| Budget Amount *help |
¥18,200,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥4,200,000)
Fiscal Year 2013: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2012: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2011: ¥9,750,000 (Direct Cost: ¥7,500,000、Indirect Cost: ¥2,250,000)
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| Research Abstract |
The results indicated that the stimulation with house dust mite antigen (HDM) induced the production of GM-CSF in canine keratinocytes, which was suppressed by a cysteine protease inhibitor. Thus, it was demonstrated that cysteine protease activity was essential for GM-CSF production in canine kerationocytes activated by HDM.
Papain that was a naturally derived cysteine protease induced nuclear translocation of NFAT. GM-CSF production was partially inhibited by blockade of NFAT with ciclosporin. These results suggest that GM-CSF production mediated by the cysteine protease is regulated not only by NFAT but also by unknown signaling pathways in canine keratinocytes.
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