The mechanism of activation of Langerhans cells mediated by keratinocytes

• Project/Area Number: 23380184
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Clinical veterinary science
• Research Institution: Gifu University
• Principal Investigator: MAEDA SADATOSHI 岐阜大学, 応用生物科学部, 教授 (50377694)
• Co-Investigator(Kenkyū-buntansha): KAMISHINA Hiroaki 岐阜大学, 応用生物科学部, 准教授 (50506847) ; NISHIFUJI Koji 東京農工大学, 農学研究科, 准教授 (20365422) ; MIZUNO Takuya 山口大学, 農学部, 教授 (90398826)
• Project Period (FY): 2011-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥18,200,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥4,200,000); Fiscal Year 2013: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2012: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000); Fiscal Year 2011: ¥9,750,000 (Direct Cost: ¥7,500,000、Indirect Cost: ¥2,250,000)
• Keywords: アレルギー・ぜんそく / 獣医学 / トランスレーショナルリサーチ / アトピー性皮膚炎 / ケラチノサイト

Research Abstract

The results indicated that the stimulation with house dust mite antigen (HDM) induced the production of GM-CSF in canine keratinocytes, which was suppressed by a cysteine protease inhibitor. Thus, it was demonstrated that cysteine protease activity was essential for GM-CSF production in canine kerationocytes activated by HDM.
Papain that was a naturally derived cysteine protease induced nuclear translocation of NFAT. GM-CSF production was partially inhibited by blockade of NFAT with ciclosporin. These results suggest that GM-CSF production mediated by the cysteine protease is regulated not only by NFAT but also by unknown signaling pathways in canine keratinocytes.

Research Products

• [Journal Article] Involvement of nuclear factor of activated T cells in granulocyte-macrophage colony-stimulating factor production in canine keratinocytes stimulated with a cysteine protease (2013)
  • Author(s): Kimura, T., Sekido, M., Iio, A., Chimura, N., Shibata, S., Kamishina H., Maeda, S
  • Journal Title: Vet Dermatol Volume: 24(3) Issue: 3 Pages: 310-314
  • DOI: 10.1111/vde.12017
  • Peer Reviewed
• [Journal Article] Protease-activated receptor-2 induces proinflammatory cytokine and chemokine gene expression in canine keratinocytes (2013)
  • Author(s): Shingo Maeda
  • Journal Title: Veterinary Immunology and Immunopathology Volume: (印刷中) Issue: 1-2 Pages: 17-25
  • DOI: 10.1016/j.vetimm.2013.01.018
  • Peer Reviewed
• [Journal Article] Production of GM-CSF Mediated by Cysteine Protease of Der f in Canine Keratinocytes (2012)
  • Author(s): Kimura, T., Sekido, M., Chimura, N., Shibata, S., Kondo, N., Kamishina, H., Maeda, S
  • Journal Title: Journal of Veterinary Medical Science Volume: 74 Issue: 8 Pages: 1033-1036
  • DOI: 10.1292/jvms.11-0522
  • NAID: 130001879794
  • ISSN: 0916-7250, 1347-7439
  • Peer Reviewed
• [Journal Article] Production of GM-CSF Mediated by Cysteine Protease of Der f 1 in Canine Keratinocytes (2012)
  • Author(s): Tsuyoshi KIMURA
  • Journal Title: Journal of Veterinary Medical Science Volume: (In press)
  • Peer Reviewed
• [Presentation] 犬アトピー性皮膚炎のサイトカイン・ケモカイン (2012)
  • Author(s): 前田貞俊
  • Organizer: 第15回日本獣医皮膚科学会学術大会
  • Place of Presentation: 大宮ソニックシティ(埼玉県)
  • Year and Date: 2012-03-18