| Project/Area Number |
23380167
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied animal science
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| Research Institution | Kochi University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
KASAI Magosaburo 高知大学, 教育研究部総合科学系, 教授 (60152617)
KOSHIMOTO Chihiro 宮崎大学, フロンティア科学実験総合センター, 教授 (70295210)
MATSUKAWA Kazutsugu 高知大学, 教育研究部総合科学系, 准教授 (00532160)
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥17,940,000 (Direct Cost: ¥13,800,000、Indirect Cost: ¥4,140,000)
Fiscal Year 2013: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2012: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2011: ¥10,140,000 (Direct Cost: ¥7,800,000、Indirect Cost: ¥2,340,000)
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| Keywords | 繁殖 / 凍結保存 / 卵子 / アクアポリン / mPTP / MPTP / 細胞内氷晶形成 / ミトコンドリア |
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| Research Abstract |
To increase the survival of mouse oocytes after vitrification, we first tried to increase the permeability of their inner mitochondrial membrane. When oocytes were treated with phenylarsine oxide to open the mitochondrial permeability transition pore, a non-specific channel present in the inner membrane, the survival of vitrified oocytes increased significantly just after warming. However, they were dead after 1 h of culture. Then, we tried to increase the expression of water channels, which are expressed in the inner membrane. However, the tolerance to vitrification was not improved, probably because the expression was not increased. Next, we cryopreserved oocytes by equilibrium vitrification to promote dehydration/concentration of the cell and mitochondria. The survival was high even if they were warmed in the air, suggesting that they were vitrified under a near equilibrium state. These results suggest that equilibrium vitrification is suitable for cryopreservation of mouse oocytes.
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