pages: 2039-2045
  • DOI: 10.1080/09593330.2013.828093
  • Peer Reviewed
• [Presentation] Culture of microalgae with digested sludge from methane fermentation (2014)
  • Author(s): Khanh, N., Kitaya, Y., Xiao, L., Endo, R., Shibuya, T
  • Organizer: Annual Conference of Eco-Engineering
  • Place of Presentation: Numazu, Japan
  • Year and Date: 2014-06-28
• [Presentation] An effective utilization of digested sludge from methane fermentation with food and agricultural wastes for microalgae culture (2012)
  • Author(s): Khanh, N., Kitaya, Y., Xiao, L., Endo, R., Shibuya, T
  • Organizer: 8th Asia Pacific Conference on Algal Biotechnology and 1st Conference on Coastal Biotechnology
  • Place of Presentation: Adelaide, Australia
  • Year and Date: 2012-07-11
• [Presentation] Effects of O_2 and CO_2 concentrations on cellular multiplication and net photosynthetic rates of dinoflagellates, Amphidinium species (2012)
  • Author(s): Kitaya, Y., Xiao, L
  • Organizer: 8th Asia Pacific Conference on Algal Biotechnology and 1st Conference on Coastal Biotechnology
  • Place of Presentation: Adelaide, Australia
  • Year and Date: 2012-07-09
• [Presentation] Effects of O2 and CO2 concentrations on cellular multiplication and net photosynthetic rates of dinoflagellates, Amphidinium species (2012)
  • Author(s): Y. Kitaya, L. Xiao
  • Organizer: 8th Asia-Pacific Conference on Algal Biotechnology
  • Place of Presentation: Aderade, Australia