Expressions of myogenetic genes through DNA demethylation are triggered by muscle contraction in skeletal muscle.
| Project/Area Number |
23380159
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Zootechnical science/Grassland science
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| Research Institution | Kyushu University |
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Principal Investigator |
YOSHIHIDE Ikeuchi 九州大学, (連合)農学研究科(研究院), 教授 (90168112)
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| Co-Investigator(Kenkyū-buntansha) |
水野 谷航 (水野谷 航) 九州大学, 大学院農学研究院 (20404056)
佐藤 祐介 宇都宮大学, 農学部, 助教 (50589520)
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| Co-Investigator(Renkei-kenkyūsha) |
佐藤 祐介 宇都宮大学, 農学部 (50589520)
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥18,330,000 (Direct Cost: ¥14,100,000、Indirect Cost: ¥4,230,000)
Fiscal Year 2013: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2012: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
Fiscal Year 2011: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
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| Keywords | APOBEC2 / 骨格筋 / 筋形成 / 筋分化 / DNA脱メチル化 / 食肉生産 / 筋萎縮 / DNA 脱メチル化 / 筋収縮 / 筋芽細胞 / 筋管 / ミトコンドリア / オートファジー |
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| Outline of Final Research Achievements |
Understanding the mechanism of myogenesis makes it possible to manipulate the muscle characteristics (the size of muscle, muscle fiber types etc.) and leads to the contribution to the fields of meat production, medicine and sport science. To evaluate APOBEC2 function in skeletal muscle, we performed histological, immunohistochemical and electron microscopic observations and gene expression analysis. The following results were obtained: 1. APOBEC2 (putative DNA/RNA editing enzyme) migrates to z-bands of sarcomere during myotube formation phase. 2. APOBEC2-deficient mice have the degenerating fibers, rimmed vacuoles (RVs) and enlarged mitochondria. 3. Satellite cells from APOBEC2-deficient mice enter into the differentiation phase early, as compared with those from wild-type mice. Also, the injured muscles of APOBEC2- deficient mice showed early regeneration.
The function of APOBEC2 in muscle is still unknown, but it is likely that APOBEC2 is responsible for myogenesis.
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Report
(4 results)
Research Products
(61 results)
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[Journal Article] Thiobencarb Herbicide Reduces Growth, Photosynthetic Activity, and Amount of Rieske Iron-Sulfur Protein in the Diatom Thalassiosira pseudonana.2013
Author(s)
Shimasaki Y, Tsuyama M, Tasmin R, Qiu X, Shimizu M, Sato Y, Yamasaki Y, Kato-Unoki Y, Nukata A, Nakashima T, Ichinose H, Wariishi H, Honjo T, Oshima Y
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Journal Title
Journal of biochemical and molecular toxicology
Volume: 27
Issue: 9
Pages: 437-444
DOI
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Peer Reviewed
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[Journal Article] Effect of 48-h Food Deprivation on the Expressions of Myosin Heavy-Chain Isoforms and Fiber Type-Related Factors in Rats2013
Author(s)
Mizunoya, W., Sawano, S., Iwamoto, Y., Sato, Y., Tatsumi, R. and Ikeuchi, Y.
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Journal Title
Journal of Nutritional Science and Vitaminology
Volume: 59
Issue: 4
Pages: 289-298
DOI
NAID
ISSN
0301-4800, 1881-7742
Related Report
Peer Reviewed
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[Journal Article] Growth factor regulation of neural chemorepellent Sema3A expression in satellite cell cultures2011
Author(s)
Do, M.-K. Q., Sato, Y., Shimizu, N., Suzuki, T., Shono, J.-I., Mizunoya, W., Nakamura, M., Ikeuchi, Y., Anderson, J. E., and Tatsumi, R
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Journal Title
American Journal of Physiology-Cell Physiology
Volume: 301
Issue: 5
Pages: C1270-C1279
DOI
Related Report
Peer Reviewed
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[Presentation] Eicosapentaenoic Acid Affects Skeletal Muscle Fiber Types relating factors via PPARδ Pathway in Isolated Fibers of Rats2013
Author(s)
Wataru Mizunoya, Yusuke Komiya, Judy E. Anderson, Tsuyoshi Goto, Nobuyuki Takahashi, Teruo Kawada, Mako Nakamura, Ryuichi Tatsumi, Yoshihide Ikeuchi
Organizer
Keystone Symposia, Nuclear Receptors and Friends: Roles in Energy Homeostasis and Metabolic Dysfunction
Place of Presentation
Alpbach Congress Centrum, Alpbach
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[Presentation] 筋衛星細胞の活性化における物理刺激受容機構の解明2011
Author(s)
原美菜子, 田畑久仁子, 真鍋宣隆, 佐藤祐介, 水野谷航, 中村真子, 辰巳隆一, 西村正太郎, 田畑正志, 池内義秀
Organizer
第114回日本畜産学会大会
Place of Presentation
北里大学獣医学部十和田キャンパス(十和田市)
Year and Date
2011-08-26
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