The remodeling of the endometrium, which supports embryo implantation

• Project/Area Number: 23380172
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Basic veterinary science/Basic zootechnical science
• Research Institution: Nagoya University
• Principal Investigator: HONDO Eiichi 名古屋大学, 生命農学研究科, 教授 (30271745)
• Co-Investigator(Kenkyū-buntansha): INOUE Naoko 名古屋大学, 大学院・生命農学研究科, 助教 (90377789)
• Project Period (FY): 2011-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥18,460,000 (Direct Cost: ¥14,200,000、Indirect Cost: ¥4,260,000); Fiscal Year 2013: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2012: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2011: ¥8,710,000 (Direct Cost: ¥6,700,000、Indirect Cost: ¥2,010,000)
• Keywords: 子宮内膜間質細胞 / エストロジェン / 分化・脱分化 / 幹細胞 / 胚着床 / 子宮内膜 / 間質細胞 / マウス / 組織再構築

Research Abstract

The mechanism of differentiation and dedifferentiation of the uterine stromal cells was investigated. In the murine reproductive cycle, estrogen and progesterone regulates them in the complicated manner. This study clarified that especially estrogen are strongly involved in this mechanism. LIF, Oct3/4, and Nanog proteins were up-regulated from postestrus to proestrus, on the other hand, estrogen solely increase the expression Klf4 mRNA, which regulates Oct3/4 directly.

Research Products

• [Journal Article] The contribution of leukemia inhibitory factor (LIF) for embryo implantation differs among strains of mice (2014)
  • Author(s): Kobayashi R, Terakawa J, Kato Y, Azimi S, Inoue N, Ohmori Y, Hondo E
  • Journal Title: Immunobiology Volume: (in press)
  • Peer Reviewed
• [Journal Article] The contribution of leukemia inhibitory factor (LIF) for embryoimplantation differs among strains of mice. (2014)
  • Author(s): Ryosuke Kobayashi, Jumpei Terakawa, Yasumasa Kato, Shafiqullah Azimi, Naoko Inoue, Yasushige Ohmori, Eiichi Hondo
  • Journal Title: Immunobiology Volume: in press
  • Peer Reviewed
• [Journal Article] The complete control of murine pregnancy from embryo implantation to parturition (2012)
  • Author(s): Terakawa J, Watanabe T, Obara R, Sugiyama M, Inoue N, Ohmori Y, Hosaka YZ, Hondo E
  • Journal Title: Reproduction Volume: 143(3) Pages: 411-5
  • Peer Reviewed
• [Journal Article] The complete control of murine pregnancy from embryo implantation to parturition (2012)
  • Author(s): Terakawa J, et al
  • Journal Title: Reproduction Volume: 143 Pages: 411-415
  • Peer Reviewed
• [Journal Article] Kisspeptin neurons mediate reflex ovulation in the musk shrew (Suncus murinus) (2011)
  • Author(s): Inoue N, Sasagawa K, Ikai K, Sasaki Y, Tomikawa J, Oishi S, Fujii N, Uenoyama Y, Ohmori Y, Yamamoto N, Hondo E, Maeda K, Tsukamura H
  • Journal Title: Proc Natl Acad Sci U S A Volume: 108(42) Pages: 17527-32
  • Peer Reviewed
• [Journal Article] Embryo implantation is blocked by intraperitoneal injection with anti-LIF antibody in mice (2011)
  • Author(s): Terakawa J, Wakitani S, Sugiyama M, Inoue N, Ohmori Y, Kiso Y, Hosaka YZ, Hondo E
  • Journal Title: J Reprod Dev Volume: 57(6) Pages: 700-7
  • NAID: 10030406178
  • Peer Reviewed
• [Journal Article] Kisspeptin neurons mediate reflex ovulation in the musk shrew (Suncus murinus) (2011)
  • Author(s): Inoue N, et al
  • Journal Title: Proc Natl Acad Sci Volume: 108 Pages: 17527-32
  • Peer Reviewed
• [Journal Article] Embryo implantation is blocked by intraperitoneal injection with anti-LIF antibody in mice (2011)
  • Author(s): Terakawa J, et al
  • Journal Title: J Reprod Dev Volume: 57 Pages: 700-707
  • Peer Reviewed
• [Presentation] In vivo エレクトロポレーション法を用いたマウス子宮内膜への遺伝子導入 (2013)
  • Author(s): 小林・早川・井上・大森・本道
  • Organizer: 日本畜産学会
  • Place of Presentation: 新潟大学
  • Year and Date: 2013-09-09
• [Presentation] インターロイキン6(IL-6)ファミリーの相互補完によるマウス胚着床の制御 (2013)
  • Author(s): 小林・大森・井上・寺川・本道
  • Organizer: 日本獣医学会学術集会
  • Place of Presentation: 東京大学
  • Year and Date: 2013-03-29
• [Presentation] In vivoエレクトロポレーション法を用いたマウス子宮内膜への遺伝子導入 (2013)
  • Author(s): 小林良祐、早川靖彦、井上直子、大森保成、本道栄一
  • Organizer: 日本畜産学会
  • Place of Presentation: 新潟大学五十嵐キャンパス
• [Presentation] インターロイキン６ (IL-6) ファミリーの相互補完によるマウス胚着床の制御 (2013)
  • Author(s): 小林 良祐 (本道栄一）
  • Organizer: 第155回日本獣医学会学術集会
  • Place of Presentation: 東京
• [Presentation] Role of LIF/STAT3 signaling pathway for maintenance of the endometrium in non-pregnant mice (2012)
  • Author(s): Suga/Terakawa/Sugiyama/Ohmori/Inoue/Hondo
  • Organizer: The 4th Congress of Asian Association of Veterinary Anatomist
  • Place of Presentation: Phuket, Thailand
  • Year and Date: 2012-10-25
• [Presentation] The regulation of embryo spacing by Ihh, BMPs and Wnts in murine uterus (2012)
  • Author(s): Kobayashi/Terakawa/Ohmori/Inoue/Hondo
  • Organizer: The 4th Congress of Asian Association of Veterinary Anatomist
  • Place of Presentation: Phuket, Thailand
  • Year and Date: 2012-10-25
• [Presentation] Involvement of Cxcl1 and Olfm1 in murine embryo implantation (2012)
  • Author(s): Nii/Terakawa/Ohmori/Inoue/Hondo
  • Organizer: The 4th Congress of Asian Association of Veterinary Anatomist
  • Place of Presentation: Phuket, Thailand
  • Year and Date: 2012-10-25
• [Presentation] The effect of IL-11 and CNTF on embryo implantation (2012)
  • Author(s): Tajima/Terakawa/Ohmori/Inoue/Hondo
  • Organizer: The 4th Congress of Asian Association of Veterinary Anatomist
  • Place of Presentation: Phuket, Thailand
  • Year and Date: 2012-10-25
• [Presentation] 非妊娠マウスにおける子宮内膜の形態変化への白血病阻止因子の関与 (2012)
  • Author(s): 菅・寺川・杉山・大森・井上・本道
  • Organizer: 日本畜産学会学術集会
  • Place of Presentation: 名古屋大学
  • Year and Date: 2012-03-29
• [Presentation] 非妊娠マウスにおける子宮内膜の形態変化への白血病阻止因子の関与 (2012)
  • Author(s): 菅苗緒美, ら
  • Organizer: 日本畜産学会
  • Place of Presentation: 名古屋大学
  • Year and Date: 2012-03-29
• [Presentation] The regulation of embryo spacing by ihh, BMPs and Wnts in murine uterus (2012)
  • Author(s): Ryosuke Kobayashi (Eiichi Hondo)
  • Organizer: The 4th Congress of Asian Association of Veterinary Anatomist
  • Place of Presentation: Phuket, Thailand
• [Presentation] The effects of IL-11 and CNTF on embryo implantation (2012)
  • Author(s): Chisako Tajima (Eiichi Hondo)
  • Organizer: The 4th Congress of Asian Association of Veterinary Anatomist
  • Place of Presentation: Phuket, Thailand
• [Presentation] マウス子宮上皮細胞の増殖に対する白血病阻止因子の作用 (2011)
  • Author(s): 寺川純平, ら
  • Organizer: 日本獣医学会
  • Place of Presentation: 大阪府立大学
  • Year and Date: 2011-09-20
• [Presentation] マウス子宮上皮細胞の増殖に対する白血病阻止因子の作用 (2011)
  • Author(s): 寺川・杉山・大森・井上・本道
  • Organizer: 日本獣医学会学術集会
  • Place of Presentation: 大阪府立大学
  • Year and Date: 2011-09-09
• [Book] (2014)
  • Author(s): 本道栄一(山本雅子, 谷口和美監訳)
  • Total Pages: 504
  • Publisher: 緑書房
• [Book] 動物発生学 (2014)
  • Author(s): 本道栄一（山本雅子、谷口和美監訳）
  • Total Pages: 504
  • Publisher: 緑書房