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Studies on the immunoinhibitory mechanisms of Marek's disease virus and its application for adjuvant

Research Project

Project/Area Number 23380176
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied veterinary science
Research InstitutionHokkaido University

Principal Investigator

OHASHI KAZUHIKO  北海道大学, (連合)獣医学研究科, 教授 (90250498)

Co-Investigator(Kenkyū-buntansha) KONNAI Satoru  北海道大学, 大学院・獣医学研究科, 准教授 (40396304)
村田 史郎  北海道大学, (連合)獣医学研究科, 助教 (10579163)
Co-Investigator(Renkei-kenkyūsha) MURATA Shiro  北海道大学, 大学院・獣医学研究科, 助教 (10579163)
Project Period (FY) 2011-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥18,460,000 (Direct Cost: ¥14,200,000、Indirect Cost: ¥4,260,000)
Fiscal Year 2013: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2012: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2011: ¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000)
Keywordsマレック病 / マレック病ウイルス / 免疫抑制 / PD-1 / 獣医学 / 免疫抑制因子
Research Abstract

The programmed death 1 (PD-1) receptor and its ligands, programmed death ligand 1 (PD-L1) and 2 (PD-L2) are known to have an immunoinhibitory function which contribute to the pathogenesis of chronic infections and neoplastic diseases via the PD-1/PD-L pathway. In this study, it has been shown that chicken PD-1 and PD-L1/PD-L2 also have an immunoinhibitory function, such as inhibition of cytokine production. The expressions of PD-1 and PD-L2 were significantly higher in the spleens of infected chickens in the latent phase and in tumor lesions caused by Marek's disease virus (MDV). These results suggest that chicken PD-1/PD-L2 pathway is involved in the establishment of latency and tumor formation by MDV.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Annual Research Report
  • 2011 Annual Research Report
  • Research Products

    (23 results)

All 2014 2013 2012 2011

All Journal Article (13 results) (of which Peer Reviewed: 12 results) Presentation (10 results)

  • [Journal Article] Expression analysis of programmed death ligand 2 in tumors caused by the avian oncovirus Marek's disease virus2014

    • Author(s)
      Matsuyama-Kato, A., Murata, S., Isezaki, M., Takasaki, S., Kano, R., Konnai, S., & Ohashi, K
    • Journal Title

      Arch. Virol

      Volume: (印刷中) Issue: 8 Pages: 2123-6

    • DOI

      10.1007/s00705-014-2021-7

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Influence of PD-L1 cross-linking on cell death in PD-L1-expressing cell lines and bovine lymphocytes2014

    • Author(s)
      Ryoyo Ikebuchi, Satoru Konnai, Tomohiro Okagawa, Kazumasa Yokoyma, Chie Nakajima, Yasuhiko Suzuki, Shiro Murata, Kazuhiko Ohashi.
    • Journal Title

      Immunology

      Volume: (印刷中) Issue: 4 Pages: 551-61

    • DOI

      10.1111/imm.12243

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Characterization of Meq proteins from field isolates of Marek’s disease virus in Japan2013

    • Author(s)
      Murata, S., Hashiguchi, T., Hayashi, Y., Yamamoto, Y., Matsuyama-Kato, A., Takasaki, S., Isezaki, M., Onuma, M., Konnai, S., Ohashi, K.
    • Journal Title

      Infect Genet Evol.

      Volume: 16C Pages: 137-143

    • DOI

      10.1016/j.meegid.2012.12.032

    • NAID

      120005317003

    • Related Report
      2013 Annual Research Report 2013 Final Research Report 2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Enhanced expression of LAG-3 on lymphocyte subpopulations from persistently lymphocytotic cattle infected with bovine leukemia virus2013

    • Author(s)
      Konnai, S., Suzuki, S., Shirai, T., Ikebuchi, R., Okagawa, T., Sunden., Y, Mingala C.N., Onuma, M., Murata, S., Ohashi K.
    • Journal Title

      Comp Immunol Microbiol Infect Dis.

      Volume: 36 Issue: 1 Pages: 63-9

    • DOI

      10.1016/j.cimid.2012.09.005

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Blockade of bovine PD-1 increases T cell function and inhibits bovine leukemia virus expression in B cells in vitro2013

    • Author(s)
      Ryoyo Ikebuchi, Satoru Konnai, Tomohiro Okagawa, Kazumasa Yokoyma, Chie Nakajima, Yasuhiko Suzuki, Shiro Murata, Kazuhiko Ohashi.
    • Journal Title

      Veterinary Research

      Volume: 44 Issue: 1 Pages: 59-59

    • DOI

      10.1186/1297-9716-44-59

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Expression analysis of Foxp3 in T cells from bovine leukemia virus infected cattle2013

    • Author(s)
      Saori Suzuki, Satoru Konnai, Tomohiro Okagawa, Ryoyo Ikebuchi, Tatsuya Shirai, Yuji Sunden, Claro N. Mingala, Shiro Murata, Kazuhiko Ohashi
    • Journal Title

      Microbiology and Immunology

      Volume: 57 Pages: 600-604

    • DOI

      10.1111/j.1348-0421.12073

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Molecular characterization of immunoinhibitory factors PD-1/PD-L1 in chickens infected with Marek's disease virus2012

    • Author(s)
      Matsuyama-Kato, A., Murata, S., Isezaki, M., Kano, R., Takasaki, S., Ichii, O., Konnai, S., & Ohashi, K
    • Journal Title

      Virol. J

      Volume: 9 Issue: 1 Pages: 94-94

    • DOI

      10.1186/1743-422x-9-94

    • Related Report
      2013 Final Research Report 2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Increased bovine Tim-3 and its ligand expressions during bovine leukemia virus infection2012

    • Author(s)
      Okagawa, T., Konnai, S., Ikebuchi, R., Suzuki, S., Shirai, T., Sunden, Y., Onuma M., Murata, S., Ohashi, K.
    • Journal Title

      Vet. Res.

      Volume: 43 Issue: 1 Pages: 45-45

    • DOI

      10.1186/1297-9716-43-45

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Surveillance or Marek's disease virus in migratory and sedentary birds in Hokkaido, Japan2011

    • Author(s)
      Murata S., et al.
    • Journal Title

      Veterinary Journal

      Volume: 192(印刷中) Issue: 3 Pages: 538-540

    • DOI

      10.1016/j.tvjl.2011.07.006

    • Related Report
      2013 Final Research Report 2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Analysis of transcriptional activities of the Meq proteins present in highly virulent Marek's disease virus strains, RB1B and Md52011

    • Author(s)
      Murata S., et al.
    • Journal Title

      Virus Genes

      Volume: 43 Issue: 1 Pages: 66-71

    • DOI

      10.1007/s11262-011-0612-x

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Increase of cells expressing PD-L1 in bovine leukemia virus infection and enhancement of anti-viral immune responses in vitro via PD-L1 blockade2011

    • Author(s)
      R.Ikebuchi, S.Konnai, T.Shirai, Y.Sunden, S.Murata, M.Onuma, K.Ohashi
    • Journal Title

      Veterinary Research

      Volume: 42 Issue: 1 Pages: 103-103

    • DOI

      10.1186/1297-9716-42-103

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Molecular cloning of bovine lymphocyte activation gene-3 and its expression characteristics in bovine leukemia virus-infected cattle2011

    • Author(s)
      Shirai T, Konnai S, Ikebuchi R, Okagawa T, Suzuki S, Sunden Y, Onuma M, Murata S, Ohashi K
    • Journal Title

      Vet.Immunol.Immunopathol.

      Volume: 44 Issue: 3-4 Pages: 462-467

    • DOI

      10.1016/j.vetimm.2011.08.018

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] マガンなどの野生水禽の疾病抵抗性とマレック病ウイルスの分布2011

    • Author(s)
      大橋和彦, 松原綾子, 村田史郎, 今内覚
    • Journal Title

      J. V. M

      Volume: 64 Pages: 21-26

    • URL

      http://iss.ndl.go.jp/books/R100000002-I000000010707-00

    • Related Report
      2013 Final Research Report
  • [Presentation] マレック病腫瘍病変形成における免疫抑制因子発現機序の検討2013

    • Author(s)
      松山あゆ美, 村田史郎, 伊勢崎政美, 今内覚, 大橋和彦
    • Organizer
      第156回日本獣医学会学術集会
    • Place of Presentation
      岐阜大学(岐阜)
    • Year and Date
      2013-09-20
    • Related Report
      2013 Final Research Report
  • [Presentation] マレック病腫瘍病変形成における免疫抑制因子発現機序の検討2013

    • Author(s)
      松山 あゆ美、村田 史郎、伊勢崎 政美、今内 覚、大橋 和彦
    • Organizer
      第156回日本獣医学会学術集会
    • Place of Presentation
      岐阜大学(岐阜県)
    • Related Report
      2013 Annual Research Report
  • [Presentation] マレック病感染鶏由来腫瘍病変部における免疫抑制因子PD-1/PD-L 発現の形態学的解析2012

    • Author(s)
      村田史郎, 松山あゆ美, 伊勢崎政美, 高崎紗蘭, 市居修, 今内覚, 大橋和彦
    • Organizer
      第154回日本獣医学会学術集会
    • Place of Presentation
      岩手大学(盛岡)
    • Year and Date
      2012-09-16
    • Related Report
      2013 Final Research Report
  • [Presentation] マレック病ウイルスワクチン接種鶏における免疫抑制因子PD-1 およびPD-L1 の発現解析2012

    • Author(s)
      高崎紗蘭, 村田史郎, 松山あゆ美, 伊勢崎政美, 今内覚, 大橋和彦
    • Organizer
      第154回日本獣医学会学術集会
    • Place of Presentation
      岩手大学(盛岡)
    • Year and Date
      2012-09-16
    • Related Report
      2013 Final Research Report
  • [Presentation] Characterization of Meq proteins from field isolates of Mrek's disease virus in Japan2012

    • Author(s)
      Murata, S., Hashiguchi, T., Hayashi, Y., Yamamoto, Y., Kato, A., Takasaki, S., Isezaki, M., Konnai, S., & Ohashi, K
    • Organizer
      9th International Symposium on Marek's Disease and Avian Herpesviruses
    • Place of Presentation
      Berlin, Germany
    • Year and Date
      2012-06-24
    • Related Report
      2013 Final Research Report
  • [Presentation] Characterization of immunoinhibitory factors PD-1/PD-L1 in chickens infected with Marek's disease virus2012

    • Author(s)
      Matsuyama-Kato, A., Murata, S., Isezaki, M., Kano, R., Takasaki, S., Ichii, O., Konnai, S., & Ohashi, K
    • Organizer
      9th International Symposium on Marek's Disease and Avian Herpesviruses
    • Place of Presentation
      Berlin, Germany
    • Year and Date
      2012-06-24
    • Related Report
      2013 Final Research Report
  • [Presentation] マレック病感染鶏由来腫瘍病変部における免疫抑制因子PD-1/PD-L1発現の形態学的解析2012

    • Author(s)
      村田史郎
    • Organizer
      日本獣医学会学術集会
    • Place of Presentation
      岩手大学(盛岡市)
    • Related Report
      2012 Annual Research Report
  • [Presentation] マレック病ウイルスワクチン接種鶏における免疫抑制因子PD-1およびPD-L1の発現解析2012

    • Author(s)
      髙﨑紗蘭
    • Organizer
      日本獣医学会学術集会
    • Place of Presentation
      岩手大学(盛岡市)
    • Related Report
      2012 Annual Research Report
  • [Presentation] マレック病ウイルス強毒株由来Meq および弱毒株由来L-Meq によるbcl-2 遺伝子発現調節の比較2011

    • Author(s)
      村田史郎, 伊勢崎政美, 松山あゆ美, 高崎紗蘭, 今内覚, 大橋和彦
    • Organizer
      第152回日本獣医学会学術集会
    • Place of Presentation
      大阪府立大学(堺)
    • Year and Date
      2011-09-20
    • Related Report
      2013 Final Research Report
  • [Presentation] Amino acid substitutions or insertion in the Meq proteins could affect their transactivation and transformation abilities2011

    • Author(s)
      Murata, S., Hashiguchi, T., Okada, T., Kano, R., Onuma, M., Konnai, S., & Ohashi, K
    • Organizer
      International Union of Microbiological Societies Congress
    • Place of Presentation
      Sapporo, Japan
    • Year and Date
      2011-09-11
    • Related Report
      2013 Final Research Report

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Published: 2011-04-06   Modified: 2019-07-29  

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