| Project/Area Number |
23380162
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied animal science
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| Research Institution | Iwate University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
KIZAKI Keiichiro 岩手大学, 農学部, 准教授 (40337994)
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000)
Fiscal Year 2013: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2012: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2011: ¥10,530,000 (Direct Cost: ¥8,100,000、Indirect Cost: ¥2,430,000)
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| Keywords | 細胞・組織 / 遺伝子 / ゲノム / マイクロアレイ / 発生・分化 |
|---|
| Research Abstract |
Trophoblastic cells, derived from the outer layer of morula, are key cell for implantation and placentation. Bovine trophoblastic cells contain two specific cells; mononucleate and binucleate, and they express and produce various specific molecules, individually. In this study, we examined the mechanism of trophoblastic cell lineage, namely morphological and functional differentiation, using previously established cell lines. Matrigel, which derived from extra-cellular matrix, effectively induced trophoblastic cell differentiation. The bi-nucleation associated with increasing expression of retro-element. The differentiated cells produced retro-element molecule, fematrin-1, which was named by fetomaternal trinucleate cell inducer 1, and it, has a fusion activity between trophoblastic cells and endometrial cells in bovine. These results showed a direct evidence for cell-to-cell fusion in bovine placenta and open a new sight for bovine reproduction, especially early gestation.
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