| Budget Amount *help |
¥13,910,000 (Direct Cost: ¥10,700,000、Indirect Cost: ¥3,210,000)
Fiscal Year 2013: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2012: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2011: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
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| Research Abstract |
The aim of this study was to generate a replication-defective (M gene-deficient) rabies virus strain by gene manipulation and to examine the potency of the strain as a rabies vaccine. Our preliminary data strongly suggested that the M gene-deficient virus could not sufficiently propagate in Vero cells stably expressing M protein. We generated a mixture of P- and M-gene deficient viruses by gene manipulation and examined the potency as a rabies vaccine. Theoretically, P- and M-gene deficient viruses will not produce progeny viruses unless the two different viruses infect an identical cell and complement the gene defect of each other. In fact, we found that the mixture of the P- and M-gene deficient viruses has high levels of safety and immunogenicity, indicating that the mixture is a promising candidate as the next-generation rabies vaccine.
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