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The mechanism of activation of Langerhans cells mediated by keratinocytes

Research Project

Project/Area Number 23380184
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Clinical veterinary science
Research InstitutionGifu University

Principal Investigator

MAEDA SADATOSHI  岐阜大学, 応用生物科学部, 教授 (50377694)

Co-Investigator(Kenkyū-buntansha) KAMISHINA Hiroaki  岐阜大学, 応用生物科学部, 准教授 (50506847)
NISHIFUJI Koji  東京農工大学, 農学研究科, 准教授 (20365422)
MIZUNO Takuya  山口大学, 農学部, 教授 (90398826)
Project Period (FY) 2011-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥18,200,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥4,200,000)
Fiscal Year 2013: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2012: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2011: ¥9,750,000 (Direct Cost: ¥7,500,000、Indirect Cost: ¥2,250,000)
Keywordsアレルギー・ぜんそく / 獣医学 / トランスレーショナルリサーチ / アトピー性皮膚炎 / ケラチノサイト
Research Abstract

The results indicated that the stimulation with house dust mite antigen (HDM) induced the production of GM-CSF in canine keratinocytes, which was suppressed by a cysteine protease inhibitor. Thus, it was demonstrated that cysteine protease activity was essential for GM-CSF production in canine kerationocytes activated by HDM.
Papain that was a naturally derived cysteine protease induced nuclear translocation of NFAT. GM-CSF production was partially inhibited by blockade of NFAT with ciclosporin. These results suggest that GM-CSF production mediated by the cysteine protease is regulated not only by NFAT but also by unknown signaling pathways in canine keratinocytes.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Annual Research Report
  • 2011 Annual Research Report
  • Research Products

    (5 results)

All 2013 2012

All Journal Article (4 results) (of which Peer Reviewed: 4 results) Presentation (1 results)

  • [Journal Article] Involvement of nuclear factor of activated T cells in granulocyte-macrophage colony-stimulating factor production in canine keratinocytes stimulated with a cysteine protease2013

    • Author(s)
      Kimura, T., Sekido, M., Iio, A., Chimura, N., Shibata, S., Kamishina H., Maeda, S
    • Journal Title

      Vet Dermatol

      Volume: 24(3) Issue: 3 Pages: 310-314

    • DOI

      10.1111/vde.12017

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Protease-activated receptor-2 induces proinflammatory cytokine and chemokine gene expression in canine keratinocytes2013

    • Author(s)
      Shingo Maeda
    • Journal Title

      Veterinary Immunology and Immunopathology

      Volume: (印刷中) Issue: 1-2 Pages: 17-25

    • DOI

      10.1016/j.vetimm.2013.01.018

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Production of GM-CSF Mediated by Cysteine Protease of Der f in Canine Keratinocytes2012

    • Author(s)
      Kimura, T., Sekido, M., Chimura, N., Shibata, S., Kondo, N., Kamishina, H., Maeda, S
    • Journal Title

      Journal of Veterinary Medical Science

      Volume: 74 Issue: 8 Pages: 1033-1036

    • DOI

      10.1292/jvms.11-0522

    • NAID

      130001879794

    • ISSN
      0916-7250, 1347-7439
    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Production of GM-CSF Mediated by Cysteine Protease of Der f 1 in Canine Keratinocytes2012

    • Author(s)
      Tsuyoshi KIMURA
    • Journal Title

      Journal of Veterinary Medical Science

      Volume: (In press)

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Presentation] 犬アトピー性皮膚炎のサイトカイン・ケモカイン2012

    • Author(s)
      前田貞俊
    • Organizer
      第15回日本獣医皮膚科学会学術大会
    • Place of Presentation
      大宮ソニックシティ(埼玉県)
    • Year and Date
      2012-03-18
    • Related Report
      2011 Annual Research Report

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Published: 2011-04-06   Modified: 2019-07-29  

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