| Project/Area Number |
23380151
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Agricultural environmental engineering
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| Research Institution | Osaka Prefecture University |
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Principal Investigator |
KITAYA Yoshiaki 大阪府立大学, 生命環境科学研究科(系), 教授 (60169886)
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| Co-Investigator(Kenkyū-buntansha) |
ENDO Ryosuke 大阪府立大学, 大学院・生命環境科学研究科, 助教 (10409146)
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥19,110,000 (Direct Cost: ¥14,700,000、Indirect Cost: ¥4,410,000)
Fiscal Year 2013: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2012: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2011: ¥10,920,000 (Direct Cost: ¥8,400,000、Indirect Cost: ¥2,520,000)
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| Keywords | 生物環境調節 / 生物生産システム / 渦鞭毛藻 / 培養 / 藻類培養 / 藻類培養システム / 微細藻 / CO2 / O2 / 光合成 / 比増殖速度 |
|---|
| Research Abstract |
Symbiotic dinoflagellates, Amphidinium species, are expected to be pharmaceutically useful microalgae, because they produce antitumor macrolides. A microalgae production system with a large number of cells at a high density has been developed to produce macrolide compounds efficiently. In this study, effects of culture conditions on the cellular multiplication of dinoflagellates were investigated to determine the optimum culture conditions for obtaining high yield of the microalgae. The maximum specific growth rate was obtained at a temperature of 26oC, PPFDs of0.05-0.07 mmol m-2 s-1, photoperiod of 12 h d-1, O2 concentration of 5% and CO2 concentration of 0.1%. The results demonstrate that Amphidinium species could efficiently multiply under the condition with relatively low light intensities and low O2 concentrations with additional inorganic nutrients.The optimal depth of cuilture solution was 10 mm.
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