Epigenetic analysis of zygotic gene activation by maternal factor

• Project/Area Number: 23380164
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied animal science
• Research Institution: Kyoto University
• Principal Investigator: MINAMI Naojiro 京都大学, (連合)農学研究科(研究院), 准教授 (30212236)
• Co-Investigator(Kenkyū-buntansha): MATSUMOTO Kazuya 近畿大学, 生物理工学部, 教授 (20298938) ; TSUKAMOTO Satoshi 放射線医学総合研究所, 主任研究員 (80510693)
• Project Period (FY): 2011-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥8,320,000 (Direct Cost: ¥6,400,000、Indirect Cost: ¥1,920,000); Fiscal Year 2013: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2012: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
• Keywords: マウス初期胚 / エピジェネティクス / 胚性ゲノムの活性化 / 遺伝子発現 / 母性因子 / siRNA / 多能性 / 分化 / Smyd3 / Epigenetics / mouse embryo / development / Oct4 / Cdx2 / 受精卵 / ヒストンメチル化 / Chd1 / タンパク質分解 / ユビキチンプロテアソーム / リソソーム / マウス / 初期胚 / クロマチンリモデリング

Outline of Final Research Achievements

Smyd3 is a histone H3 lysine 4 (H3K4) di- and tri-methyltransferase that forms a transcriptional complex with RNA polymerase II and CHD1, which recognizes trimethylated histone H3 lysine 4, is a protein belonging to the family of ATPase-dependent chromatin remodeling factors. In the present study, we investigated the effects of RNA interference (RNAi)-mediated repression on the development of mouse embryos. In Smyd3-, and Chd1-knockdown embryos, the percentage of inner cell mass (ICM)-derived colony formation and trophectoderm (TE)-derived cell attachment was significantly decreased, resulting in a reduction in the number of viable offspring. Furthermore, the expression of Pou5f1, Nanog, and Cdx2, was dramatically decreased in both Smyd3- and Chd1-knockdown embryos. Moreover, in Chd1-knockdown embryos, expression of Hmgpi, which regulate Pou5f1, Nanog, and Cdx2, was also significantly suppressed at zygotic gene activation (ZGA).

Research Products

• [Journal Article] Histone methyltransferase Smyd3 regulates early embryonic lineage commitment in the mouse (2015)
  • Author(s): Suzuki S, Nozawa Y, Tsukamoto S, Kaneko T, Imai S, Minami N.
  • Journal Title: Reproduction Volume: 印刷中
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] A hyperactive <i>piggyBac</i> transposon system is an easy-to-implement method for introducing foreign genes into mouse preimplantation embryos (2015)
  • Author(s): Suzuki S, Tsukiyama T, Kaneko T, Imai S, Minami N.
  • Journal Title: Journal of Reproduction and Development Volume: 61 Issue: 3 Pages: 241-244
  • DOI: 10.1262/jrd.2014-157
  • NAID: 130005077740
  • ISSN: 0916-8818, 1348-4400
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Fluorescence-based visualization of autophagic activity predicts mouse embryo viability (2014)
  • Author(s): Satoshi Tsukamoto S, Hara T, Yamamoto A, Kito S, Minami N, Kubota T, Sato K, Kokubo T
  • Journal Title: Scientific Reports Volume: 4 Issue: 1 Pages: 4533-4533
  • DOI: 10.1038/srep04533
  • Peer Reviewed / Open Access
• [Journal Article] Functional Analysis of Lysosomes During Mouse Preimplantation Embryo Development (2013)
  • Author(s): Tsukamoto S
  • Journal Title: Journal of Reproduction and Development Volume: 59 Pages: 33-39
  • NAID: 10031156809
  • Peer Reviewed
• [Journal Article] Mouse zygote-specific proteasome assembly chaperone important for maternal-to-zygotic transition (2013)
  • Author(s): Shin SW
  • Journal Title: Biolology Open Volume: 2 Issue: 2 Pages: 170-182
  • DOI: 10.1242/bio.20123020
  • Peer Reviewed
• [Journal Article] Functional Analysis of Nocturnin, a Circadian Deadenylase, at Maternal- to-zygotic Transition in Mice. (2013)
  • Author(s): Nishikawa S
  • Journal Title: Journal of Reproduction and Development Volume: in press
  • Peer Reviewed
• [Journal Article] Donor cells at the gl phase enhance homogeneous gene expression among blastomeres in bovine somatic cell nuclear transfer embryos (2012)
  • Author(s): Iwamoto D, Kasamatsu A, Ideta A, Urakawa M, Matsumoto K, Hosoi Y, Iritani A, Aoyagi Y, Saeki K
  • Journal Title: Cell Reprogram Volume: 14 Pages: 20-28
  • Peer Reviewed
• [Journal Article] Identification and characterization of an oocyte factor required for development of porcine nuclear transfer embryos (2011)
  • Author(s): Miyamoto, K., Nagai, K., Kitamura, N., Nishikawa, T., Ikegami, H., Binh, N., Tsukamoto, S., Matsumoto, M., Tsukiyama, T., Minami, N., Yamada, M., Ariga, H., Miyake, M., Kawarasaki, T., Matsumoto, K., Imai, H.
  • Journal Title: Proc. Natl. Acad. Sci. USA Volume: 108 Issue: 17 Pages: 7040-7045
  • DOI: 10.1073/pnas.1013634108
  • Peer Reviewed
• [Journal Article] 卵母細胞特異的に発現するOoglについて (2011)
  • Author(s): 南直治郎
  • Journal Title: 卵子学(森嵩英総編集) Pages: 803-805
• [Presentation] クロマチンリモデリング因子Chd1は胚性ゲノムの活性化を通じて初期胚の最初の分化に関与する (2014)
  • Author(s): 鈴木 伸之介、野澤 佑介、金子 武人、今井 裕、南 直治郎
  • Organizer: 第37回日本分子生物学会大会
  • Place of Presentation: 横浜市
  • Year and Date: 2014-11-25 – 2014-11-27
• [Presentation] CHD1 is the zygotic determinant of Oct4 and Cdx2 expression via the Hmgpi pathway during preimplantation development (2014)
  • Author(s): Suzuki, S, Nozawa Y, Tsukamoto S, Kaneko T, Imai H, Minami N.
  • Organizer: 2014 World Congress of Reproductive Biology
  • Place of Presentation: Edinburgh, UK
  • Year and Date: 2014-09-02 – 2014-09-04
• [Presentation] クロマチンリモデリング因子Chd1は胚性ゲノムの活性化を通じて初期胚の最初の分化に関与する研究 (2014)
  • Author(s): 鈴木 伸之介、野澤 佑介、金子 武人、今井 裕、南 直治郎
  • Organizer: 第107回日本繁殖生物学会大会
  • Place of Presentation: 帯広市
  • Year and Date: 2014-08-20 – 2014-08-24
• [Presentation] マウス初期胚におけるクロマチン再構成タンパク質Chd1に関する研究 (2013)
  • Author(s): 鈴木 伸之介、野澤 佑介、金子 武人、今井 裕、南 直治郎
  • Organizer: 第106回日本繁殖生物学会大会、2013/ 9/11-14、東京
  • Place of Presentation: 東京
  • Year and Date: 2013-09-11 – 2013-09-14
• [Presentation] マウス初期胚におけるヒストンメチル化酵素Smyd3の役割について (2013)
  • Author(s): 野澤 佑介、鈴木 伸之介、塚本 智史、金子 武人、今井 裕、南 直治郎
  • Organizer: 第106回日本繁殖生物学会大会、2013/ 9/11-14、東京
  • Place of Presentation: 東京
  • Year and Date: 2013-09-11 – 2013-09-14
• [Presentation] Ing3 has an important role during oocyte maturation in the mouse. (2013)
  • Author(s): Suzuki S, Nozawa Y, Tsukamoto S, Kaneko T, Imai H, Minami N.
  • Organizer: The 46th Annual Meeting of the Society for the Study of Reproduction
  • Place of Presentation: Montreal, Canada
  • Year and Date: 2013-07-22 – 2013-07-26
• [Presentation] マウス卵母細胞の成熟過程におけるIng3の役割 (2013)
  • Author(s): 鈴木 伸之介、野澤 佑介、金子 武人、今井 裕、南 直治郎
  • Organizer: 第54回日本哺乳動物卵子学会
  • Place of Presentation: 東京
  • Year and Date: 2013-05-25 – 2013-05-26
• [Presentation] Effects of chromatin remodeling factor Chd1 on in vitro differentiation of mouse preimplantation embryos (2012)
  • Author(s): Suzuki S, Minami N
  • Organizer: Joint Meeting of the Brithish Society for Cell Biology, Developmental Biology and the Japanese Society for Developmental Biologists
  • Place of Presentation: Worwick, England
• [Presentation] Effects of chromatin remodeling factor Chd1 on in vitro differentiation of mouse preimplantation embryos (2012)
  • Author(s): Suzuki S, Minami N
  • Organizer: 10th Annual Meeting of International Society for Stem Cell Research
  • Place of Presentation: Yokohama, Japan
• [Presentation] Oog1 promoter controls the meiosis-specific gene expression in germ cells (2012)
  • Author(s): Ishida M, Minami N
  • Organizer: The 45th Annual Meeting of the Society for the Study of Reproduction
  • Place of Presentation: State Colledge, USA
• [Presentation] Regulation of meiosis-specific gene expression by the promoter of an oocyte-specific gene, Oog1 (2012)
  • Author(s): Minami N
  • Organizer: The 9th Annual Conference of the Asian Reproductive Biotechnology Society
  • Place of Presentation: Manila, Philippines
  • Invited
• [Presentation] Meiosis-specific gene expression by the promoter of an oocyte-specific gene, Oog1 (2012)
  • Author(s): Minami N
  • Organizer: 2012 CABX Workshop and International Symposium
  • Place of Presentation: Jeju, Korea
  • Invited
• [Presentation] マウス初期胚におけるクロマチン再構成タンパク質Chd1に関する研究 (2012)
  • Author(s): 鈴木 伸之介、南 直治郎
  • Organizer: 第105回日本繁殖生物学会大会
  • Place of Presentation: つくば市
• [Presentation] マウス初期胚におけるヒストンメチル化酵素Smyd3の役割について (2012)
  • Author(s): 野澤 佑介、南 直治郎
  • Organizer: 第105回日本繁殖生物学会大会
  • Place of Presentation: つくば市
• [Presentation] マウス初期胚におけるクロマチン再構成タンパク質Chd1の役割 (2012)
  • Author(s): 鈴木 伸之介、南 直治郎
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡市
• [Presentation] The functional analysis of Chdl in mouse oocyte maturation using lipid-siRNA complexes (2011)
  • Author(s): Suzuki S, Tsukamoto S, Ohta Y, Imai H, Minami N
  • Organizer: 3^<rd> World Congress of Animal Reproduction
  • Place of Presentation: ケアンズ(オーストラリア)
  • Year and Date: 2011-10-10
• [Presentation] マウス卵母細胞特異的遺伝子Ooglのプロモーター解析 (2011)
  • Author(s): 石田未弥・岡崎永里子・塚本智史・木村康二・太田有紀・鬼頭靖司・今井裕・南直治郎
  • Organizer: 第104回日本繁殖生物学会
  • Place of Presentation: いわて県民情報交流センター(盛岡市)
  • Year and Date: 2011-09-15
• [Presentation] Identification of Oogl promoter regions in mouse growing oocytes (2011)
  • Author(s): Ishida M, Okazaki E, Tsukamoto S, Kimura K, Ohota Y, Kito S, Imai H, Minami N
  • Organizer: 44th Society for the Study of Reproduction
  • Place of Presentation: ポートランド(アメリカ合衆国)
  • Year and Date: 2011-08-02
• [Presentation] トランスフェクションを用いたマウス卵母細胞中でのChdlの機能解析 (2011)
  • Author(s): 鈴木伸之・塚本智史・太田有紀・今井裕・南直治郎
  • Organizer: 第5回エビジェネティクス研究会年会
  • Place of Presentation: KKRホテル熊本(熊本市)
  • Year and Date: 2011-05-19