The improvement of the tolerance of mammalian oocytes to cryopreservation by inducing endogenous expression of water/cryoprotectant channels or by gating the channels
| Project/Area Number |
23380167
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied animal science
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| Research Institution | Kochi University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
KASAI Magosaburo 高知大学, 教育研究部総合科学系, 教授 (60152617)
KOSHIMOTO Chihiro 宮崎大学, フロンティア科学実験総合センター, 教授 (70295210)
MATSUKAWA Kazutsugu 高知大学, 教育研究部総合科学系, 准教授 (00532160)
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥17,940,000 (Direct Cost: ¥13,800,000、Indirect Cost: ¥4,140,000)
Fiscal Year 2013: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2012: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2011: ¥10,140,000 (Direct Cost: ¥7,800,000、Indirect Cost: ¥2,340,000)
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| Keywords | 繁殖 / 凍結保存 / 卵子 / アクアポリン / mPTP / MPTP / 細胞内氷晶形成 / ミトコンドリア |
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| Research Abstract |
To increase the survival of mouse oocytes after vitrification, we first tried to increase the permeability of their inner mitochondrial membrane. When oocytes were treated with phenylarsine oxide to open the mitochondrial permeability transition pore, a non-specific channel present in the inner membrane, the survival of vitrified oocytes increased significantly just after warming. However, they were dead after 1 h of culture. Then, we tried to increase the expression of water channels, which are expressed in the inner membrane. However, the tolerance to vitrification was not improved, probably because the expression was not increased. Next, we cryopreserved oocytes by equilibrium vitrification to promote dehydration/concentration of the cell and mitochondria. The survival was high even if they were warmed in the air, suggesting that they were vitrified under a near equilibrium state. These results suggest that equilibrium vitrification is suitable for cryopreservation of mouse oocytes.
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Report
(4 results)
Research Products
(78 results)
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[Journal Article] Increased metabolite levels of glycolysis and pentose phosphate pathway in rabbit atherosclerotic arteries and hypoxic macrophage2014
Author(s)
Atsushi Yamashita, Yan Zhao, Yunosuke Matsuura, Kazuaki Yamasaki, Sayaka Moriguchi-Goto, Chihiro Sugita, Takashi Iwakiri, Nozomi Okuyama, Chihiro Koshimoto, Keiichi Kawai, Nagara Tamaki, Songji Zhao, Yuji Kuge, Yujiro Asada
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Journal Title
PLos One
Volume: 9
Issue: 1
Pages: e86426-e86426
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Effects of Cholesterol-Loaded Cyclodextrins on the Rate and the Quality of Motility in Frozen and Thawed Rabbit Sperm2014
Author(s)
Kazutoshi Nishijima, Shinji Yamaguchi, Mai Tanaka, Yusuke Sakai, Chihiro Koshimoto, Masatoshi Morimoto, TeruoWatanabe, Jianglin Fan, Shuji Kitajima
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Journal Title
Experimental Animals
Volume: 63
Issue: 2
Pages: 149-154
DOI
NAID
ISSN
0007-5124, 1341-1357, 1881-7122
Related Report
Peer Reviewed / Open Access
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[Journal Article] Rapid movement of water and cryoprotectants in pig expanded blastocysts via channel processes : its relevance to their higher tolerance to cryopreservation2013
Author(s)
Bo Jin, Ryu-ichi Higashiyama, Yu-ichi Nakata, Jun-ichi Yonezawa, Shangdan Xu, Masashi Miyake, Sei-ichi Takahashi, Kazuhiro Kikuch, Ken-ichi Yazawa, Shuhei Mizobuchi, Saori Niimi, Mizuho Kitayama, Chihiro Koshimoto, Kazutsugu Matsukawa, Magosaburo Kasai, Keisuke Edashige
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Journal Title
Biology of Reproduction
Volume: 89
Issue: 4
Pages: 1-12
DOI
Related Report
Peer Reviewed
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[Journal Article] Arterial <sup>18</sup>F-Fluorodeoxyglucose Uptake Reflects Balloon Catheter-Induced Thrombus Formation and Tissue Factor Expression via Nuclear Factor-κB in Rabbit Atherosclerotic Lesions2013
Author(s)
Atsushi Yamashita, Yan Zhao, Songji Zhao, Yunosuke Matsuura), Chihiro Sugita, Takashi Iwakiri, Nozomi Okuyama, Kazuyo Ohe, Chihiro Koshimoto, Keiichi Kawai, Nagara Tamaki, Yuji Kuge, Yujiro Asada.
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Journal Title
Circulation Journal
Volume: 77
Issue: 10
Pages: 2626-2635
DOI
NAID
ISSN
1346-9843, 1347-4820
Related Report
Peer Reviewed
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[Presentation] ^<18>F-FDG uptake reflects thrombus formation and tissue factor expression via nuclear factor-κB in rabbit atherosclerotic lesions2012
Author(s)
Atsushi Yamashita, Yan Zhao, Songji Zhao, Yunosuke Matsuura, Chihiro Sugita, Takashi Iwakiri, Kazuyo Ohe, Chihiro Koshimoto, Keiichi Kawai, Nagara Tamaki, Yuji Kuge, Yujiro Asada
Organizer
American Heart Association, Scientific Session
Place of Presentation
JW Marriott Los Angeles at LA Live, Los Angeles, California
Related Report
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[Presentation] The role of aquaporin 9 in the movement of DMSO and acetamide in mouse morulae2012
Author(s)
Keisuke Edashige, Shinsuke Seki, Hayato Arimura, Kan Matsuo, Yu-ichi Nakata, Bo Jin, Kazutsugu Matsukawa, Chihiro Koshimoto, Magosaburo Kasai
Organizer
SSR's 45th Annual Meeting of Society of the Study of Reprocution
Place of Presentation
The Alumni Hall at the Hetzel Union Building, The Pennsylvania State University, University Park, Pennsylvania
Related Report
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[Presentation] The role of aquaporin 9 in the movement of DMSO and acetamide in mouse morulae.2012
Author(s)
Keisuke Edashige, Shinsuke Seki, Hayato Arimura, Kan Matsuo, Yu-ichi Nakata, Bo Jin, Kazutsugu Matsukawa, Chihiro Koshimoto, Magosaburo Kasai.
Organizer
45th Annual Meeting of Society for the Study of Reprocution
Place of Presentation
The Alumni Hall at the Hetzel Union Building, The Pennsylvania State University, (Pennsylvania, USA)
Related Report
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[Presentation] 18F-FDG uptake reflects thrombus formation and tissue factor expression via nuclear factor-κB in rabbit atherosclerotic lesions.2012
Author(s)
A Yamashita, Y Zhao, S Zhao, Y Matsuura, C Sugita, T Iwakiri, K Ohe, C Koshimoto, K Kawai, N Tamaki, Y Kuge, Y Asada.
Organizer
American Heart Association, Scientific Session
Place of Presentation
JW Marriott Los Angeles at LA Live (California, USA)
Related Report
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[Presentation] オキナワトゲネズミTokudaia muenninkiの2009年調査以降に確認された新たな生息地2011
Author(s)
山田文雄, 中田勝士, 村田知慧, 木戸文香, 黒岩麻里, 城ヶ原貴通, 越本知大, 三谷匡, 阿部愼太郎, 河内紀浩, 小高信彦, 高嶋敦史, 安村茂樹
Organizer
第48回沖縄生物学会大会・第47回日本動物分類学会大会合同大会
Place of Presentation
琉球大学西原キャンパス,沖縄県中頭郡西原町
Related Report
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[Presentation] 沖縄島北部におけるオキナワトゲネズミTokudaia muenninkiの2009年調査以降に確認された新たな生息地2011
Author(s)
山田文雄, 中田勝士, 村田知慧, 木戸文香, 黒岩麻里, 城ヶ原貴通, 越本知大, 三谷匡, 阿部愼太郎, 河内紀浩, 小高信彦, 高嶋敦史
Organizer
日本哺乳類学会2011年度大会
Place of Presentation
宮崎市民プラザ&宮崎観光ホテル,宮崎市
Related Report
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